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بكتيريا 1 > ch 1 Staphylococci > Flashcards

ch 1 Staphylococci Flashcards

1
Q

Staphylococci is gram ___

A

gram postive coccus

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2
Q

Staphylococci motlition

A

non-motile

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3
Q

s.aureus is

A

nasal carrier

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4
Q

identification straphylococci

A

Gram positive, cocci in clusters shape (grapes).

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5
Q

micrococci

A

gram postive cocci in trated

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6
Q

Staphylococci Catalase

A

+ve

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7
Q

Staphylococci Oxidation Fermentation Test

A

facultative anaerobic

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8
Q

Staphylococci Oxidase

A

negative

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9
Q

Staphylococci Bacitracin

A

Resistant

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10
Q

Staphylococci Lysostaphin

A

sensitive

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11
Q

Micrococci Catalase

A

postive

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12
Q

Micrococci Oxidation Fermentation Test

A

aerobic

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13
Q

Micrococci Bacitracin

A

sensitive

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14
Q

Micrococci Lysostaphin

A

resistant

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15
Q

Mannitol Salt Agar It is

A

a selective medium for S. aureus.

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16
Q

Selective agent:

A

7-9% NaCl

17
Q

Indicator:

A

phenol red.

18
Q

is an enzyme which catalyzes the dissociation of hydrogen peroxide (H2O2) to oxygen and water.

A

Catalase

19
Q

Catalase Test Substrate:

A

H2O2

20
Q

Catalase Test End product (

A

positive result): gas bubbles (O2)

21
Q

is an enzyme that converts fibrinogen to fibrin, which means that it can coagulate plasma. The test is used to distinguish between S. aureus from coagulase-negative Staphylococci.

A

Coagulase

22
Q

Coagulase Test
Substrate:

A

plasma fibrinogen.

23
Q

DNase Test substrate

A

DNA

24
Q

can be used to differentiate between potentially pathogenic S. aureus and other Staphlococcus by looking for the presence of the exo-enzyeme DNase. After growth for 24-48 hrs 1N HCL is added to the agar plate.

A

DNase agar

25
Q

DNase Positive result:

A

zone of clearance around the colony.

26
Q

Dnase Negative result:

A

no zone of clearance around the colony.

27
Q

It is a rapid latex agglutination test for identification of Staphylococcus aureus. Latex particles coated with human fibrinogen for detection of clumping factor and coated with specific IgG for detection of protein A and surface antigens characteristic of S. aureus.

A

Staphaurex

28
Q

is a major cause of nosocomial infection worldwide. It is resistant to I
a number of widely used antibiotics due to acquisition of penicillin binding Msft
protein 2a encoded by mecA gene and production of β-lactamases

A

MRSA

29
Q

Treatment of MRSA

A

Vancomycin

30
Q

Virulence factors of S. aureus:

A

Enterotoxin
Adhesins
Catalase
7- Coagulase

31
Q

Diseases by s.aureus

A

abscess
pneumonia
urinary tract infection,
Food poisoning

32
Q

Treatment if S. aureus sensitive to methicillin (MSSA).

A

Floxacillin

33
Q

Micrococci Oxidase

A

postive

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