ch 1 Staphylococci Flashcards
Staphylococci is gram ___
gram postive coccus
Staphylococci motlition
non-motile
s.aureus is
nasal carrier
identification straphylococci
Gram positive, cocci in clusters shape (grapes).
micrococci
gram postive cocci in trated
Staphylococci Catalase
+ve
Staphylococci Oxidation Fermentation Test
facultative anaerobic
Staphylococci Oxidase
negative
Staphylococci Bacitracin
Resistant
Staphylococci Lysostaphin
sensitive
Micrococci Catalase
postive
Micrococci Oxidation Fermentation Test
aerobic
Micrococci Bacitracin
sensitive
Micrococci Lysostaphin
resistant
Mannitol Salt Agar It is
a selective medium for S. aureus.
Selective agent:
7-9% NaCl
Indicator:
phenol red.
is an enzyme which catalyzes the dissociation of hydrogen peroxide (H2O2) to oxygen and water.
Catalase
Catalase Test Substrate:
H2O2
Catalase Test End product (
positive result): gas bubbles (O2)
is an enzyme that converts fibrinogen to fibrin, which means that it can coagulate plasma. The test is used to distinguish between S. aureus from coagulase-negative Staphylococci.
Coagulase
Coagulase Test
Substrate:
plasma fibrinogen.
DNase Test substrate
DNA
can be used to differentiate between potentially pathogenic S. aureus and other Staphlococcus by looking for the presence of the exo-enzyeme DNase. After growth for 24-48 hrs 1N HCL is added to the agar plate.
DNase agar