Cells Flashcards

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1
Q

Similarities

Both prokaryotic and eukaryotic cells contain ___

The cell-surface membrane in eukaryotic and prokaryotic cells is made of …

This bilayer is responsible for …

A

Similarities

Both prokaryotic and eukaryotic cells contain organelles.

The cell-surface membrane in eukaryotic and prokaryotic cells is made of a phospholipid bilayer.

This bilayer is responsible for controlling the passage of substances across exchange surfaces.

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2
Q

Differences

Prokaryotic cells make up ..(e.g. ___).

In contrast, eukaryotic cells make up … e.g. …).

Eukaryotic cells are …

Cells arise from other cells by … in prokaryotic cells and by … in eukaryotic cells.

A

Differences

Prokaryotic cells make up single-celled prokaryotic organisms (e.g. bacteria).

In contrast, eukaryotic cells make up complex eukaryotic organisms (e.g. animals, plants, fungi and algae).

Eukaryotic cells are larger and more complex than prokaryotic cells.

Cells arise from other cells by binary fission in prokaryotic cells and by mitosis or meiosis in eukaryotic cells.

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3
Q

Animal cell organelles:

A

Animal cell organelles:

Cytoplasmic membrane
Endoplasmic reticulum
Ribosomes
Nucleus
Nucleolus 
Nuclear membrane
Golgi
Cytoplasm
Mitochondrion
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4
Q

Plant cell

Plant cells contain all the organelles found in animal cells.

Plant cells also possess:

___ (…).

___ (…)

___ (…)

A

Plant cell

Plant cells contain all the organelles found in animal cells.

Plant cells also possess:

Vacuole (a repository of cell sap).

Chloroplasts (the site of photosynthesis).

Cell wall (made of cellulose and contains plasmodesmata, through which cells exchange substances with each other).

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5
Q

Fungal cells are similar to plant cells except … and …

A

Fungal cells are similar to plant cells except there are no chloroplasts in fungal cells and the cell wall is made from chitin instead of cellulose.

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6
Q

… This is called specialisation. Specialised cells are then …

A

In complex multicellular organisms, cells gain specific features. This is called specialisation. Specialised cells are then organised into groups to perform a function

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7
Q

Cell specialisation

A cell specialises because …

Examples include:

Muscle cells …

Red blood cells …They also …

Palisade cells …

A

Cell specialisation

A cell specialises because the shape and contents of a cell help it to carry out its function.

Examples include:

Muscle cells are very active so contain lots of mitochondria in order to produce ATP.

Red blood cells have a biconcave shape and no nucleus to maximise space to carry oxygen. They also have lots of haemoglobin.

Palisade cells have a long, upright shape and contain chlorophyll to absorb light for photosynthesis.

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8
Q

Cell organisation

Specialised cells are organised into ____, ___ into ___ and ___ into …

Tissues (e.g. …).

Organs (e.g. …).

Organ systems (e.g. …).

A

Cell organisation

Specialised cells are organised into tissues, tissues into organs and organs into organ systems.

Tissues (e.g. muscle tissue, xylem tissue).

Organs (e.g. the animal heart or plant leaf).

Organ systems (e.g. the female reproductive system, which includes the uterus, ovaries, mammary glands and breasts).

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9
Q

Structure of the plasma membrane

The plasma membrane is made from a …

A phospholipid is a …

Plasma membranes have …

These proteins can … This means that …

A

Structure of the plasma membrane

The plasma membrane is made from a phospholipid bilayer.

A phospholipid is a lipid molecule with glycerol, two fatty acid chains, and a phosphate-containing group.

Plasma membranes have proteins and cholesterol embedded in them.

These proteins can act as receptors. This means that the cell can respond to the external environment of the cell.

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10
Q

Function of the plasma membrane

The plasma membrane controls …

Waste products (e.g. ___ and ___) leave …

A

Function of the plasma membrane

The plasma membrane controls the passage of organic molecules, ions, water, and oxygen into and out of the cell.

Waste products (e.g. carbon dioxide and ammonia) leave the cell by passing through the plasma membrane.

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11
Q

Structure of the cytoplasm

Cytoplasm is made up of ____ suspended in …

__% of the cytoplasm is …

The cytoplasm also …

A

Structure of the cytoplasm

Cytoplasm is made up of organelles suspended in the gel-like cytosol.

70% of the cytoplasm is made up of water.

The cytoplasm also contains proteins, sugars, ions and fatty acids.

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12
Q

Functions of the cytoplasm

… take place in the cytoplasm.

Different organelles …

It is important to remember that …

Organelles are NOT cells even though …

A

Functions of the cytoplasm

Many metabolic reactions take place in the cytoplasm.

Different organelles perform specific functions within the cytoplasm.

It is important to remember that organelles are parts of a cell.

Organelles are NOT cells even though they often surrounded by membranes.

