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Microbiology > Chapter 2- Microscopy > Flashcards

Chapter 2- Microscopy Flashcards

1
Q

Use visible light to illuminate specimens

A

Light microscope

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2
Q

Total magnification=

A

Objective x ocular

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3
Q

The ability to distinguish two close objects as separate

A

Resolution

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4
Q

Resolution is determined by blank and blank

A

Wavelength of light and lenses used

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5
Q

A blank wavelength will result in better resolution

A

Shorter

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6
Q

Type of microscope we use in lab

A

Bright-field microcope

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7
Q

Distance between the front surface of the lens and the specimen

A

Working distance

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8
Q

Photos taken with Bright-field microscopes

A

Photomicrographs

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9
Q

Produce a bright image of object against a dark background

A

Dark-field microscope

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10
Q

Used to observe living cells

A

Phase-contrast microscope

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11
Q

Creates image by detecting differences within a species

A

Differential Interference Contrast Microscope

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12
Q

Exposes specimens to ultraviolet, violet, or blue light

A

Fluorescence Microscope

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13
Q

Blank are a type of unstained preparation

A

Wet mounts

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14
Q

Blank and blank are types of stained preparations

A

Vital stains and fixed and stained slides

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15
Q

Dye is added to wet mount to stain living organisms

A

Vital stains

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16
Q

Kills microorganisms and adheres them to slides

A

Fixation

17
Q

Two types of fixation

A

Heat and chemical

18
Q

Any distortion in microorganism due to technique

A

Artifact

19
Q

Coloring ion

A

Chromophore

20
Q

Chromophore cation is used for

A

Basic dyes

21
Q

Chromophore anion is used for

A

Acidic dyes

22
Q

Go over Micro Lab flashcards

A

Okay

23
Q

Used to stain mycobacterium

A

Acid-fast staining

24
Q

Differential stain used to stain the endospore one color and vegetative cell another color

A

Endospore staining

25
Q

Electron Microscopy light=blank, lenses=blank, image=blank, stains=blank

A

electron beam, magnets, computer, metals

26
Q

Blank microscopy allows for the greatest detail to be viewed because blank

A

Electron, shortest wavelength of light

27
Q

Heavy metals do not penetrate specimen but stain the background

A

Negative stain

28
Q

Coating specimen with thin layer of heavy metal on one side

A

Shadowing

29
Q

Freeze specimen then fracture along lines of greatest weakness, reduces blank

A

Freeze-etching, artifacts

30
Q

Used to view atoms on the surface of a solid object

A

Scanning tunneling micrscope

31
Q

Sharp probe moves over specimen at a constant distance

A

Atomic force microscope

32
Q

Adding microorganisms to culture media

A

Inoculation

33
Q

Anything done to minimize contamination

A

Asceptic tecniques

Microbiology (4 decks)

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