Ch. 5 Microbial Metabolism Flashcards

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0
Q

Metabolic Pathway

A

A sequence of chemical reactions inside a cell

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1
Q

Metabolism

A

All the chemical reactions inside a cell

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2
Q

Anabolism

A

Chemical reactions that use NRG to make bigger molecules from smaller ones

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3
Q

Catabolism

A

Chemical reactions that release NRG by breaking chemical bonds

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4
Q

Endergonic

A

Requires NRG to occur, products have more NRG than reactants

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5
Q

Exergonic

A

Releases NRG, products have less NRG than reactants

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6
Q

Enzymes

A

Catalyze chemical reactions in a living cell

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7
Q

Active Site

A

Where substrate binds to enzyme-binding is specific

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8
Q

Inhibitor

A

Substance that binds to an enzyme and lowers activity

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9
Q

Competitive Inhibitors

A

Competes with substrate for active site

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10
Q

Noncompetitive Inhibitors

A

Binds to enzyme at a location other than the active site

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11
Q

Allosteric Site

A

On/Off switch

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12
Q

Activator

A

Substance that binds to an enzyme and increases activity

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13
Q

Cofactors

A

Molecules that help enzymes

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14
Q

Oxidation

A

Atom loses e-

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15
Q

Reduction

A

Atom gains e-

16
Q

Biological Oxidation

A

Dehydrogenation bc H atom is also lost w/ e-

17
Q

Substrate-level Phosphorylation

A

Enzyme transfers a phosphate group from an organic molecule to ADP, seen in Glycolysis.

18
Q

Oxidative Phosphosrylation

A

Last step of respiration where H+ ions moved across membrane by ETC provide NRG to ATP Synthase so it can add iP to ADP, process requires a final e- acceptor like Oxygen or another molecule.

19
Q

Embden-Meyerhof

A

Most common form of glycolysis as route for glucose and other hexoses

20
Q

Entner-Doudoroff

A

Used by G- bac soil and a few G+ bac

Makes 1 net ATP

21
Q

Pentose-Phosphate

A

Used aerobically or anaerobically and at same time as EMP or EDP

22
Q

What are the steps of Respiration?

A
  1. Glycolysis
  2. Oxidation of Pyruvate
  3. Kreb’s Cycle
  4. ETC
23
Q

What happens in Oxidation of Pyruvate?

A

IN: Pyruvate
OUT: Acetyl CoA, CO2, and NADH

24
Q

What happens in the Kreb’s Cycle?

A

IN: Last 2 Carbons of Pyruvate carried by Acetyl CoA
OUT: NADH, FADH2, 2 ATP, CO2

25
Q

The final electron acceptor in Aerobic Cellular Respiration is what?

A

Oxygen

26
Q

The final electron acceptor in Anaerobic Cellular Respiration is what?

A

Inorganic or Organic; not oxygen

27
Q

What are 2 common types of Fermentation?

A
  1. Alcoholic Fermentation

2. Lactic Acid Fermentation

28
Q

What happens in Alcoholic Fermentation?

A

Pyruvate reduced to CO2 and Ethanol

29
Q

What happens in Lactic Acid Fermentation?

A

Pyruvate reduced to lactic acid
Homolactic(just lactic acid)
Heteroactic(lactic+other acids)

30
Q

Extracellular Lipases

A

Breakdown lipids to fatty acids and glycerol

31
Q

Beta Oxidation

A

oxidation of fatty acids into 2 carbon units, enters Kreb’s Cycle as Acetyl CoA

32
Q

Example Tests

A

Often detect by production of certain chemical products that cause a color change bc of a change in pH

33
Q

Amination

A

Add amino group to pyruvate or another organic acid

34
Q

Transamination

A

Remove amino group from old aa