Ch. 3 Review Flashcards

1
Q

What is dialysis?

A

Diluting small molecules out of a dialysis bag and leaving protein molecules.

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2
Q

What is gel-filtration?

A
  • it is based on size of molecule
  • basically a diffusion of small and large molecules through carb polymers to separate protein molecules
  • large molecules make it to bottom 1st because they are large and pass by the polymers
  • *small molecules are small enough and have to go through the polymers loops so it takes longer henceforth they are last
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3
Q

What is ion-exchange?

A
  • anion + cation exchanges

- positively charge AA bind to negative charged beads while negative charged AA flow through

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4
Q

What is affinity?

A

Addition of glucose to separate glucose binding AA from glucose residues on beads

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5
Q

What is isoelectric focusing?

A

An electrophoretic technique for the separation of proteins based on their isoelectric point (pI).

The pI is the pH at which a protein has NO NET CHARGE and thus, does not migrate further in an electric field.

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6
Q

What does SDS-PAGE stand for?

A

Sodium dodecyl sulfate - polyacrylamide gel electrophoresis

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7
Q

What does ELISA stand for?

A

Enzyme-linked immunosorbent assay

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8
Q

Difference between ELISA and western blotting?

A

ELISA is more rapid + requires less steps

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9
Q

What do ELISA and western blotting have in common?

A

Both are used to detect antibodies

*HIV testing

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10
Q

Methods of protein sequencing

A

Edman degradation

Mass spec-ESI OR MALDI-TOF

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11
Q

How does cyanogen bromide work?

A

Cleaves the carboxyl side of methionine residues

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12
Q

How does trypsin work?

A

Cleaves carboxyl sides of lysine and arginine residues

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13
Q

How does Chymotrypsin work?

A

Cleaves carboxyl side of tyrosine, tryptophan, phenylalanine, leucine, and methionine

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14
Q

How does carboxypeptidase A work?

A

Cleaves carboxyl side of all amino acids EXCEPT arginine, lysine, or proline.

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15
Q

Function of DTT and BME?

A

Breaks di-sulfide bonds

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16
Q

How can we determine a 3D structure of a protein?

A

X-Ray crystallography and NMR