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13
Q

Structure of the nuclear envelope

The nuclear envelope is …

Both the inner and outer membranes of the nuclear envelope are …

A

Structure of the nuclear envelope

The nuclear envelope is a double-membrane structure that has a number of pores.

Both the inner and outer membranes of the nuclear envelope are phospholipid bilayers.

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14
Q

Function of the nuclear envelope

The pores in the nuclear envelope control …

A

Function of the nuclear envelope

The pores in the nuclear envelope control the passage of ions, molecules and RNA between the nucleoplasm and cytoplasm.

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15
Q

Structure of the nucleus

The nucleoplasm is …. where we find …

In ____, the nucleus contains …

There is an area within the nucleus called the ____ (plural = nucleoli).

A

Structure of the nucleus

The nucleoplasm is the semi-solid fluid inside the nucleus, where we find the chromatin and the nucleolus.

In eukaryotes, the nucleus contains linear chromosomes that are made up of DNA.

There is an area within the nucleus called the nucleolus (plural = nucleoli).

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16
Q

Function of the nucleus

The nucleus controls …

DNA in the nucleus contains …

___ ___ is joined together with associated proteins … to

A

Function of the nucleus

The nucleus controls the actions of the cell.

DNA in the nucleus contains instructions for the synthesis of proteins.

Ribosomal RNA is joined together with associated proteins in the nucleolus to assemble the ribosomal subunits.

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17
Q

Mitochondria produce … via …

Chloroplasts are … that …

A

Mitochondria produce adenosine triphosphate (ATP) via aerobic respiration.

Chloroplasts are plant cell organelles that carry out photosynthesis.

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18
Q

Structure of mitochondria

Mitochondria are … that have …

Each membrane is …

The inner layer of the membrane …

The area surrounded by the folds is …

The mitochondrial matrix contains …

A

Structure of mitochondria

Mitochondria are oval-shaped, double-membrane organelles that have their own ribosomes and DNA.

Each membrane is a phospholipid bilayer embedded with proteins.

The inner layer of the membrane has folds called cristae.

The area surrounded by the folds is called the mitochondrial matrix.

The mitochondrial matrix contains enzymes used for respiration.

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19
Q

Function of mitochondria

Mitochondria make ATP via aerobic respiration.

Muscle cells have a very high concentration of ___ because … and …

A

Function of mitochondria

Mitochondria make ATP via aerobic respiration.

Muscle cells have a very high concentration of mitochondria because they are highly active and need a lot of energy to keep the body moving.

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20
Q

Structure of chloroplasts

Chloroplasts have … and have …

The space enclosed by the inner membrane contains a set of … and … called ___

Each stack of thylakoids is called a ___(plural = grana).

Grana are linked by ____

Lamellae are …

The fluid enclosed by the inner membrane that surrounds the grana is called the ___

A

Structure of chloroplasts

Chloroplasts have their own DNA, ribosomes and have inner and outer membranes.

The space enclosed by the inner membrane contains a set of interconnected and stacked fluid-filled membrane sacs called thylakoids.

Each stack of thylakoids is called a granum (plural = grana).

Grana are linked by lamellae.

Lamellae are flat, thin parts of thylakoid membrane.

The fluid enclosed by the inner membrane that surrounds the grana is called the stroma.

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21
Q

Function of chloroplasts

Photosynthesis is the series of …

This process happens in ___ and …

Chloroplasts are found in … but not in …

A

Function of chloroplasts

Photosynthesis is the series of reactions that use carbon dioxide, water and light energy to make glucose and oxygen.

This process happens in chloroplasts and allows plants to make their own food (e.g. sugars).

Chloroplasts are found in plant and algal cells but not in animal or fungal cells.

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22
Q

Structure of Golgi apparatus

The Golgi apparatus is …

A

Structure of Golgi apparatus

The Golgi apparatus is a series of flattened membranous sacs.

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23
Q

Function of Golgi apparatus

Transport vesicles form at … and fuse with

The transport vesicles empty ___ and ___ into …

As the ___ and ___ travel through .., they are … so that …

A

Function of Golgi apparatus

Transport vesicles form at the endoplasmic reticulum and fuse with the Golgi apparatus.

The transport vesicles empty proteins and lipids into the lumen of the Golgi apparatus.

As the proteins and lipids travel through the Golgi, they are sorted, packaged and tagged so that they can be sent to the right place.

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24
Q

Structure of Golgi vesicles

Golgi vesicles … located in …

They are ___, ___ and are seen in a high ___ near the ..

___ are a type of Golgi vesicle.

A

Structure of Golgi vesicles

Golgi vesicles are membrane-bound, fluid-filled vesicles located in the cytoplasm.

They are small, round and are seen in a high density near the edges of the sacs.

Lysosomes are a type of Golgi vesicle.

