Cells 2.1-2.2 Flashcards

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1
Q

Structure of eukaryotic cells

A
Cell surface membrane 
Nucleus 
Mitochondria
Chloroplasts
Golgi Apparatus and Golgi vesicles 
Lysosomes 
Ribosomes
Rough and smooth endoplasmic reticulum 
Cell wall
Cell vacuole
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2
Q

The nucleus structure

A
Nuclear envelope
Nuclear pores
Nucleoplasm
Chromosomes
Nucleolus
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3
Q

Nucleus’ functions

A

Act as the control centre of the cells through the production of mRNA and tRNA and hence protein synthesis
Retain the genetic material of the cell in the form of DNA and chromosomes
Manufacture ribosomal RNA and ribosomes

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4
Q

Nuclear envelope

A

A double membrane that surrounds the nucleus. It’s outer membrane is continuous with the endoplasmic reticulum of the cell and often has ribosomes on the surface. It controls the entry and exit of materials in and out of the nucleus and contains the reactions taking place within it

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5
Q

Nuclear pores

A

Allow the passage of large molecules, such as messenger RNA, out of the nucleus. There are typically around 3000 pores in each nucleus

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6
Q

Nucleoplasm

A

The granular, jellylike material that makes up the bulk of the nucleus

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7
Q

Chromosomes

A

Consist of protein-bound, linear DNA

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8
Q

Nucleolus

A

A small spherical region within the nucleoplasm. It manufactures ribosomal RNA and assembles the ribosomes. There may be more than one nucleolus in the nucleus

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9
Q

mitochondria’s structure

A

Double membrane
Cristae
Matrix

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10
Q

Mitochondria’s double membrane

A

Around the organelle is a double membrane that controls the entry and exit of material. The inner of the two membranes is folded to form extensions known as Cristae

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11
Q

Cristae

A

Are extensions of the inner membrane, which in some species extend across the whole width of the mitochondrion. These provide a large surface area for the attachment of enzymes and other proteins involved in respiration

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12
Q

Matrix

A

The matrix makes up the remainder of the mitochondrion. It contains protein, lipids, ribosomes and DNA that allows the mitochondria to control the production of some of their own proteins. Many enzymes involved in respiration are found in the matrix

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13
Q

Mitochondria’s function

A

Mitochondria are the sites of the aerobic stages of respiration
They are therefore responsible for the production of the energy carrier molecule, ATP, from respiratory substrates such as glucose. Because of this, the number and size of mitochondria, and the number of the Cristae, are high in cells that have a high level of metabolic activity and therefore require a plentiful supply of ATP. Examples of metabolically active cells include muscle and epithelial cells.

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14
Q

Chloroplasts structure

A
The chloroplast envelope
The grana
Thylakoids
Chlorophyll
The stroma
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15
Q

The chloroplast Envelope

A

A double plasma membrane that surrounds the organelle

It is highly selective in what it allows to enter and leave the chloroplast

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16
Q

The grana

A

Are stacks of up to 100 disc like structures called thylakoids
Within the thylakoids is the photosynthetic pigment called chlorophyll
Some thylakoids have tubular extensions that join up with thylakoids in adjacent grana
Grana are where the first stage of photosynthesis (light absorption) takes place

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17
Q

The stroma

A

A fluid filled matrix where the second stage of photosynthesis (synthesis of sugars) takes place
Within the stroma are a number of other structures, such as grains

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18
Q

Chloroplast adaptions for function of harvesting sunlight and carrying out photosynthesis

A

the granal membranes provide a large surface area for the attachment of chlorophyll, electron carriers and enzymes that carry out the first stage of photosynthesis. These chemicals attach the membrane in a highly ordered fashion
The fluid of the stroma possesses all the enzymes needed to make sugars in the second stage of photosynthesis
Chloroplasts contain both DNA and ribosomes so they can quickly and easily manufacture some of the proteins needed for photosynthesis

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19
Q

Rough endoplasmic reticulum

A

Has ribosomes present on the outer surfaces of the membranes.

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20
Q

Endoplasmic reticulum

A

An elaborate, three-dimensional system of sheet-like membranes, spreading through the cytoplasm of the cells
It is continuous with the outer nuclear membrane. The membranes enclose a network of tubules and flattened sacs called cisternae. There are two types of ER

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21
Q

Smooth endoplasmic reticulum

A

Lacks ribosomes on its surface and is often more tubular in appearance

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22
Q

RER functions

A

Provide a large surface area for the synthesis of proteins and glycoproteins
Provide a new pathway for the transport of materials, especially proteins, throughout the cell

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23
Q

SER functions

A

Synthesise, store and transport lipids

Synthesise, store and transport carbohydrates

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24
Q

Golgi apparatus function

A

Add carbohydrate to proteins to form glycoproteins
Produce Secretary enzymes, such as those secreted by the pancreas
Secrete carbohydrates, such as those used in making cells walls in plants
Transport, modify and store lipids
Form lysosomes

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25
Q

Golgi apparatus sequence

A

The proteins and lipids produced by the ER are passed through the Golgi apparatus in strict sequence. The Golgi modifies proteins often adding nonprotein components such as carbohydrates to them
It also ‘labels’ them, allowing them to be accurately sorted and sent to the correct destinations
Once sorted, modified proteins and lipids are transported in Golgi vesicles

