Cellular Respiration Flashcards

1
Q

What do all living cells need to work

A

Energy from an outside source

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2
Q

Where does energy from organic molecules come from

A

The sun

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3
Q

How is energy from photosynthesis recycled

A

Photosynthesis = oxygen and organic materials -> used for cellular respiration -> breaks down fuel = generates ATP, carbon dioxide and water = used for photosynthesis

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4
Q

Why are catabolic pathways useful in cellular respiration

A

Transfer of electrons from fuel plays a major role.
Linked to work by drive of ATP.
Regenerates ATP from ADP + Pi

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5
Q

What is aerobic respiration

A

Form of cellular respiration.
Oxygen is consumed as reactant.
Carried out by most eukaryotes and prokaryotes.
Organic compounds + oxygen —> carbon dioxide + water + energy

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6
Q

Main sources of fuel

A

Carbohydrates, fats and protein

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7
Q

How can a substance be reduced but not actually lose an electron

A

Some reactions change the degree of electron sharing in covalent bonds eg methane combustion

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8
Q

Why is hydrogen a good fuel for oxidation

A

Bonds are an excellent source of hilltop electrons.

Energy released when electrons fall down the electron gradient during oxygen transfer.

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9
Q

Why is the activation energy barrier important in carbohydrates and fats

A

Without, glucose would combine almost instantly with oxygen

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10
Q

How is glucose stored as energy as NADH

A

Glucose + NAD+ —dehydrogenase—> C=O + NADH + H+
Electrons are oxidised from glucose and passed through the electron carrier.
Then dehydrogenased.
Coenzymes deliver electrons and protons to NAD+.
Releasing NADH + H+

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11
Q

Why is NADH a neutral molecule

A

2 negative electrons are added and 1 proton is added

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12
Q

State the stages of aerobic cellular respiration

A

Glycolysis
Pyruvate oxidation
Citric acid cycle
Oxidative phosphorylation

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13
Q

Where does glycolysis occur

A

In the cytosol

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14
Q

Where does the Krebs cycle and oxidative phosphorylation occur

A

In the mitochondrial membrane

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15
Q

Where are the proteins built into in eukaryotes and prokaryotes

A

Eukaryotes: the inner membrane of the mitochondria
Prokaryotes: the plasma membrane

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16
Q

Roughly, how does the electron transport chain work

A

Electrons removed from glucose move to the top high-energy tier via NADH.
At the bottom low-energy tier, oxygen captures electrons.
Electrons move from one carrier to the other in redox reactions.

17
Q

Why isn’t oxygen and hydrogen explosive in cellular respiration

A

Hydrogen is derived from organic materials instead of H2.

Steps in ETC break the fall of electrons to oxygen, releasing energy in the steps.

18
Q

What is the energy investment and payoff phase

A

Investment - spends ATP
Payoff - pays off ATP from substrate level phosphorylation and NAD+ is reduced to NADH by electrons from oxidation of glucose

19
Q

Steps of glycolysis

A

Phosphorylation of glucose to glucose phosphate.
Splitting the glucose = triose-phosphate.
Oxidation of triode-phosphate.
Production of ATP.

20
Q

Why do you phosphorylise glucose

A

To make glucose more reactive.
Provide energy to activate glucose.
Lower the activation energy.

21
Q

How is triose-phosphate made

A

Phosphorylised glucose is split into 2 3-carbon molecules.

22
Q

How is triose-phosphate oxidised

A

Hydrogen is removed from each TP molecule and transferred to a hydrogen-carrier molecule
NAD+ —> NADH

23
Q

How is pyruvate made

A

From triose-phosphate

24
Q

How does pyruvate enter the mitochondria from the cytosol and why

A

Via active transport when oxygen is present because pyruvate is a charged molecule, so needs a transport protein.

25
What main 3 reactions occur in the Krebs cycle
Pyruvate carboxyl fully oxidises, gives off CO2 when entering mitochondria. 2-carbon fragment is oxidised to store energy - NAD+ —> NADH. Coenzyme A is attached via a sulphur atom to 2-carbon intermediate = acetyl-CoA, which enters the citric acid cycle.
26
Roughly describe the 8 steps of the citric acid cycle
1. Acetyl CoA adds acetyl group to oxaloacetate = citrate 2. Citrate is converted to isocitrate 3. Isocitrate is oxidised, NAD+ —> NADH, loss of CO2 4. Loss of CO2, NAD+ —> NADH 5. CoA is displaced by a phosphate group, GDP —> GTP 6. 2 hydrogens are transferred, FAD —> FADH2 7. Addition of water rearranges bonds in substrate 8. Substrate is oxidised, NAD+ —> NADH and regenerates oxaloacetate
27
How many molecules of ATP do you have after the citric acid cycle is complete
4 ATP molecules
28
what advantages does the folding of the cristae have on the electron transport chain
Provides space for thousands of copies. | Well suited for sequential redox reactions.
29
What is tightly bound to proteins in the ETC that are essential for catalytic functions
Prosthetic groups eg coenzymes and cofactors
30
What occurs down the ETC
Electrons drop in free energy. Carriers alternate between oxidised and reduced states. Last carrier passes electrons to oxygen.