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25
Q

Function of Golgi vesicles

Golgi vesicles …

___ are a special type of Golgi vesicle.

They contain enzymes called ___

Lysozymes aid the …

A

Function of Golgi vesicles

Golgi vesicles store and transport modified proteins and lipids from the Golgi apparatus to target cells.

Lysosomes are a special type of Golgi vesicle.

They contain enzymes called lysozymes.

Lysozymes aid the breakdown of proteins, polysaccharides, lipids, nucleic acids and old organelles.

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26
Q

Ribosomes and the Endoplasmic Reticulum

Proteins are made in ___

Proteins may then be … by the …

The smooth endoplasmic reticulum makes and processes ___

A

Ribosomes and the Endoplasmic Reticulum

Proteins are made in ribosomes.

Proteins may then be folded and processed by the rough endoplasmic reticulum (RER).

The smooth endoplasmic reticulum makes and processes lipids.

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27
Q

Structure of ribosomes

Ribosomes can be … in the cytoplasm or attached to …

Ribosomes are very ___ organelles made of …

This means that ribosomes are not covered by …

A

Structure of ribosomes

Ribosomes can be free floating in the cytoplasm or attached to the cytoplasmic side of the endoplasmic reticulum (ER).

Ribosomes are very small organelles made of protein subunits.

This means that ribosomes are not covered by a membrane.

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28
Q

Function of ribosomes

Ribosomes are in charge of …

… is an essential function of all cells.

This is why ribosomes are found in practically every cell.

A

Function of ribosomes

Ribosomes are in charge of protein synthesis.

Protein synthesis is an essential function of all cells.

This is why ribosomes are found in practically every cell.

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29
Q

Structure of the ER

The ER is a series of …

The membrane of the ER is a … embedded with ___

The smooth ER (SER) membrane has no ___ but the rough ER (RER) membrane has many ___ on it’s ___

A

Structure of the ER

The ER is a series of interconnected membranous sacs and tubules.

The membrane of the ER is a phospholipid bilayer embedded with proteins.

The smooth ER (SER) membrane has no ribosomes but the rough ER (RER) membrane has many ribosomes on its surface.

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30
Q

Function of the RER and SER

The RER is responsible for …

The SER is responsible for …

A

Function of the RER and SER

The RER is responsible for processing and folding proteins.

The SER is responsible for making and processing lipids.

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31
Q

Cell Wall and Cell Vacuole

The cell wall is a structure external to …

Cell vacuoles are found in …

A

Cell Wall and Cell Vacuole

The cell wall is a structure external to the plasma membrane.

Cell vacuoles are found in plant cell cytoplasm.

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32
Q

Structure of cell walls

The cell wall is a … that ___ the cell.

… cells all have cell walls.

The major organic molecule in fungal cell walls is ___

Plant and algal cell walls are made of ___

Cellulose is a ___ made up of glucose units.

A

Structure of cell walls

The cell wall is a rigid covering that protects the cell.

Plant, fungal and algal cells all have cell walls.

The major organic molecule in fungal cell walls is chitin.

Plant and algal cell walls are made of cellulose.

Cellulose is a polysaccharide made up of glucose units.

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33
Q

Function of cell walls

The cell wall provides … and gives ___ to the cell.

A

Function of cell walls

The cell wall provides structural support and gives shape to the cell.

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34
Q

Structure of cell vacuoles

Plant cells each have a large … that occupies most of the area of the cell.

These vacuoles are surrounded by a membrane called the ___ and contain a ___ solution of …

Cell vacuoles are found in the cytoplasm of ___ cells but are NOT present in ___ cells.

A

Structure of cell vacuoles

Plant cells each have a large central vacuole that occupies most of the area of the cell.

These vacuoles are surrounded by a membrane called the tonoplast and contain a weak solution of salts and sugars called cell sap.

Cell vacuoles are found in the cytoplasm of plant cells but are NOT present in animal cells.

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35
Q

Function of cell vacuoles

The central vacuole allows the cell to remain ___

When the central vacuole holds more water, the vacuole … and …

This stops the plant from …

The vacuole also isolates … that are unwanted by the rest of the cell.

A

Function of cell vacuoles

The central vacuole allows the cell to remain rigid.

When the central vacuole holds more water, the vacuole pushes against the cell wall and pressure is maintained.

This stops the plant from wilting.

The vacuole also isolates certain chemicals that are unwanted by the rest of the cell.

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36
Q

Prokaryotic Cells

Prokaryotic cells have some features that are also found in eukaryotic cells.

Prokaryotic cells may also contain …

A

Prokaryotic Cells

Prokaryotic cells have some features that are also found in eukaryotic cells.

Prokaryotic cells may also contain plasmids, a capsule and one or more flagella.

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37
Q

Prokaryotes vs eukaryotes

Features common to these types of cells are: (3)

So, the main difference in terms of organelles is that prokaryotes do NOT have …

A

Prokaryotes vs eukaryotes

Features common to these types of cells are:
A plasma membrane.
Cytoplasm.
DNA.
Ribosomes (ribosomes are smaller in prokaryotes).

