Chapter 1 - Microscopes Flashcards
What is magnification?
A measure of the ability of a lens to magnify the size of something in an optical image
What is resolution?
Refers to the ability of a microscope to distinguish two adjacent points as separate from each other
What are light microscopes used for?
To examine specimens in labs/ to see large cellular structures
What cannot be seen using a light microscope?
Internal structures of organelles
What can be used to aid in viewing microscopic material?
Coloured dyes stain different tissues
How do Transmission electron microscopes work?
Electrons pass through the material, allowing internal structures to be seen
What must be used to aid in viewing microscopic material when using a transmission electron microscope?
Must use specific stains which will absorb the particles (they make parts look black and different shades of grey)
How do scanning electron microscopes work?
Electrons are bounced off the surface of the material, giving a 3d image
Calculation for magnification using lengths?
Length in picture/real length (MIO mag=image/object)
Calculation for magnification using a microscope?
Magnification of eyepiece x magnification of objective lens
Max magnification of a Light microscope?
x1500
Max resolution of a Light microscope?
200nm
Max magnification of a Transmission electron microscope?
x500,000
Max resolution of a Transmission electron microscope?
0.2nm
Max magnification of a Scanning electron microscope?
x100,000
