Ch. 21 - Nucleic Acids and Protein Synthesis Flashcards
structure of a nucleotide includes a
- nitrogen-containing base.
- sugar.
- phosphate group.
There are two types of nucleic acids:
deoxyribonucleic acid (DNA), the genetic material in the
nucleus of a cell, and ribonucleic acid (RNA), which
interprets the genetic information in DNA for the synthesis
of protein.
both are unbranched polymers of repeating monomer units
known as nucleotides.
DNA and RNA
- a base that contains nitrogen.
- a five-carbon sugar.
- a phosphate group.
nucleotide
- are large molecules
- are found in the nuclei of cells
- store information and direct activities for cellular growth and reproduction.
Nucleic acids
The bases in DNA and RNA are:
• derivatives of the heterocyclic amines ___ or ___.
• ___ with a single ring containing two nitrogen
atoms.
- ___ with two rings, each containing two nitrogen atoms.
- H+ acceptors at the ___ atoms in each base.
• derivatives of the heterocyclic amines pyrimidine or purine.
• pyrimidines with a single ring containing two nitrogen
atoms.
- purines with two rings, each containing two nitrogen atoms.
- H+ acceptors at the nitrogen atoms in each base.
KNOW DIFFERENCE BETWEEN NUCLEOSIDE AND NUCLEOTIDE
(NUCLEOSIDE DOES NOT HAVE PHOSPHATE GROUP)
_____ DOES NOT HAVE PHOSPHATE GROUP
NUCLEOSIDE
Does pyrimidine or Purine have more cyclic rings?
Purine has two rings and Pyrimidine has one
In DNA,
• the ___ bases with double rings are adenine (A) and guanine (G).
• the ___ bases with single rings are cytosine (C) and thymine (T).
In DNA,
• the purine bases with double rings are adenine (A) and guanine (G).
• the pyrimidine bases with single rings are cytosine (C) and thymine (T).
In RNA,
- the ____ bases with double rings are adenine (A) and guanine (G).
- the ___ bases with single rings are cytosine (C) and uracil (U).
In RNA,
- the purine bases with double rings are adenine (A) and guanine (G).
- the pyrimidine bases with single rings are cytosine (C) and uracil (U).
In DNA,
• the purine bases with double rings are….
• the pyrimidine bases with single rings are….
In DNA,
• the purine bases with double rings are adenine (A) and guanine (G).
• the pyrimidine bases with single rings are cytosine (C) and thymine (T).
In RNA,
- the purine bases with double rings are …
- the pyrimidine bases with single rings are ….
In RNA,
- the purine bases with double rings are adenine (A) and guanine (G).
- the pyrimidine bases with single rings are cytosine (C) and uracil (U).
The five-carbon sugar
- in RNA is ___
- in DNA is deoxyribose, with no O atom on ___.
• has carbon atoms numbered with primes to distinguish
them from the atoms in the bases.
The five-carbon sugar
- in RNA is ribose.
- in DNA is deoxyribose, with no O atom on C2′.
• has carbon atoms numbered with primes to distinguish
them from the atoms in the bases.
• is composed of a nitrogen-containing base and a sugar, either ribose or deoxyribose.
A nucleoside
• has a base linked by a β-N-glycosidic bond to C1′ of a sugar (ribose or deoxyribose).
A nucleoside
A nucleotide has a phosphate group attached to the
C5′ — OH group of a nucleoside.
The addition of a phosphate to a nucleoside forms a
nucleotide.
The name of a nucleoside that contains a purine ends with
osine.
The name of a nucleoside that contains a pyrimidine ends
with
idine.
The names of DNA nucleosides add ___-to the beginning of their names.
deoxy
The corresponding nucleotides in RNA and DNA are
named by adding _____ to the end of the nucleoside name.
monophosphate
Look at table 21.2 on page 14 of pdf
Nerd Alert!
Adding a phosphate group to AMP forms the diphosphate, ADP.
• Adding a phosphate group to ADP forms the triphosphate, ATP.
DUH
In the primary structure of nucleic acids, each sugar in a sugar–phosphate backbone is attached to a
base .
In the primary structure of nucleic acids,
• the nucleotides are joined by…
phosphodiester bonds.
In the primary structure of nucleic acids,
• the 3ʹ —OH group of the sugar in one nucleotide bonds
to the
phosphate group on the 5ʹ carbon atom in the
sugar of the next nucleotide.
Each nucleic acid has its own unique sequence of bases, which
• is known as its primary structure.
• carries the genetic information.
• is read from the sugar with the free ___ to ___
• is read from the sugar with the free 5ʹ phosphate to the
sugar with the free 3ʹ —OH group.
In the primary structure of RNA, A, C, G, and U are connected by
3ʹ,5ʹ phosphodiester linkages.
Sc ientists determined that • adenine is paired (1:1) with thymine. • guanine is paired (1:1) with cytosine.
This relationship can be summarized: Number of purine molecules = Number of pyrimidine molecules
YEAH WE KNOW
DNA contains complementary base pairs in which adenine is always linked by
two hydrogen bonds to thymine (AT).
DNA contains complementary base pairs in which guanine is always linked by
three hydrogen bonds to cytosine (GC).
consists of two strands of nucleotides that form a
double helix structure like a spiral staircase.
- has two strands held together by the hydrogen bonds between the bases AT and GC.
- has bases along one strand that complement the bases along the other.
DNA DOUBLE HELIX
• the strands in the original or parent DNA molecule
separate to allow the synthesis of complementary DNA
strands.
• the process begins with the unwinding of the double
helix by breaking the hydrogen bonds between the
complementary bases.
• the resulting single strands act as templates for the
synthesis of new complementary strands of DNA.
DNA replication
Wi thin the nucleus,
• nucleoside triphosphates (dATP, dTTP, dGTP, and
dCTP) on the template strand form hydrogen bonds with
their complementary bases.
• phosphodiester linkages are formed between the
nucleotides as the hydrogen bonds form between the
base pairs:
T forms hydrogen bonds with A. G forms hydrogen bonds with C.
Hydrogen Bonding, Base Pairs
- one strand of the double helix is from the parent DNA.
- one is a newly synthesized DNA strand.
• two new daughter DNA strands that are exact copies of
the parent DNA are formed.
• complementary base pairing ensures the correct placement of bases in the daughter DNA strands.
Daughter DNA Strands
During DNA replication,
- _____ unwinds the parent DNA at several sections.
- _____ catalyzes the replication process at each of the open DNA sections called replication forks.
- the polymerase moves in the _____, catalyzing the formation of new ____ linkages.
• the lagging strand (growing in the ___ direction) is
synthesized in short sections called ____ fragments.
• ____ joins the Okazaki fragments.
During DNA replication,
• helicase unwinds the parent DNA at several sections.
• DNA polymerase catalyzes the replication process at
each of the open DNA sections called replication forks.
• the polymerase moves in the 3′–5′direction, catalyzing
the formation of new phosphodiester linkages.
• the lagging strand (growing in the 5′–3′ direction) is
synthesized in short sections called Okazaki
fragments.
• DNA ligase joins the Okazaki fragments.
READ TABLE 21.4 ON PAGE 38 OF PDF ENSURE YOU’RE GOOD WITH IT
K DAD WE KNOW