Central Dogma (Becker) Flashcards
Describe initiation, elongation, and termination
**Note: Please know the composition of RNA polymerase (core, holo, and sigma association)
and that there are mechanisms for initiation (sigma factor), elongation and termination.
RNA polymerase composition
Core RNA polymerase – composted of 4 subunits: 2 x α, β, and β’
Sigma factor associates, the holoenzyme binds to DNA
Compare Eukaryotic and Prokaryotic Translation.
Describe wobble base pairing?
Translation in Prokaryotes?
Eukaryotic translation compared to Prokaryotic.
What is Elongation
What is Termination?
Does the beginning of RNA synthesis require a primer?
No, not like DNA replication
Difference between post-Transcription modifications for Eukaryotes and Prokaryotes? Monosistronic vs. Polycistronic? Name 3 RNA processing in Eukaryotes that increases RNA stability and aids in translation.
What is a target for antibiotics? IMPORTANT
Translation.
Describe tRNA codons and the wobble effect
Sense vs antisense strands
The sense strand has the information that would be readable on the RNA, and that’s called the coding side. The antisense is the non-coding strand, but ironically, when you’re making RNA, the proteins that are involved in making RNA read the antisense strand in order to create a sense strand for the mRNA.
Prokaryotic translation: Start codon What is the tRNA (UAC) attached to? What is the functional ribosome composed of?
Describe prokaryotic translation initiation, what are the 3 ribosome subsections?
Eukaryotic translation similarities
Describe prokaryotic elongation and termination
DNA replication is thought to proceed how out of latency in VZV? What happens if demand is high?
