ch 5 Neisseria

Question 1

Neisseria spp gram stain

Answer 1

Gram negative, diplococci (kidney shaped).

Question 2

Growth temp for Pathogens and Commensals

Answer 2

37°C

20-42°C Optimal temp. 37°C

Question 3

CO2 requirement

Answer 3

pathogenes: 5-10% CO2

Commensals : Do not need

Question 4

media for pathogenes

Answer 4

Grow on chocolate chocolate agar
agar

Question 5

media Commensals

Answer 5

Can grow on nutrient agar, blood agar, chocolate agar, Muller-Hinton agar

Question 6

TM medium

Answer 6

Pathogens : can grow

Commensals : Cannot grow except Neisseria lactamica

Question 7

Chocolate Agar + 5-10% CO2 for ______ e.g.

Answer 7

for sterile samples

g. blood culture
and CSF)

Question 8

Thayer Martin Agar (TM) + 5-10% CO2 (for ____
e.g.)

Answer 8

for unsterile samples

e.g. urethral swab and Cervical swab

Question 9

Colony:

Answer 9

small colorless or grey colonies.

Question 10

Source of 5-10% CO2

Answer 10

1. CO2 incubator 2. Jar + CO2 gas pack 3. Candle jar

Question 11

Biochemical Reactions

Answer 11

• Catalase positive
• Oxidase positive

Question 12

is used to identify bacteria containing the respiratory
enzyme

Answer 12

The oxidase test

Question 13

Positive result in Oxidase Test

Answer 13

à purple color in 15 seconds.

Question 14

Sugar fermentation test
• Indicator:

Answer 14

phenol red

Question 15

N. gonorrhoeae ferments

Answer 15

glucose only.

Question 16

N. meningitidis ferments

Answer 16

glucose and maltose.

Question 17

N. lactamica ferments

Answer 17

glucose, maltose and lactose.

Question 18

Neisseria gonorrhoeae cause

Answer 18

Causes gonorrhea

Question 19

It has vaccine because the virulence factor for it is the pili صح او خطا

Answer 19

خطا

Question 20

What are the complications of gonorrhea?

Answer 20

infertility

Question 21

Virulence factors of N. gonorrhoeae:

Answer 21

Polyphosphates

Pili
IgA protease

Question 22

Specimens Neisseria gonorrhoeae

Answer 22

Urethral swab

Cervical swab

Rectal swab

Throat swab

Blood culture

Question 23

Neisseria meningitidis cause

Answer 23

It causes meningococcal meningitis and acute purulent meningitis

Question 24

Sources of infection Neisseria meningitidis

Answer 24

Case

Carrier

Question 25

12 serogroups have been identified

Answer 25

A, B, C, Y, and W135.

Question 26

Capsular antigen Groups

Answer 26

A, B and C are responsible for epidemics of meningitis

Question 27

Polysaccharides of groups

Answer 27

A, C, Y, and W135 are immunogenic –
used as vaccines

Question 28

Group B is

Answer 28

nonimmunogenic

Question 29

Types of vaccines:

Answer 29

Groups A, C, AC, and ACYW135

ACW135Y (expensive, difficult to obtain

Group B has vaccine independent of the capsule
o 4 component MenB

Question 30

Virulence factors of N. meningitidis

Answer 30

Polysaccharide capsule

Pili

LOS

Question 31

Symptoms of Meningitis:

Answer 31

• Severe headache • Stiff neck • Fever

Question 32

Complications of N. meningitidis

Answer 32

Waterhouse-Friderichsen syndrome (WFS)

Question 33

Specimens of N. meningitidis

Answer 33

Nasopharyngeal swab à culture on TM •
CSF à culture on chocolate agar •
Blood culture à culture on chocolate agar