So, the main difference in terms of organelles is that prokaryotes do NOT have any membrane-bound organelles (e.g. nucleus, mitochondria, Golgi apparatus etc.).

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38
Q

DNA in prokaryotes

Prokaryotic DNA is found as a … in the ___

Some prokaryotes have smaller loops of DNA called ___ that are not part of the …

Bacteria can exchange ___ with other bacteria, sometimes receiving … that the recipient can add to their ..

… is one trait that often spreads through a bacterial colony through plasmid exchange.

A

DNA in prokaryotes

Prokaryotic DNA is found as a circular molecule in the cytoplasm.

Some prokaryotes have smaller loops of DNA called plasmids that are not part of the main circular DNA molecule.

Bacteria can exchange plasmids with other bacteria, sometimes receiving beneficial new genes that the recipient can add to their chromosomal DNA.

Antibiotic resistance is one trait that often spreads through a bacterial colony through plasmid exchange.

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39
Q

Capsule

The capsule enables prokaryotic cells to …

A

Capsule

The capsule enables prokaryotic cells to attach to surfaces in its environment.

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40
Q

Flagella, pili and fimbriae

Some prokaryotes have flagella, pili, or fimbriae:

Flagella are tail-like and used for locomotion.

Some prokaryotes have none and others have more than one.

Pili are used to exchange genetic material during a type of reproduction called conjugation.

Fimbriae are used by bacteria to attach to a host cell.

A

Flagella, pili and fimbriae

Some prokaryotes have flagella, pili, or fimbriae:

Flagella are tail-like and used for locomotion.

Some prokaryotes have none and others have more than one.

Pili are used to exchange genetic material during a type of reproduction called conjugation.

Fimbriae are used by bacteria to attach to a host cell.

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41
Q

Binary Fission

Prokaryotes, such as bacteria, replicate by …

For ___ organisms, cell division is the only method to produce new individuals.

A

Binary Fission

Prokaryotes, such as bacteria, replicate by binary fission.

For unicellular organisms, cell division is the only method to produce new individuals.

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42
Q

Binary fission

1) Replication of genetic material

Binary fission in prokaryotic cells involves replication of …

2) Migration of genetic material

The two sets of genetic material migrate towards …

3) Cytoplasm begins to divide

The cytoplasmic contents must be ___ to give both new cells …

4) Formation of daughter cells

Two daughter cells are formed, each with … and …

Daughter cells are genetically ___ to the parent cells and identical to ___

The only difference between the daughter cells is …

A

Binary fission

1) Replication of genetic material

Binary fission in prokaryotic cells involves replication of the circular DNA and of plasmids.

2) Migration of genetic material

The two sets of genetic material migrate towards opposite poles.

3) Cytoplasm begins to divide

The cytoplasmic contents must be divided to give both new cells the machinery to sustain life.

4) Formation of daughter cells

Two daughter cells are formed, each with a single copy of the circular DNA and a variable number of plasmid copies.

Daughter cells are genetically identical to the parent cells and identical to each other.

The only difference between the daughter cells is the number of plasmid copies in each cell.

43
Q

Viruses

Viruses are ___ and ___

Virus particles include …

A

Viruses

Viruses are acellular and non-living.

Virus particles include genetic material, a capsid and attachment proteins.

44
Q

Structure

Viruses are made up of …

The nucleic acids are surrounded by …

Viruses are smaller than ___

A

Structure

Viruses are made up of nucleic acids.

The nucleic acids are surrounded by a protein called a capsid.

Viruses are smaller than bacteria.

45
Q

Living cells

Viruses are NOT considered living because: (3)

A

Living cells

Viruses are NOT considered living because:

They have no nucleus, plasma membrane, cytoplasm or ribosomes.

They are not made of cells.

They cannot reproduce independently.

46
Q

Function

To replicate, viruses have to …

These cells are then termed …

Viruses have … that bind to … on host cells.

They then inject … into the host cell.

The host cell divides as normal and the virus is ___

A

Function

To replicate, viruses have to invade and hijack the reproductive mechanism of a living cell.

These cells are then termed host cells.

Viruses have attachment glycoproteins that bind to complementary receptors on host cells.

They then inject DNA or RNA into the host cell.

The host cell divides as normal and the virus is replicated.

47
Q

Two parameters that are important in microscopy are …

A

Magnification and Resolution

Two parameters that are important in microscopy are magnification and resolution

48
Q

Magnification

Magnification is the process of …

The image size is …

The actual size is…

A

Magnification

Magnification is the process of enlarging an object in appearance.

The image size is how big the object appears to be in a picture or drawing, which will be in milimeters (mm).

The actual size is often given in micrometers (µm) - units must be converted so that they are the same.

