Chapter 2- Microscopy

Question 1

Use visible light to illuminate specimens

Answer 1

Light microscope

Question 2

Total magnification=

Answer 2

Objective x ocular

Question 3

The ability to distinguish two close objects as separate

Answer 3

Resolution

Question 4

Resolution is determined by blank and blank

Answer 4

Wavelength of light and lenses used

Question 5

A blank wavelength will result in better resolution

Answer 5

Shorter

Question 6

Type of microscope we use in lab

Answer 6

Bright-field microcope

Question 7

Distance between the front surface of the lens and the specimen

Answer 7

Working distance

Question 8

Photos taken with Bright-field microscopes

Answer 8

Photomicrographs

Question 9

Produce a bright image of object against a dark background

Answer 9

Dark-field microscope

Question 10

Used to observe living cells

Answer 10

Phase-contrast microscope

Question 11

Creates image by detecting differences within a species

Answer 11

Differential Interference Contrast Microscope

Question 12

Exposes specimens to ultraviolet, violet, or blue light

Answer 12

Fluorescence Microscope

Question 13

Blank are a type of unstained preparation

Answer 13

Wet mounts

Question 14

Blank and blank are types of stained preparations

Answer 14

Vital stains and fixed and stained slides

Question 15

Dye is added to wet mount to stain living organisms

Answer 15

Vital stains

Question 16

Kills microorganisms and adheres them to slides

Answer 16

Fixation

Question 17

Two types of fixation

Answer 17

Heat and chemical

Question 18

Any distortion in microorganism due to technique

Answer 18

Artifact

Question 19

Coloring ion

Answer 19

Chromophore

Question 20

Chromophore cation is used for

Answer 20

Basic dyes

Question 21

Chromophore anion is used for

Answer 21

Acidic dyes

Question 22

Go over Micro Lab flashcards

Answer 22

Okay

Question 23

Used to stain mycobacterium

Answer 23

Acid-fast staining

Question 24

Differential stain used to stain the endospore one color and vegetative cell another color

Answer 24

Endospore staining

Question 25

Electron Microscopy light=blank, lenses=blank, image=blank, stains=blank

Answer 25

electron beam, magnets, computer, metals

Question 26

Blank microscopy allows for the greatest detail to be viewed because blank

Answer 26

Electron, shortest wavelength of light

Question 27

Heavy metals do not penetrate specimen but stain the background

Answer 27

Negative stain

Question 28

Coating specimen with thin layer of heavy metal on one side

Answer 28

Shadowing

Question 29

Freeze specimen then fracture along lines of greatest weakness, reduces blank

Answer 29

Freeze-etching, artifacts

Question 30

Used to view atoms on the surface of a solid object

Answer 30

Scanning tunneling micrscope

Question 31

Sharp probe moves over specimen at a constant distance

Answer 31

Atomic force microscope

Question 32

Adding microorganisms to culture media

Answer 32

Inoculation

Question 33

Anything done to minimize contamination

Answer 33

Asceptic tecniques