Ch11

Question 1

genomics
Why is this important

Answer 1

the study of complete genome sequences
helps us understand evolution and identify various factors such as rare mutations diseases, etc.

Question 2

genome library

Answer 2

cloned DNA fragments from an organism’s genetic material (the whole genome of an organism is cloned)

Question 3

Sanger sequencing

Answer 3

(Also known as dideoxy sequencing) method utilized to identify bases

Question 4

When does automated sequencing/cycle-sequencing methods occur?

Answer 4

when larger DNA fragments (applies to Sanger method) go through longer electrophoretic separation.

Question 5

What if the cloned fragment of DNA is longer than what can be experienced?

Answer 5

Primer walking occurs, where new primers are designed after previous sequences, in order to move farther

Question 6

What is the estimated limit of cloned DNA in a vector?

Answer 6

10kb for most cloning plasmids
45kb for cosmids

Question 7

WHAT is high throughput sequencing

Answer 7

new sequencing technology that did not rely on gel electrophoresis like the Sanger (also known as next generation sequencing)

Question 8

Has DNA sequencing improved from when it was introduced

Answer 8

Obviously

Question 9

What is an advantage of high throughput sequencing?

Answer 9

sequences can be collected from various molecules ate the same time