Chapter 1 - Intro Flashcards
hereditary genetics
- observing phenotypes in offspring to understand gene inheritance
- tracing pedigrees of gene transmission within a family
population genetics
calculating allele frequencies to determine changes in gene pools
molecular genetics
- generating plasmids from recombinant DNA
- studying DNA-protien interactions to understand gene regulation
Chargaff’s discoveries about base pair of DNA
- DNA molecules contain similar numbers of A and T bases and G and C
- proportions of A:T and G:C are similar within members of the same species
- complementary base pairs
Watson and Crick findings from x-ray image of DNA
DNA is shaped like a helix, includes 10 base pairs per repeat, and has a consistent diameter
central dogma of biology
DNA is transcribed into RNA. RNA is translated into an amino acid sequence
Initiation (first step of transcription)
- RNA poly binds to group of transcription factors
- DNA helix unwinds
- RNA synthesis begins
Elongation (second step of transcription)
- DNA poly transverses the DNA template, adding complementary base pairs
- newly transcribed RNA transcript is proofread
termination (third step of transcription)
- RNA poly detaches from DNA
- RNA poly stops adding base pairs at a stop codon
- RNA transcript is released
transcription
takes place in the nucleus and produces mRNA
translation
takes place in ribosomes, produces a protein, and involves tRNA
characteristics required for heredity
- stable enough to store info for long periods
- able to replicate accurately
- capable of change to allow evolution
semiconservative replication
creates 2 new duplexes, one parental strand and one daughter strand
mRNA
- transcribed from DNA
- codons complement DNA
- simple strand
- broken down after translation
tRNA
- used to translate mRNA
- anticodons complement mRNA
- clover-shaped look structure
- reactivate after translation