Chapter 12: Genomes

Question 0

massively parallel sequencing

Answer 0

• used in next generation dna seq

- millions of fragments are sequenced at the same time

Question 1

next-generation dna sequencing

Answer 1

uses miniaturization techniques to sequence dna more efficiently
1. broken into fragments
2. denatured
3. attached to short adapter sequences and then solid support
4. copied around 1000 times by pcr
to sequence:
1. denatured and primer/polymerase/4 nucleotides are added
2. complementary strand is set up
3. florescence is detected
4. florescent tag is removed

Question 2

bioinformatics

Answer 2

field that analyzes dna seq using complex math and computer programs

Question 3

functional genomics

Answer 3

field where biologists use seq info to identify the functions of various parts of genomes

Question 4

open reading frames

Answer 4

the coding regions of genes

can be reorganized

Question 5

comparative genomics

Answer 5

comparison of newly sequenced genes