Ch 29 Transcription and Regulation of Gene Expression Flashcards
1
Q
What dictate the gene products are needed at the moment? (2)
A
metabolic condition and growth status
2
Q
What are the 3 major classes of RNA?
A
tmr
3
Q
What direction does RNA polymerase move?
A
3’ to 5’
4
Q
In what direction does RNA chain grow?
A
5’ to 3’
5
Q
What make the polymerase reaction thermodynamically favorable?
A
hydrolysis of PPi to inorganic phosphate
6
Q
What is holoenzyme?
A
apoenzyme = enzyme’s catalytically inactive protein component
holoenzyme = enzyme’s catalytically active form, which includes apoenzyme and cofactor
7
Q
What does the sigma subunit of RNA polymerase do?
A
recognize promoter (specific DNA sequence)
8
Q
Can transcription occur without sigma subunit?
A
yeah… it just that core polymerase (alpha2-beta-beta’) cannot start the process at specific sites
