chapter 11

Question 1

The replication of DNA proceeds with one parental strand used as template to build a new double helix
that will include a parental molecule and a newly synthesized molecule

Answer 1

semiconservative

Question 2

what direction is DNA Replication

Answer 2

Bidirectional

Question 3

Once replication has started, the point where the DNA is separated into two strands, each of which is replicated,
is called

Answer 3

replication fork

Question 4

where replication begins

Answer 4

origin of replication

Question 5

are present to
replicate whole eukaryotic chromosomes

Answer 5

multiple origins of replication

Question 6

All DNA polymerases synthesize a new DNA molecule with
direction

Answer 6

5’ to 3’

Question 7

as only one strand is
synthesized continuously while the other is polymerized in pieces.

Answer 7

semidiscontinuous

Question 8

strand that is synthesized continuously

Answer 8

leading strand

Question 9

synthesized in pieces

Answer 9

lagging strand

Question 10

DNA polymerases require a blank which guides the insertion of new nucleotides

Answer 10

template strand

Question 11

required by DNA polymerases and are elongated

Answer 11

primer strand

Question 12

as it is used to
remove mismatched nucleotides

Answer 12

proofreading with the 3’ to 5’ exonuclease

Question 13

Dissociating completely from the DNA
strand after each insertion.

Answer 13

distributive synthesis

Question 14

Sliding forward of one base pair to
reposition the 3’ terminus

Answer 14

processive synthesis

Question 15

is a heterotrimer composed of
subunits α, ε, and θ.
Alpha: polymerase activity
Epsilon: proofreading 3’5’ activity
Theta: unknown role

Answer 15

Polymerase 3 core

Question 16

is substantially larger
and includes three Pol III cores, two ring-shaped
β sliding clamps

Answer 16

Pol III holoenzyme

Question 17

includes a series
of proteins that ultimately differentiate the
activity of the holoenzyme from that of the
single cores

Answer 17

Pol III holoenzyme

Question 18

The β sliding clamp is a

Answer 18

homodimer

Question 19

separate the two DNA strands via NTP hydrolysis (typically ATP hydrolysis). They bind to a single-
stranded gap in DNA and move along it “propelled” by ATP hydrolysis. As they move, they unwind DNA. They can move in either direction, depending on the type

Answer 19

helicases

Question 20

The Pol III holoenzyme, DnaB and primase constitutes the

Answer 20

replisome

Question 21

the action of a blank is required to reduce the superhelical tension

Answer 21

topoisomerase

Question 22

it binds the DNA helicase for activity. RNA synthesis is less accurate than
DNA synthesis and this is how RNA primers are then recognized and digested

Answer 22

DNaG is the primase in E. coli

Question 23

hat protects the single strand from nucleases. In E. coli, SSB is a tetramer but this quaternary structure changes in other organisms

Answer 23

single-stranded DNA–binding protein (SSB)

Question 24

a helicase complex formed by 6
homologous but not identical subunits (named Mcm2 through Mcm7) with AAA+ activity (ATPase). Like DnaB,
this is a ring-shaped hexamer.

Answer 24

MCM complex

Question 25

his is the truly
active helicase, while MCM alone has modest activity.

Answer 25

CMG complex

Question 26

The largest multi-protein assembly has been
called

Answer 26

replisome progression complex
(RPC)

Question 27

is the functional equivalent
of E. coli SSB

Answer 27

replication
protein A (