CH 10-11 worksheet Flashcards
What is the coding strand?
One half of a DNA molecule (always double-stranded)
Coding strand is used as a template for mRNA transcription.
What is the sequence of the template strand?
3’ TCC CGC AGA TCC CTA CAT GGT TAT GCG CAC TGG TAC CGT TTT AAT GCC CGA TGC TAG GCT CAA AAT GGA GGC TTC AAC GGT GTT GAC CAT GGT ATC TTT CGT TTA GTC CTC 5’
Template strand is complementary and anti-parallel to the coding DNA strand.
What is the sequence of the mRNA that would be transcribed from this DNA?
5’ AU GUA CCA AUA CGC GUG ACC AUG GCA AAA UUA CGG GCU ACG AUC CGA GUU UUA CCU CCG AAG UUG CCA CAA CUG GUA CCA UAG AAA GCA AAU CAG GAG 3’
What protein product would be produced from the mRNA molecule?
Met-Ala-Lys-Leu-Arg-Ala-Thr-Ile-Arg-Val-Leu-Pro-Pro-Lys-Leu-Pro-Gln-Leu-Val-Pro
What is the sequence of each anticodon?
All listed 3’ to 5’:
* UAC
* CGU
* UUU
* AAU
* GCC
* CGA
* UGC
* UAG
* GCU
* CAA
* AAU
* GGA
* GGC
* UUC
* AAC
* GGU
* GUU
* GAC
* CAU
* GGU
If there was a deletion of the A base at position +29, what is the sequence of the coding strand?
5’ AGG GCG TCT AGG GAT GTA CCA ATA CGC GTG ACC ATG GCA AAXT TAC GGG CTA CGA TCC GTG TTT TAC CTC CGA TGT TGC CAC AAC TGG TAC CAT AGA AAG CAA ATC AGG AG 3’
What type of mutation is represented by the deletion of the A base at position +29?
Point mutation, deletion, and read-through
What would the sequence of the mRNA be if the deletion was present?
5’ AU GUA CCA AUA CGC GUG ACC AUG GCA AAU UAC GGG CUA CGA UCC GUG UUU UAC CUC CGA UGU UGC CAC AAC UGG UAC CAU AGA AAG CAA AUC AGG AG 3’
What would the sequence of the protein be with this deletion?
Met- Ala- Asn-Tyr- Gly- Leu- Arg- Ser- Val- Phe- Tyr- Leu- Arg- Cys- Cys- His- Asn- Trp- Tyr- His- Arg- Lys- Gln- Ile- Arg
If an animal or plant was heterozygous for this deletion, what protein(s) would be produced?
½ Normal protein:
* Met-Ala-Lys-Leu-Arg-Ala-Thr-Ile-Arg-Val-Leu-Pro-Pro-Lys-Leu-Pro-Gln-Leu-Val-Pro
½ Abnormal (mutated protein):
* Met- Ala- Asn-Tyr- Gly- Leu- Arg- Ser- Val- Phe- Tyr- Leu- Arg- Cys- Cys- His- Asn- Trp- Tyr- His- Arg- Lys- Gln- Ile- Arg
What is the sequence of the non-template strand (if written backwards)?
5’ GAG GAC TAA ACG AAA GAT ACC ATG GTC AAC ACC GTT GAA GCC TCC ATT TTG AGC CTA GCA TCG GGC ATT AAA ACG GTA CCA GTG CGC ATA ACC ATG TAG GGA TCT 3’
What is the sequence of the template strand for the non-template strand provided?
3’ CTC CTG ATT TGC TTT CTA TGG TAC CAG TTG TGG CAA CTT CGG AGG TAA AAC TCG GAT CGT AGC CCG TAA TTT TGC CAT GGT CAC GCG TAT TGG TAC ATC CCT AGA 5’
What is the sequence of the mRNA that would be transcribed from this non-template DNA?
5’ AA ACG AAA GAU ACC AUG GUC AAC ACC GUU GAA GCC UCC AUU UUG AGC CUA GCA UCG GGC AUU AAA ACG GUA CCA GUG CGC AUA ACC AUG UAG GGA UCU 3’
What protein product would be produced from the mRNA molecule transcribed from the non-template strand?
Met- Val- Asn- Thr- Val- Glu- Ala- Ser- Ile- Leu- Ser- Leu- Ala- Ser- Gly- Ile- Lys- Thr- Val- Pro- Val- Arg- Ile- Thr- Met
If there was a deletion of the G base at position +27, what is the sequence of the non-template strand?
5’ GAG GAC TAA ACG AAA GAT ACC ATG GTC AAC ACC TTG AAG CCT CCA TTT TGA GCC TAG CAT CGG GCA TTA AAA CGG TAC CAG TGC GCA TAA CCA TGT AGG GAT CT 3’
What type of mutation is represented by the deletion of the G base at position +27?
Deletion, point mutation, nonsense, frameshift
What would the sequence of the mRNA be if the deletion was present?
5’ AA ACG AAA GAU ACC AUG GUC AAC ACC UUG AAG CCU CCA UUU UGA GCC UAG CAU CGG GCA UUA AAA CGG UAC CAG UGC GCA UAA CCA UGU AGG GAU CU 3’
What would the sequence of the protein be with this deletion?
Met- Val- Asn- Thr- Leu- Lys- Pro- Pro- Phe
If an animal or plant was heterozygous for this deletion, what protein(s) would be produced?
Met- Val- Asn- Thr- Leu- Lys- Pro- Pro- Phe and
Met- Val- Asn- Thr- Val- Glu- Ala- Ser- Ile- Leu- Ser- Leu- Ala- Ser- Gly- Ile- Lys- Thr- Val- Pro- Val- Arg- Ile- Thr- Met
