Ch. 5 Microbial Metabolism Flashcards
Metabolic Pathway
A sequence of chemical reactions inside a cell
Metabolism
All the chemical reactions inside a cell
Anabolism
Chemical reactions that use NRG to make bigger molecules from smaller ones
Catabolism
Chemical reactions that release NRG by breaking chemical bonds
Endergonic
Requires NRG to occur, products have more NRG than reactants
Exergonic
Releases NRG, products have less NRG than reactants
Enzymes
Catalyze chemical reactions in a living cell
Active Site
Where substrate binds to enzyme-binding is specific
Inhibitor
Substance that binds to an enzyme and lowers activity
Competitive Inhibitors
Competes with substrate for active site
Noncompetitive Inhibitors
Binds to enzyme at a location other than the active site
Allosteric Site
On/Off switch
Activator
Substance that binds to an enzyme and increases activity
Cofactors
Molecules that help enzymes
Oxidation
Atom loses e-
Reduction
Atom gains e-
Biological Oxidation
Dehydrogenation bc H atom is also lost w/ e-
Substrate-level Phosphorylation
Enzyme transfers a phosphate group from an organic molecule to ADP, seen in Glycolysis.
Oxidative Phosphosrylation
Last step of respiration where H+ ions moved across membrane by ETC provide NRG to ATP Synthase so it can add iP to ADP, process requires a final e- acceptor like Oxygen or another molecule.
Embden-Meyerhof
Most common form of glycolysis as route for glucose and other hexoses
Entner-Doudoroff
Used by G- bac soil and a few G+ bac
Makes 1 net ATP
Pentose-Phosphate
Used aerobically or anaerobically and at same time as EMP or EDP
What are the steps of Respiration?
- Glycolysis
- Oxidation of Pyruvate
- Kreb’s Cycle
- ETC
What happens in Oxidation of Pyruvate?
IN: Pyruvate
OUT: Acetyl CoA, CO2, and NADH