Ch.5 Enzymes Flashcards
Define enzyme
Biological catalyst that can increase the rate of reaction of a chemical reaction, without being chemically changed or used up by the reaction.
Enzymes are made out of
Proteins
Amylase
Digests starch into maltose
Maltase
Digests maltose into glucose
Protease
Digests proteins into amino acids
Lipase
Digests fats into fatty acids and glycerol
Enzymes - about its specificity
Complementary shape of a substrate that can fit into the active site of an enzyme
Carbohydrases
Digests carbohydrates
Substrate
Substance which the enzyme acts on
Active site
Depression on the surface
Characteristics of enzymes
- Catalyse reversible reactions
- Speed up rate of chemical reactions
- Remain unchanged at the end of the chemical reaction
- A small amount of enzyme can catalyse a large number of chemical reactions eg washing powder and a large load of clothes
Factors affecting enzymes - pH
- Enzyme that works best in acidic solutions: pepsin in the stomach
- Enzyme that works best in alkaline solutions: intestinal enzymes
- Maximum activity for an enzyme = optimum pH
- Denatured = Substrate no longer able to fit into an active site. This is irreversible
Factors affecting enzymes - pH
- Enzyme that works best in acidic solutions: pepsin in the stomach
- Enzyme that works best in alkaline solutions: intestinal enzymes
- Maximum activity for an enzyme = optimum pH
- Denatured = Substrate no longer able to fit into an active site. This is irreversible
Facts affecting enzymes - Temperature
- For most enzymes, the optimum temperature is about 40-45 degrees Celsius (although everything else says 37 degrees)
- Lower temp - enzyme inactive
- Higher temp - enzyme denatured as there is a rapid decrease in the rate of enzyme reaction. High kinetic energy breaks down enzyme bonds and enzyme loses shape and active site.
- Most enzymes are denatured above 60 degrees Celsius