Chapter 2- DNA Structure Flashcards

1
Q

________ stroes genetic info and hoe is it passed to daughter cells

A

DNA stores genetic information and is passed to daughter cells through dividing via replication

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2
Q

What is central dogma

A

The passing of info from dna to rna by transcription and then to protein by translation is callled cemtral dogma

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3
Q

Whats the dna model we use now

A

Watson and crick

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4
Q

What does the primary structure of dna have and how does it form sec pr ter

A

It has a sugar phosphate bacbones attached to nitrogenous bases attached to them and aggregation happens to form sec and then tertiary.

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5
Q

What has phosphate and what has base and whats the carbon thats diff and how

A

Phosphate is attachex to 5th carbon and 1st carbon has base and 2nd carbon has o in rna and no o in dna.

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6
Q

Whats a nucleotide

A

A sugar phosphate backbone with a base attaches

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7
Q

What are the two types of bases

A

Pyramidines are cytosines, thymine and uracil that have one cyclic ring and purine is adenine and gusnine containing two cyclic rings

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8
Q

Whats deamination and what does it cause

A

It causes mutation of A to G by removal of amine group and addition of O. Also of pyramidines by removal of amine group from c to and addition of o to form U and further addition if methyl group to form thymine

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9
Q

What are nucleosides and how can they be converted into nculeotides

A

They dont have phosphste bhut have everything else and by adding phosphate they can be converted to nucleotides.

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10
Q

What are mono di and tri phosphates and by what

A

Addition kf single double or triple phosphates by high energy bonds

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11
Q

What are the differences between dna and rna

A

Dna is double stranded and rna is single
Dna has deoxyribose rna has ribose
Dna has no u and t instead however rna has u instead of t

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12
Q

Phosphodiesterbond is in which direction and dna synthesis in which

A

3’-5’ ; 5’-3’

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13
Q

A and t has how many bonds and g and c how many and who requires more energy and why

A

At has two bonds and gc have three and gc take more to breakdown because more bonds

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14
Q

Whats chargaffs rule

A

A = t and g = c in percentage

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15
Q

Dna binds in ________ manner

A

Antipsrellel

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16
Q

What forms of dna

A

Dna has A form that can be dehydrated into b form and b form is 99.9%. Of dna in humans and z form is the one thats having regulatory proterties.

17
Q

What breaks between dna and how

A

Hydrogen bonds betwenen dna strands and by denaturing at high temp or alter8ng the ph.

18
Q

What is melting temp and how can dna be repaured

A

It is at which half of dna is denatured and by cooling

19
Q

Whats the diff between promaryotic dna and eukaryotic

A

Prokaryotic dna is circular small and ds dns that has no membrane bounded organelles. Dna is bound by non histone proteins to form nucloid. They also have plasmids that may replicate and store info just like dna.
Eukaryotes are dsdna which is linear which is bounded by membrane bound organelles and also dna is wrapped in non histone and histone proteins (h2A; H2B; H3 and H4) to form nucleosome which is an example of chromatin.