ch 5 Neisseria Flashcards

1
Q

Neisseria spp gram stain

A

Gram negative, diplococci (kidney shaped).

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2
Q

Growth temp for Pathogens and Commensals

A

37°C

20-42°C Optimal temp. 37°C

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3
Q

CO2 requirement

A

pathogenes: 5-10% CO2

Commensals : Do not need

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4
Q

media for pathogenes

A

Grow on chocolate chocolate agar
agar

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5
Q

media Commensals

A

Can grow on nutrient agar, blood agar, chocolate agar, Muller-Hinton agar

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6
Q

TM medium

A

Pathogens : can grow

Commensals : Cannot grow except Neisseria lactamica

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7
Q

Chocolate Agar + 5-10% CO2 for ______ e.g.

A

for sterile samples

g. blood culture
and CSF)

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8
Q

Thayer Martin Agar (TM) + 5-10% CO2 (for ____
e.g.)

A

for unsterile samples

e.g. urethral swab and Cervical swab

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9
Q

Colony:

A

small colorless or grey colonies.

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10
Q

Source of 5-10% CO2

A
  1. CO2 incubator 2. Jar + CO2 gas pack 3. Candle jar
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11
Q

Biochemical Reactions

A

• Catalase positive
• Oxidase positive

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12
Q

is used to identify bacteria containing the respiratory
enzyme

A

The oxidase test

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13
Q

Positive result in Oxidase Test

A

à purple color in 15 seconds.

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14
Q

Sugar fermentation test
• Indicator:

A

phenol red

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15
Q

N. gonorrhoeae ferments

A

glucose only.

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16
Q

N. meningitidis ferments

A

glucose and maltose.

17
Q

N. lactamica ferments

A

glucose, maltose and lactose.

18
Q

Neisseria gonorrhoeae cause

A

Causes gonorrhea

19
Q

It has vaccine because the virulence factor for it is the pili صح او خطا

20
Q

What are the complications of gonorrhea?

A

infertility

21
Q

Virulence factors of N. gonorrhoeae:

A

Polyphosphates

Pili
IgA protease

22
Q

Specimens Neisseria gonorrhoeae

A

Urethral swab

Cervical swab

Rectal swab

Throat swab

Blood culture

23
Q

Neisseria meningitidis cause

A

It causes meningococcal meningitis and acute purulent meningitis

24
Q

Sources of infection Neisseria meningitidis

A

Case

Carrier

25
12 serogroups have been identified
A, B, C, Y, and W135.
26
Capsular antigen Groups
A, B and C are responsible for epidemics of meningitis
27
Polysaccharides of groups
A, C, Y, and W135 are immunogenic – used as vaccines
28
Group B is
nonimmunogenic
29
Types of vaccines:
Groups A, C, AC, and ACYW135 ACW135Y (expensive, difficult to obtain Group B has vaccine independent of the capsule o 4 component MenB
30
Virulence factors of N. meningitidis
Polysaccharide capsule Pili LOS
31
Symptoms of Meningitis:
• Severe headache • Stiff neck • Fever
32
Complications of N. meningitidis
Waterhouse-Friderichsen syndrome (WFS)
33
Specimens of N. meningitidis
Nasopharyngeal swab à culture on TM • CSF à culture on chocolate agar • Blood culture à culture on chocolate agar