49
Q

Calculating magnification

The equation for magnification is:

Magnification =

E.g. A mitochondrion is 20 µm long.
An image of the mitochondrion is measured as 20 mm long. What is the magnification?

Magnification =

A

Calculating magnification

The equation for magnification is:

Magnification = size of image ÷ size of real object

E.g. A mitochondrion is 20 µm long.
An image of the mitochondrion is measured as 20 mm long. What is the magnification?

Magnification = 20,000 µm ÷ 20 µm = 1,000x

50
Q

Resolution

Resolution is the ability of a microscope to…

The higher the resolution, …

A

Resolution

Resolution is the ability of a microscope to distinguish two adjacent structures as separate.

The higher the resolution, the better the clarity and detail of the image.

51
Q

Cell Fractionation

Cell fractionation separates …

The steps involved are:

1) Homogenisation

The tissue sample is …

The tissue sample must be kept in specific conditions:

Ice cold (…).

Isotonic solution (...).
And also so no ...

Buffered solution (…).

2) Filtration

The tissue sample is …

The gauze separates …

The organelles are filtered into …

3) Ultracentrifugation

The samples are …

Each tube must be …

Centrifugation separates the sample into ____

Heavier organelles are …

Lighter organelles …

… (e.g. cell walls) forms … leaving the ___ (a liquid) above it that contains the organelles.

4) Ultracentrifugation

The ___ is ___ off and ___ at a … to separate the next ___ organelles (the nuclei).

This is ___ at … to separate each fraction

5) Order of fractionation

The process of … produces fractions of cell organelles from heaviest to lightest.

This order is :

A

Cell Fractionation

Cell fractionation separates organelles according to size to allow them to be studied in an electron microscope.

The steps involved are:

1) Homogenisation

The tissue sample is homogenised using a blender to break the cells.

The tissue sample must be kept in specific conditions:

Ice cold (reduces enzyme activity that might damage organelles).

Isotonic solution (prevents osmosis that could shrink or burst organelles).

No osmosis takes place in isotonic solution.

Buffered solution (keeps pH constant and 
avoids damaging the protein structures).

2) Filtration

The tissue sample is filtered into tubes through a gauze.

The gauze separates larger components from the small, organelles.

The organelles are filtered into tubes to be fractionated using ultracentrifugation.

3) Ultracentrifugation

The samples are spun at a low speed in a centrifuge.

Each tube must be balanced with another tube directly opposite for the centrifuge to work properly.

Centrifugation separates the sample into fractions.

Heavier organelles are forced to the bottom of the tube.

Lighter organelles move towards the top.

Cell debris (e.g. cell walls) forms a pellet at the bottom of the tube, leaving the supernatant (a liquid) above it that contains the organelles.

4) Ultracentrifugation

The supernatant is poured off and centrifuged at a higher speed to separate the next heaviest organelles (the nuclei).

This is repeated at increasingly higher speeds to separate each fraction.

5) Order of fractionation

The process of repeated ultracentrifugation produces fractions of cell organelles from heaviest to lightest.

This order is:
Nucleus.
Chloroplasts.
Mitochondria.
Lysosomes.
Endoplasmic reticulum.
Ribosomes.
52
Q

What are the steps involved in cell fractionation?

1
The tissue sample is …

2
The tissue sample is ___ into …

3
The ____ are ___ into …

4
The samples are ____ at a …

5
The ____ is ____ off and ___ at a ___ speed to …
This is ____ at ___ ___ speeds to separate …

A

What are the steps involved in cell fractionation?

1
The tissue sample is homogenised using a blender to break the cells.

2
The tissue sample is filtered into tubes through a gauze to separate larger components from the small organelles.

3
The organelles are filtered into tubes to be fractionated using ultracentrifugation.

4
The samples are spun at a low speed in a centrifuge to separate the sample into fractions.

5
The supernatant is poured off and centrifuged at a higher speed to separate the next heaviest organelles.
This is repeated at increasingly higher speeds to separate each fraction.

53
Q

Optical (light) microscopes

… passes and is bent through the lens system to ..

The specimen can be ____

Individual cells are generally ____ and their components are not ____ unless they are coloured with …

____ usually kills the cells.

A

Optical (light) microscopes

Visible light passes and is bent through the lens system to enable the user to see the specimen.

The specimen can be alive.

Individual cells are generally transparent and their components are not distinguishable unless they are coloured with special stains.

Staining usually kills the cells.

54
Q

Uses of light microscopes

Most student microscopes are classified as …

Maximum resolution is …

… can be seen with a light microscope.

The maximum magnification is around ___

A

Uses of light microscopes

Most student microscopes are classified as light microscopes.

Maximum resolution is 0.2 micrometres.

The nucleus and mitochondria can be seen with a light microscope.

The maximum magnification is around x1,500.

