Ch 49 Sample Collection and Handling Flashcards
What are the common locations of parasite infections in animals?
Oral cavity, esophagus, stomach, small and large intestine, and other internal organs.
How are parasites detected in fecal samples?
Through microscopic examination.
What life stages of parasites can be found in feces?
- Eggs
- Oocysts
- Larvae
- Segments (tapeworms)
- Adult organisms.
What methods are used to detect external parasites?
- Skin scrapings
- Cellophane tape preparations
- Vacuuming
- Brushing the coat.
What is the best type of fecal sample for analysis?
Fresh samples.
What should be done if fecal samples cannot be analyzed within a few hours?
Refrigerate or mix with 10% formalin.
Why might older fecal samples be problematic for parasite detection?
The appearance of eggs, oocysts, or other life cycle stages may be altered due to parasite development.
What is the recommended size of a fecal sample for analysis?
About 1 g (thimble-sized).
How should fecal samples be labeled?
Properly labeled.
What is a method for collecting large animal fecal samples?
Collected directly from the animal’s rectum or pooled samples from many animals.
What equipment is needed for skin scraping?
- Electric clipper with a No. 40 blade
- Scalpel or spatula
- Mineral oil in small dropper bottle.
What is the purpose of scraping skin lesions?
To evaluate animals with external parasites.
What should be done during skin scraping to ensure effective collection?
Scrape until capillary blood oozes.
What method is used for diagnosing surface mites?
Cellophane tape preparation.
How is flea debris collected for analysis?
Brush hair with a fine tooth flea comb and place debris on white paper towel.
What is the purpose of spraying flea debris with water?
To check for redness indicating dried blood from flea excrement.
How can parasites present on the skin surface be collected?
Using a vacuum with filter paper over the attachment.
What must be ensured when collecting samples at necropsy?
Samples must be properly contained, labeled, and shipped.
What two methods can be used for recovering samples from the digestive tract?
- Decanting
- Sieving.
What should be used to preserve samples from the digestive tract?
70% alcohol or 10% formalin.
What is a critical consideration when handling bladder worms?
They may be zoonotic.
What equipment is required for blood sample collection?
Sterile equipment and alcohol.
What is one method for collecting blood samples?
By syringe and needle or Vacutainer.
What is necessary for the diagnosis of alimentary parasitism?
Fecal collection.
True or False: Fecal samples from large animals are often collected as pooled samples.
True.
Fill in the blank: Deep skin scraping is required to collect parasites that reside in _______.
[hair follicles].
