Chapter 1: Safety measures in the laboratory

Question 1

what is the function of a goggles

Answer 1

to protect the eyes from splashes of hazardous chemicals substances, reactive metals and piece of broken glass

Question 2

what is the function of a face mask

Answer 2

to prevent the nose and mouth from inhaling chemicals

Question 3

what is the function of a gloves

Answer 3

protect the hands when handling hazardous and corrosive chemical substances

Question 4

what is the function of a lab coat

Answer 4

to protect a student’s clothes from any corrosive chemicals

Question 5

what is the function of closed shoes

Answer 5

to protect feet from chemical spillage or broken glass pieces

Question 6

The use of laminar flow cabinet

Answer 6

this cabinet provides sterile space to prevent contamination while conducting experiments that related to microbiology

Question 7

The use of fume chamber

Answer 7

to conduct experiments that involve volatile, flammable, corrosive or poisionous substances/gases

Question 8

The use of eyewash station

Answer 8

to rinse/wash eyes that are affected by chemical substances immediately

Question 9

The use of stafety shower

Answer 9

to rinse and clean clothes/body parts that have come in contact with chemical substances immediately

Question 10

The use of first aid kit

Answer 10

contains equipment and materials such as plasters, bandage, cotton, scissors and others to treat light injuries

Question 11

Waste substances that can be disposed into the sink

Answer 11

• in liquid form
• of low concentration
• non-hazardous
  example: neutral substances, weak acids and weak alkalis

Question 12

Waste substances that cannot be disposed into the sink

Answer 12

1) Solid waste
2) chemical waste
3) Concentrated acid and alkalis / pH <5 or pH >9
4) radioactive waste
5)heavy metals
6) toxic substances

Question 13

Managing biological waste substances
What is category A?

Answer 13

sharp equipments
Example: syringes, needles, broken glass pieces

Question 14

Managing biological waste substances
What is category B?

Answer 14

Non-sharp solid
Example: tissues, gloves, culture mediums

Question 15

Managing biological waste substances
What is category C?

Answer 15

Carcasses, organs
Example: experimental animals, laboratory animals

Question 16

Managing biological waste substances
What is category D?

Answer 16

Liquids
Example: blood, serum

Question 17

how to manage biological waste substances in category A?

Answer 17

• put into a special container
• not autoclave
• containers are stored in a safe place before disposal

Question 18

how to manage biological waste substances in category B?

Answer 18

• put into biohazard plastic bags
• autoclaved into the biohazard bin before disposing

Question 19

how to manage biological waste substances in category C?

Answer 19

• wrapped with tissue paper and packed carefully in a biohazard plastic bag
• frozen before disposing

Question 20

how to manage biological waste substances in category D?

Answer 20

• decontaminated by autoclave
• disposed firectly into the sewage system through the laboratory sink or toilet

Question 21

Steps to handle accidents in the laboratory
CHEMICAL SPILLAGE

Answer 21

1) notify the teacher immediately
2) make the spillage area a restricted area
3) form a boundary around the spillage using sand
4) scoop the chemical spillage
5) disposed of it safely

Question 22

Steps to handle accidents in the laboratory
MERCURY SPILLAGE

Answer 22

1) notify the teacher immediately
2) make the spillage area a restricted area
3) Sprinkle sulphur powder to cover the spillage
4) contact the fire department

Question 23

Type of fire extinguishers

Answer 23

water, foam, carbon dioxide, dry powder

Question 24

What are the colour of the fire extinguishers
1) Water
2) foam
3) carbon dioxide
4) dry powder

Answer 24

1) red
2) cream
3) black
4) blue

Question 25

What does water extinguish

Answer 25

fire that involves solid materials such as paper, wood and clothes

Question 26

What does foam extinguish

Answer 26

fire that involid sold and liquid substances or flammable gases such as oil, paint and natural gases

Question 27

What does carbon dioxide extinguish

Answer 27

fire that arises from electric currect, gas or steam

Question 28

What does dry powder extinguish

Answer 28

all types of fires including that involve metal and non-metal substances

Question 29

Type of fire and its burning substance
1) class A
2) class B
3) class C
4) class D
5) class E
6) class F

Answer 29

1) flammable solids such as paper, wood and cloth(except metals)
2) flammable liquid such as petrol, kerosene, gasoline and paint
3) Flammable gases such as oxygen, LPG and LNG
4) flammable metals such as lithium, potassium, sodium and magnesium
5) electrical appliances such as sockets, cable, iron and computer
6) fire in the kitchen such as those involving cooking oil fats