55
Q

Electron microscopes

In contrast to light microscopes, electron microscopes use …

This allows …

This means that …

A

Electron microscopes

In contrast to light microscopes, electron microscopes use a beam of electrons instead of a beam of light.

This allows higher magnification and higher resolving power.

This means that more detail can be seen.

56
Q

Uses of electron microscopes

Electron microscopes have a maximum resolution of …

This is around ___ times more than light microscopes.

The maximum magnification is around ___

A

Uses of electron microscopes

Electron microscopes have a maximum resolution of 0.0002 micrometres.

This is around 1000 times more than light microscopes.

The maximum magnification is around x1,500,000.

57
Q

Types of Electron Microscopes

There are two main types of electron microscopes:

A

Types of Electron Microscopes
There are two main types of electron microscopes:

transmission (TEM) and scanning (SEM) electron microscopes.

58
Q

TEM

In a TEM, the electron beam …

TEMs use ___ to …

TEMs are …

In thin specimens, you can see the internal structures of organelles such as ___

A

TEM

In a TEM, the electron beam penetrates the cell and provides details of a cell’s internal structures.

TEMs use electromagnets to focus the electron beam.

TEMs are high resolution microscopes.

In thin specimens, you can see the internal structures of organelles such as chloroplasts.

59
Q

SEM

In a SEM, a beam of electrons …, creating details of …

SEMs knock ____ off the specimen and these electrons … to form an ___

SEM images can be ___

Specimens do NOT have to be thin like when using a ___

Resolution is ___ than that produced by a TEM.

A

SEM

In a SEM, a beam of electrons moves back and forth across a cell’s surface, creating details of cell surface characteristics.

SEMs knock electrons off the specimen and these electrons come together to form an image.

SEM images can be three-dimensional.

Specimens do NOT have to be thin like when using a TEM.

Resolution is lower than that produced by a TEM.

60
Q

What is the name of the fluid-filled membrane sacs found within the chloroplasts?

A

What is the name of the fluid-filled membrane sacs found within the chloroplasts?

Thylakoids

61
Q

Cell Division

Viruses require a … to ___

Cells that have the ability to divide have a …

A

Cell Division

Viruses require a host cell to divide.

Cells that have the ability to divide have a cell cycle.

62
Q

Phases of the cell cycle

The cell cycle has two major phases:

During ___, the cell …

During the …, the …

A

Phases of the cell cycle

The cell cycle has two major phases:

interphase and the mitotic phase.

During interphase, the cell grows and DNA is replicated.

During the mitotic phase, the replicated DNA and cytoplasmic contents are separated, and the cell divides.

63
Q

Interphase

Interphase is subdivided into three growth stages called ___, ___ and ___ stage.

In ___, the cell …

In ___, … happens

In ___, the …

A

Interphase

Interphase is subdivided into three growth stages called G1, S and G2 stage.

In G1, the cell grows.

In S, DNA synthesis happens.

In G2, the cell grows some more before the mitotic phases begins.

64
Q

The mitotic phase

Mitosis is the part of the cell cycle in which …

___ organisms go through mitosis to …

A

The mitotic phase

Mitosis is the part of the cell cycle in which a eukaryotic cell divides to produce two daughter cells, each with the identical copies of DNA produced by the parent cell during DNA replication.

Multicellular organisms go through mitosis to grow and repair damaged tissues.

65
Q

Stages of Mitosis

In mitosis, chromosomes go through …

A

Stages of Mitosis

In mitosis, chromosomes go through interphase, prophase, metaphase, anaphase and telophase.

66
Q

Stages of mitosis

Interphase

The cell prepares to ___

DNA is ___ by …

There are now …

The ___ are also replicated.

More ___ is produced to be used in cell division.

A

Stages of mitosis

Interphase

The cell prepares to divide.

DNA is replicated by semi-conservative replication.

There are now two copies of every chromosome.

The organelles are also replicated.

More ATP is produced to be used in cell division.

67
Q

Stages of mitosis

Prophase

The nuclear envelope …

Chromosomes are …

The chromosomes …

They can be seen under a …

Small … called centrioles move to …

___ form the … between the centrioles.

A

Stages of mitosis

Prophase

The nuclear envelope breaks down and the nucleolus disappears.

Chromosomes are left floating in the cytoplasm.

The chromosomes coil more tightly and become shorter and fatter.

They can be seen under a light microscope.

Small protein bundles called centrioles move to opposite poles of the cell.

Microtubules form the mitotic spindle between the centrioles.

68
Q

Stages of mitosis

Metaphase

The chromosomes …

In Metaphase, the chromosomes are …

They are attached to …

A

Stages of mitosis

Metaphase

The chromosomes line up along the mid-line of the cell.

In Metaphase, the chromosomes are Maximally condensed.

They are attached to the spindle by the centromere.

69
Q

Stages of mitosis

Anaphase

The chromosomes …

The sister …

The spindles ___ and …

A

Stages of mitosis

Anaphase

The chromosomes break into two chromatids.

