Chapter 12: Food proteins and enzymes Flashcards
What is a protein good for?
Amino acids, source of calories (energy)
Calories:
amount of heat required to raise 1 g of water 1 degree
1 Calorie=1 kcal=1000 calories
Where is energy stored?
Polysaccharides (glycogen) or fat (triglycerides
Proteins
amino acids
20 amino acids
differ on R structure
Fibrous Protein
insoluble in water
ex: muscle, connective tissue and hair
Globular proteins
soluble in water
ex: eggs, dairy, enzymes, myoglobin
Membrane proteins
not water soluble
transporters, receptors
Primary 1ᵒ structure
the amino acid sequence
Secondary 2ᵒ
local structure
- coils: alpha-helix, “random” coil
- extended: beta-strands (sheets)
- turns
Tertiary 3ᵒ
overall 3-D structure
2 and 3ᵒ fold to produce 3D structure
Quaternary 4ᵒ
multiple protein chains
-proteins can associate with other proteins
Hydrolysis of protiens
- we reverse synthesis by chemically insert a water molecule, break apart the aa’s
we are able to absorb and digest the individual amino acids
Connective tissue
- triple helices of proline-rich peptides
- covalently cross-linked
- assembled into fibers
How is gelatin formed?
collagen structure is “unwound” by heating in hot water
-the backbone forms a sol or gel of gelatin
Red meat
red part is muscle protein + myoglobin
In red meat fat between muscle is called?
seam fat
fat is held by connective tissue
Within the muscle it is called
marbling (often prized)
What is the contractile protein?
actomyosin: composed of several proteins, notably actin and myosin
Myosin
is assembles in muscle fibers
Seed Proteins
bran
germ
aleuron
starchy endosperm
Starchy endosperm
85% of kernel
10-15% is protein
remains in white flour
Prolamins
wheat proteins: storage proteins
gluten
-gliadin (mixture of proteins)
Glutelins
wheat proteins
gluten
storage proteins
-glutenin (also a mixture)
Albumins and globulins
wheat protein
`15% of seed protein
-cellular proteins
-enzymes
Gluten proteins and flour types
high molecular weight glutenins form (S-S) crosslinks during kneading- “visco-elastic” dough
Hard wheat
strong flour
bread flour (10-13% protein)
high gluten protein concentration
Soft wheat
cake flour (8-10% protein)
lower gluten concentration
used for quick breads
minimally kneaded
All-purpose flour
a blend of soft and hard wheat (9-12%)
Enzymes
proteins
-ase
catalyze nearly all the reactions of life
What do enzymes catalyze?
digestion
energy metabolism
biosynthesis
everything
essential amino acids
body can not metabolize or make on its own in sufficient quantities
-must be in diets
9 of them
Limiting amino acids
when a protein is low in one essential amino acid necessary for synthesis, that EAA is the limiting factor
Protein quality
- 9 hens egg
- 1 Cows milk
- 5 fish
- 3 beef
- 3 Soybeans
- 5 Dry beans
- 1 Corn