Cells & Viruses Flashcards
What are the two types of cells
Prokaryotic and eukaryotic
Give 3 examples of eukaryotic cells
Animal cells, plant cells, fungal cells
What are the three types of microscopes
Light microscope
Electron microscope
Transmission electron microscope
Define the term resolution
The ability to discriminate fine detail, two neighbouring points are seen as separate
Why does the electron microscope have better resolution?
Electrons have a shorter wavelength than light
What is the main advantage of using a light microscope?
Living processes such as mitosis can be observed
The interior of an electron microscope is a vacuum therefore the specimens have to be dead
Give 3 comparisons of light microscopes and transmission electron microscopes
Light:light reflected by glass lens
TEM:beams refracted by electromagnetic lenses
Light:Low magnification 1500X maximum
TEM: High magnification 1 000 000X maximum
Any others from:
TEM have higher resolution
Image formed on the retina from Light microscopes but imagine is formed on fluorescent screen in TEM
Light microscopes can view living specimens, TEM can’t
What is the difference in the scanning electron microscope (SEM) and transmission electron microscope (TEM)?
TEM specimens are coated in a film of gold so electrons reflect off the surface to create a 3D effect image
Describe the process of homogenisation
Place chopped up specimen into a cold isotonic buffer solution
Blend the tissue to break open cells releasing the organelle
Filter the mixture to remove debris
Describe the process of centrifugation
Mixture is spun so the denser parts are thrown to the bottom, forming a sediment
The supernatant is poured into a tube leaving the sediment ( contains the nucleus )
This is repeated at higher speeds multiple times to retrieve different organelles
Describe a safety precaution of using a centrifuge
Having an equal mass/ counter balance
Why is it a cold isotonic buffer solution?
Cold- to reduce enzyme activity which could break down organelles
Isotonic- to prevent the organelles bursting from osmosis
Buffer- to maintain a constant pH so enzymes/proteins aren’t denatured
Compare the cell walls in eukaryotic cells
Animal- none
Plant- cellulose
Fungal- chitin
Describe the types of granules found in eukaryotic cells
Animal- Glycogen
Plant- Starch
Fungal- Glycogen
Which eukaryotic cells have lysosomes
Animal and fungal
Which eukaryotic cells have vacuoles
Plant
Fungal
Describe the structure of the nucleus
Largest organelle enclosed within a double membrane , contains chromatin and nucleoli
Outline the function of the nucleus
Nuclear pores allow large molecules in and out of the nucleus.
Nucleolus synthesis ribosomal RNA and manufactures ribosomes
Describe the structure of ribosomes
Small bodies of protein and RNA
Either attached to RER or free in cytoplasm
Outline the function of ribosomes
Site of polypeptide synthesis
Free ribosomes will produce proteins that will function within the cytoplasm
Describe the structure of RER
It is a membrane system with flattened sacs, it continues with the outer nuclear membrane, covered with ribosomes
Describe the role of RER
The polypeptides made on the ribosomes accumulate in the rough ER to be passed on,in vesicles, to the Golgi apparatus
Describe the structure of SER
membrane system of interconnecting tubules
Describe the role of SER
synthesis of lipids and their distribution throughout the cell