The sister chromatids separate at the centromere.

The spindles contract and pull the chromatids to each pole of the cell.

70
Q

Stages of mitosis

Telophase

The chromatids reach the …

Nuclear envelopes form around the ____ so there are now …

The ____ splits and … are formed.

The daughter cells are identical to …

The cell cycle repeats.

A

Stages of mitosis

Telophase

The chromatids reach the opposite poles and begin to decondense (unravel), becoming chromosomes again.

Nuclear envelopes form around the chromosomes so there are now two nuclei.

The cytoplasm splits and two daughter cells are formed.

The daughter cells are identical to the original cell and to each other.

The cell cycle starts again.

71
Q

Way to remember the stages:

\_\_\_ (interphase).
\_\_\_ (prophase).
\_\_\_ (metaphase).
\_\_\_ (anaphase).
\_\_\_ (telophase).
A

Way to remember the stages:

I (interphase).
Picked (prophase).
My (metaphase).
Apples (anaphase).
Today (telophase).
72
Q

Centromere

The …

A

Centromere

The central region of a chromosome to which the microtubules of the spindle attach.

73
Q

Chromatids

Each contains a …

A

Chromatids

The two thread-like strands into which a chromosome divides longitudinally during cell division.

Each contains a double helix of DNA.

74
Q

Centriole

A … located near …

involved in the …

A

Centriole

A pair of small cylindrical organelles located near the nucleus

involved in the development of spindle fibres in cell division.

75
Q

1) Sample preparation

Wear ___ and use ___ to handle the tips.

Root tips must be ___ (actively growing).

Place into …

After __ minutes, rinse …

A

1) Sample preparation

Wear gloves and use forceps to handle the tips.

Root tips must be sprouting (actively growing).

Place into 5 M hydrochloric acid.

After 5 minutes, rinse the tips in cold water in a watch glass.

76
Q

2) Cut the root tips

Using a …, cut root tips that are …

Place a root tip onto …

Ensure the slide is ___ to reduce the chances of ___

A

2) Cut the root tips

Using a sharp scalpel, cut root tips that are 2 mm long.

Place a root tip onto a microscope slide.

Ensure the slide is clean to reduce the chances of artefacts.

77
Q

3) Staining

Carefully add … and …

Use a ___ needle to …

Place a ___ over the top of the ___

A

3) Staining

Carefully add 2-3 drops of stain and leave for two minutes.

Use a mounted needle to spread out the root tips into a thin layer.

Place a coverslip over the top of the tips.

78
Q

4) Squashing

Squash down by …

This could be with the flat end of a pencil, or …

Force must be ___ or the cover slip may ___ and cause ___

A

4) Squashing

Squash down by applying force to the cover slip.

This could be with the flat end of a pencil, or the slide could be covered with a paper towel and pressed.

Force must be vertical or the cover slip may break and cause injury.

79
Q

5) Viewing the sample

Place the slide on the microscope stage using the …

Focus the lens on the sample using first the …

Move the slide to …

The cells closer to the tip will be those more …

On a lens power of ___, it should be possible to clearly see the …

A

5) Viewing the sample

Place the slide on the microscope stage using the lowest power lens.

Focus the lens on the sample using first the coarse control and then the fine control.

Move the slide to see the range of cells.

The cells closer to the tip will be those more actively dividing.

On a lens power of 400x, it should be possible to clearly see the chromosomes in the dividing cells.

80
Q

The equation for mitotic index for a particular field of view is:

Mitotic index = …

A

The equation for mitotic index for a particular field of view is:

Mitotic index = number of cells in mitosis ÷ total number of cells

81
Q

In a field of view, 29 cells are counted in total.

22 of the 29 cells have visible chromosomes and are actively dividing.

About 76% of the cells in the sample are undergoing mitosis.

A

In a field of view, 29 cells are counted in total.

22 of the 29 cells have visible chromosomes and are actively dividing.

22 ÷ 29 = 0.7586

About 76% of the cells in the sample are undergoing mitosis.

82
Q

What is used to work out the proportion of cells in the sample that are in mitosis?

A

What is used to work out the proportion of cells in the sample that are in mitosis?

MITOTIC INDEX

83
Q

The darker the solution of beetroot, the ….

A

The darker the solution of beetroot, the more pigment has been released because the membrane is more permeable. A darker solution will have a higher reading for absorbance.

84
Q

Steps Involved in Investigating Cell Membrane Permeability

A

Steps Involved in Investigating Cell Membrane Permeability

collect beetroot samples

put them in ethanol solutions

remove the discs and put them in distilled water in a cuvette

calibrate the colorimeter

measure the absorbance of each solution

85
Q

Structures of the cell membrane:

Plasma
membrane

Phospholipid

Cholesterol

A

Structures of the cell membrane:

Plasma
membrane
The structure that defines the borders of cells and most organelles.

