Chapter 1: Safety measures in the laboratory Flashcards
what is the function of a goggles
to protect the eyes from splashes of hazardous chemicals substances, reactive metals and piece of broken glass
what is the function of a face mask
to prevent the nose and mouth from inhaling chemicals
what is the function of a gloves
protect the hands when handling hazardous and corrosive chemical substances
what is the function of a lab coat
to protect a student’s clothes from any corrosive chemicals
what is the function of closed shoes
to protect feet from chemical spillage or broken glass pieces
The use of laminar flow cabinet
this cabinet provides sterile space to prevent contamination while conducting experiments that related to microbiology
The use of fume chamber
to conduct experiments that involve volatile, flammable, corrosive or poisionous substances/gases
The use of eyewash station
to rinse/wash eyes that are affected by chemical substances immediately
The use of stafety shower
to rinse and clean clothes/body parts that have come in contact with chemical substances immediately
The use of first aid kit
contains equipment and materials such as plasters, bandage, cotton, scissors and others to treat light injuries
Waste substances that can be disposed into the sink
- in liquid form
- of low concentration
- non-hazardous
example: neutral substances, weak acids and weak alkalis
Waste substances that cannot be disposed into the sink
1) Solid waste
2) chemical waste
3) Concentrated acid and alkalis / pH <5 or pH >9
4) radioactive waste
5)heavy metals
6) toxic substances
Managing biological waste substances
What is category A?
sharp equipments
Example: syringes, needles, broken glass pieces
Managing biological waste substances
What is category B?
Non-sharp solid
Example: tissues, gloves, culture mediums
Managing biological waste substances
What is category C?
Carcasses, organs
Example: experimental animals, laboratory animals
Managing biological waste substances
What is category D?
Liquids
Example: blood, serum
how to manage biological waste substances in category A?
- put into a special container
- not autoclave
- containers are stored in a safe place before disposal
how to manage biological waste substances in category B?
- put into biohazard plastic bags
- autoclaved into the biohazard bin before disposing
how to manage biological waste substances in category C?
- wrapped with tissue paper and packed carefully in a biohazard plastic bag
- frozen before disposing
how to manage biological waste substances in category D?
- decontaminated by autoclave
- disposed firectly into the sewage system through the laboratory sink or toilet
Steps to handle accidents in the laboratory
CHEMICAL SPILLAGE
1) notify the teacher immediately
2) make the spillage area a restricted area
3) form a boundary around the spillage using sand
4) scoop the chemical spillage
5) disposed of it safely
Steps to handle accidents in the laboratory
MERCURY SPILLAGE
1) notify the teacher immediately
2) make the spillage area a restricted area
3) Sprinkle sulphur powder to cover the spillage
4) contact the fire department
Type of fire extinguishers
water, foam, carbon dioxide, dry powder
What are the colour of the fire extinguishers
1) Water
2) foam
3) carbon dioxide
4) dry powder
1) red
2) cream
3) black
4) blue
What does water extinguish
fire that involves solid materials such as paper, wood and clothes
What does foam extinguish
fire that involid sold and liquid substances or flammable gases such as oil, paint and natural gases
What does carbon dioxide extinguish
fire that arises from electric currect, gas or steam
What does dry powder extinguish
all types of fires including that involve metal and non-metal substances
Type of fire and its burning substance
1) class A
2) class B
3) class C
4) class D
5) class E
6) class F
1) flammable solids such as paper, wood and cloth(except metals)
2) flammable liquid such as petrol, kerosene, gasoline and paint
3) Flammable gases such as oxygen, LPG and LNG
4) flammable metals such as lithium, potassium, sodium and magnesium
5) electrical appliances such as sockets, cable, iron and computer
6) fire in the kitchen such as those involving cooking oil fats
