Chapter 20

Question 1

What are restriction enzymes?

Answer 1

Restriction enzymes cut DNA molecules at specific DNA sequences called restriction sites

Question 2

What do restriction enzymes produce?

Answer 2

A restriction enzyme makes many cuts that yield restriction fragments, if in a staggered way it produces sticky ends

Question 3

What do restriction enzymes produce?

Answer 3

A restriction enzyme makes many cuts that yield restriction fragments, if in a staggered way it produces sticky ends

Question 4

what do sticky ends do?

Answer 4

sticky ends can bond with complementary sticky ends of other fragments

Question 5

what is DNA ligase?

Answer 5

Dna ligase is an enzyme that seals the bonds between restriction fragments

Question 6

What is a totipotent cell?

Answer 6

A totipotent cell can generate a complete new organism; generally in plants

Question 7

what is a pluripotent cell?

Answer 7

embryonic stem cells are pluripotent meaning they are capable of differentiating into many different cell types

Question 8

what is an induced pluripotent stem cell?

Answer 8

ips cells can perform most functions of embryonic stem cells

Question 9

what are multipotent stem cells?

Answer 9

adult stem cells are multipotent, can generate multiple cell types but not all and are used to replace nonreproducing cells; ex: bone marrow can become any blood cell type

Question 10

what are multipotent stem cells?

Answer 10

adult stem cells are multipotent, can generate multiple cell types but not all and are used to replace nonreproducing cells; ex: bone marrow can become any blood cell type

Question 11

did dolly have aging problems?

Answer 11

they showed no signs of premature aging, joint problems from being kept inside and fed too many treats