Chapter 12 test-bank Flashcards
What RNA sequence would be transcribed from this DNA sequence? PR = promoter region; TM = terminator
3’—-PR—-TTCGAGTCGTAGTCAG—TM
5’————AAGCTCAGCATCAGTC——-
Select one:
a. 5’-AAGCUCAGCAUCAGUC
b. 5’- AAGCTCAGCATCAGTC
c. 3’-AAGCTCAGCATCAGTC
d. 3’-AAGCUCAGCAUCAGUC
a. 5’-AAGCUCAGCAUCAGUC
The unit of transcription at the molecular level is the gene.
Select one:
True
False
True
The following are events in prokaryotic transcription. Choose the response that lists these events in the proper order.
i. RNA polymerase and sigma factor scan down the template strand approximately 10 bases.
ii. RNA polymerase choses the appropriate NTPs for the newly synthesizing RNA strand
iii. The promoter region unwinds, breaking the hydrogen bonds between the base pairs.
iv. RNA polymerase releases the template at the termination regionvi. RNA polymerase and the sigma factor bind to the promoter region
v. DNA template and coding strands reform their complementary base pairing
vi. RNA polymerase and the sigma factor bind to the promoter region
Select one:
a. i, iii, v, iv, vi, ii
b. vi, iii, i, ii, iv, v
c. if none of these lists the correct order of events, then choose this response.
d. vi, i, iv, iii, ii, v
b. vi, iii, i, ii, iv, v
Which region of DNA contains the coding information for a protein in a eukaryote? Select one: A. Introns B. Enhancers C. Exons D. Promoters
C. Exons
Transcription in prokaryotes has which of the following features…
Select one or more:
a. Transcription involves copying the entire circular chromosome
b. Transcription occurs in the cytoplasm.
c. Transcription requires a protein factor called the sigma factor to help RNA polymerase recognize the termination region of the gene.
d. Transcription requires a promoter region at the 5’ end of the template DNA strand.
b. Transcription occurs in the cytoplasm.
RNA is complementary to
Select one: A. nontemplate strand of DNA B. The coding strand of DNA C. Regulatory sequences D. The template strand of DNA
D. The template strand of DNA
Which of the following terms is defined as the removal of introns from the pre-mRNA of eukaryotes?
Select one:
a. capping
b. splicing
c. renovating
d. terminating
e. transitioning
b. splicing
The consensus sequences are 5’TTGACA3’ and 5’TATAAT3’ at -35 and -10 respectively. Which of the following would be most easily recognized by σ factor?
Select one: A. 5'TTGAAA3' B. 5'TATATA3' C. 5'TAAATT3' D. 5'TTCAGA3'
A. 5’TTGAAA3’
During transcription,
Select one:
a. the template strand is read in the 3’ to 5’ direction
b. the coding strand is read in the 3’ to 5’ direction
c. the template strand is read in the 5’ to 3’ direction.
d. the coding strand is read in the 5’ to 3’ direction
a. the template strand is read in the 3’ to 5’ direction Correct
Which RNA encodes the sequence of amino acids for a functional protein?
Select one: A. scRNA B. snRNA C. tRNA D. rRNA E. mRNA
E. mRNA
where does the process of transcription initiate?
Select one: A. Regulation sequences B. Terminator C. Transcription factors D. Promoter
D. Promoter
What is the correct order of elements that comprise a functional protein encoding gene in a prokaryote?
Select one:
A. Regulatory region, promoter, terminator, transcribed region
B. Regulatory region, promoter, transcribed region, terminator
C. Promoter, regulatory region, transcribed region, terminator
D. Promoter, transcribed region, regulatory region, terminator
B. Regulatory region, promoter, transcribed region, terminator
The DNA sequence is:
3’ TTCGTTCAAGTC (polynucleotide strand A)
5’ AAGCAAGTTCAG (polynucleotide strand B)
The following RNA sequence was generated from the above DNA:
5’ CUGAACUUGCUU
Which statement is TRUE from the following?
Select one:
a. The DNA polynucleotide strand A is the template strand for this RNA.
b. The DNA polynucleotide strand B is the coding strand for this RNA.
c. The DNA polynucleotide strand A is the template strand for this RNA.
d. If responses “a” and “b” are both TRUE, then choose this response.
e. If none of the responses in this question are TRUE, then choose this response.
a. The DNA polynucleotide strand A is the template strand for this RNA.
Which of the following is a post-transcriptional modification that stabilizes and increases the life span of a eukaryotic mRNA?
Select one: A. Alternative splicing B. 5' capping C. Trimming D. Polyadenylation E. RNA editing
D. Polyadenylation
Which of the following is not a post-transcriptional modification of RNAs in eukaryotes?
Select one:
a. adding a polyA tail to the mRNA
b. removing exons from the RNA
c. removing introns from the mRNA
d. adding a 7methyl-guanine cap to the mRNA
b. removing exons from the RNA
