Central Dogma Flashcards
DNA
Deoxyribonucleic acid
Double helix
Polymer of nucleotides (nucleotides are covalently linked together in a single strand)
A,T,G,C
Gene
Unit of genetic information that provides instructions for a specific property of an organism
Provides the instructions to make a protein
Central Dogma
DNA -> RNA -> Protein
Transcription Translation
Place where we can treat disease (antibiotics)
Transcription factors
Proteins at bind to the regulatory sequence. Of DNA to control gene expression
Gene expression can be positively or negatively regulated
Negative: when the binding of a repressor protein inhibits inscription
Positive: when the binding of an activator protein stimulates transcription
Regulatory region
Turn on/off gene
Cell specific regulation and combination control
Differential expression of genes located in different cells varies because of differing amounts and identities of activators present
Transcription
Process of synthesizing mRNA from a gene
3 steps:
- Initiation
- Elongation
- Termination
3 steps of transcription
- Initiation
- Elongation
- Termination
mRNA
Carries information from the gene to the site of protein synthesis
RNA v DNA
RNA DNA
single stranded Double helix
U T
Ribose Deoxyribose
Goal of translation
Read the sequence of one biological polymer to synthesize another->generate a polypeptide chain
How?
Tools
Code
Ribosome
Tool of translation The molecule that binds to mRNA, decodes it, and covalently links amino acids together Complex of protein and RNA 2 subunits 30s and 50s Acts as an enzyme
tRNA
Tool of translation
Acts as an adapter allowing the ribosome to decode mRNA into a polypeptide chain
Codon
Set of 3 nucleotides read together that code for a specific amino acid
Translation
The process of reading the mRNA and synthesizing the corresponding polypeptide chain
3 key steps:
- Initiation
- Elongation
- Termination
Peptide bond
A specific covalent linkage between two amino acids
Polypeptide
Many amino acids linked together
A polymer of amino acids
Protein
Polymer of amino acids