Phospholipid
The molecules that form a bilayer in membranes. The molecules are made from glycerol, two fatty acids and phosphate-linked head group.

Cholesterol
Lipid that sits in the core of the membrane.

86
Q

What absorbance is the colorimeter calibrated to?

A

What absorbance is the colorimeter calibrated to?

520 nm

87
Q

1) Collect beetroot samples

Use a cork borer to …
Cut discs of a uniform depth using …
This removes …

A

1) Collect beetroot samples

Use a cork borer to collect samples of uniform diameter.
Cut discs of a uniform depth using a sharp scalpel on a white tile and rinse in cold water.
This removes excess pigment that has leaked through physically broken cell membranes.

88
Q

2) Add ethanol

Prepare …
Place the discs into …
Make sure …

A

2) Add ethanol

Prepare at least five concentrations of ethanol (e.g. 0%, 10%, 20%, 30%, 40%) in beakers.
Place the discs into the corresponding solution for 10 minutes.
Make sure the samples are completely covered by the ethanol solutions and mixed frequently throughout the 10 minutes.

89
Q

3) Remove the discs

Remove the discs from the solutions to …

A

3) Remove the discs

Remove the discs from the solutions to prevent further changes and allow a fair comparison between the experiments.

90
Q

4) Calibrate the colorimeter

Calibrate a …
The cuvettes must be dry and the clear sides must not be touched to prevent potential errors in the readings.

A

4) Calibrate the colorimeter

Calibrate a colorimeter by using a cuvette of distilled water at an absorbance of 520nm.
The cuvettes must be dry and the clear sides must not be touched to prevent potential errors in the readings.

91
Q

5) Measure absorbance

Measure the ….
Plot …
The darker …

A

5) Measure absorbance

Measure the absorbance of each solution.
Plot the results in a graph with concentration on the x-axis and absorbance on the y-axis.
The darker the solution, the more pigment has been released. This is reflected in a higher reading for absorbance.

92
Q

Diffusion

Diffusion describes ….

A

Diffusion

Diffusion describes the passive movement of particles in fluids (liquids and gases).

93
Q

Diffusion

Molecules move randomly but tend to move into any space available until…
So, the net movement of particles will be…
Substances such as … can move in and out of cells across cell membranes via diffusion.

A

Diffusion

Molecules move randomly but tend to move into any space available until it is evenly distributed.
So, the net movement of particles will be towards the area of lower concentration or down a concentration gradient.
Substances such as CO2, O2 and urea can move in and out of cells across cell membranes via diffusion.

94
Q

E.g. Perfume

When you spray perfume, the smell diffuses across the room from …

A

E.g. Perfume

When you spray perfume, the smell diffuses across the room from the area of high concentration (where you just sprayed it) to the area of low concentration (the other side of the room).

95
Q

What do we call the net movement of particles from an area of high concentration to an area of lower concentration?

A

What do we call the net movement of particles from an area of high concentration to an area of lower concentration?

diffusion

96
Q

Large or polar molecules

Some materials are too large to move through the membrane.
Other materials are polar molecules that are repelled by the hydrophobic part of the membrane.
These materials move across the membrane by facilitated diffusion.

A

Large or polar molecules

Some materials are too large to move through the membrane.
Other materials are polar molecules that are repelled by the hydrophobic part of the membrane.
These materials move across the membrane by facilitated diffusion.

97
Q

Steps Involved in Staining Plant Root Tips

A

Steps Involved in Staining Plant Root Tips

prepare the sample

cut the root tips

stain the root tips

squash the root tips

view the sample at 400x power w a light microscope

98
Q

How must force be applied when squashing plant root tips?

A

How must force be applied when squashing plant root tips?

vertically

99
Q

Errors in DNA replication can lead to the development of ____ Drugs can be targeted to ….

A

Errors in DNA replication can lead to the development of tumours. Drugs can be targeted to damage DNA and kill the cells to prevent spread of a tumour.

100
Q

The cell cycle is tightly controlled by genes, however errors do happen. If changes to the DNA nucleotide sequence happen within a coding portion of a gene and are not corrected, … results.

A

The cell cycle is tightly controlled by genes, however errors do happen. If changes to the DNA nucleotide sequence happen within a coding portion of a gene and are not corrected, a gene mutation results.

101
Q

What phase in interphase produces new organelles and causes the cells to grow in size?

A

What phase in interphase produces new organelles and causes the cells to grow in size?

g1

102
Q

What is the normal thickness of the phospholipid bilayer?

A

What is the normal thickness of the phospholipid bilayer?

5-10nm

103
Q

The more easily the phospholipid bilayer is dissolved, the more …

A

The more easily the phospholipid bilayer is dissolved, the more permeable the membrane.

104
Q

the permeability of cell membranes:

A

the permeability of cell membranes:

pH

solvent concentration

temperature