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26
Q

Lysosomes functions

A

Hydrolyse material ingested by phagocytic cells, such as white blood cells and bacteria
Release enzymes to the outside of the cell in order to destroy material around the cell
Digest worn out organelles so that the useful chemicals they are made of can be reused
Completely break down cells after they have died

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27
Q

How are lysosomes formed

A

Formed when the vesicles produced by the Golgi apparatus contain enzymes such as proteases and lipases
Lysosomes are a type of Golgi vesicle that releases lysozymes

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28
Q

Ribosomes structure

A

Small cytoplasmic granules found in all cells
They may occur in the cytoplasm or be associated with the RER
Have two subunits – one large and one small – each of which contains ribosomal RNA and protein

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29
Q

Two types of ribosomes

A

80S – found in eukaryotic cells, is around 25 nm in diameter

70 S – found in prokaryotic cells, mitochondria and chloroplasts, is slightly smaller

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30
Q

Ribosomes function

A

Despite the small size, they are quite in such fast numbers that they can account for up to 25% of the dry mass of the cell
The site protein synthesis

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31
Q

Cell wall features

A

Consist of a number of polysaccharides, such as Celulose
There is a thin layer called the middle lamella, which marks the boundary between adjacent cells and cements adjacent cells together

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32
Q

Cell wall functions

A

Provide mechanical strength in order to prevent the cell busting under the pressure created by the osmotic entry of water
To give mechanical strength to the plant as a whole
To allow water to pass along it and so contribute to the movement of water through the plants

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33
Q

Vacuoles functions

A

Support herbaceous plants, and herbaceous parts of woody plants, making cells turgid
Sugars and amino acids may act as a temporary food store
The pigments may colour petals to attract pollinating insects

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34
Q

Vacuoles structure

A

Fluid filled sac bounded by a single membrane
Within mature plant cells there is usually one large central vacuole
The single membrane around it is called the tonoplast
A plant vacuole contains a solution of mineral salts, sugars, amino acids, wastes and sometimes pigments

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35
Q

specialised cells

A

In complex multi cellular organisms, eukaryotic cells become specialised for specific functions
Specialised cells are organised into tissues, tissues into organs and organs into systems

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36
Q

Tissues

A

A collection of similar cells that perform a specific function are known as a tissue
Examples of tissues include
Epithelials tissues
Xylem

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37
Q

Epithelial tissues

A

Found in animals and consist of sheets of cells
They line the surface of organs and often have a protective or secretary function
There are many similar types, including those made up of thin, flat cells that line organs where diffusion takes place, for example the alveoli of the lungs, and ciliated epithelium that lines a duct such as the trachea
The cilia are used to move the mucus over the epithelial surface

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38
Q

Xylem

A

Occurs in plants and is made up of a number of similar cell types
It is used to transport water and mineral ions throughout the plant and also gives mechanical support

39
Q

Organs

A

An organ is a combination of tissues that are coordinated to perform a variety of functions, although they often have one predominant major function
In animals for example, the stomach is an organ that is involved in the digestions of certain types of food
It is made up of tissues such as:
Muscles to churn and mix the stomach contents
Epithelium to protect the stomach wall and produce secretions
Connective tissue to hold together the other tissues

40
Q

Organ systems

A

Organs work together as a single unit known as an organ system
The systems may be grouped together to perform particular functions more efficiently
There are number of organ systems in humans
The digestive system
The Respiratory system
The circulatory system

41
Q

How do prokaryotic cells differ from eukaryotic cells

A

Prokaryotic cells are much smaller
Have cytoplasm that lacks membrane-bound organelles
Have smaller ribosomes
Have no nucleus; instead they have a singular DNA molecule that is free in the cytoplasm and is not associated with proteins
Have a cell wall that contains murein, a glycoprotein
They have one or more plasmids whereas eukaryotic cells have none
They have a capsule surrounding the cell
They have one or more flagella

42
Q

Capsule role (prokaryotic cell)

A

Protects bacteria from other cells and helps groups of bacteria to stick together for further protection

43
Q

Circular DNA role (prokaryotic cells)

A

Possesses the genetic information for the replication of bacterial cells

44
Q

Plasmid role (prokaryotic cells)

A

Possesses the genes that may aid the survival of bacteria in adverse conditions, e.g. produces enzymes that break down antibiotics

45
Q

Viruses

A

They are acellular, nonliving particles
They are smaller than bacteria
They contain nucleic acids such as DNA or RNA as genetic material but can only multiply inside living host cells
The Nucleic acid is enclosed within a protein coat called the capsid
Some viruses, like the human in immunodeficiency virus, are further surrounded by a lipid envelope. The Lipid envelope, or if this is not present, the Capsid, have attachment proteins which are essential to allow the virus to identify and attach to a host cell

46
Q

2 types of microscope

A

Light and Electron (transmission and scanning)

47
Q

Which microscope has highest magnification

A

TEM > SEM > LM
x12,000,000
x200,000
x2,000

48
Q

Which microscope has higher resolution

A

TEM > SEM > LM
0.1nm
20nm
200nm

49
Q

Resolution

A

minimum distance at which 2 very close objects can be distinguished

50
Q

1mm=?um

A

1000um

51
Q

1mm=?nm

A

1,000,000 nm

52
Q

Magnification formula

A

magnification = image size/actual size

53
Q

Why does electron microscopes have a higher resolution?

A

Electron microscope uses electrons ​which have a shorter wavelength (light microscope uses light which has a large wavelength)

54
Q

Difference between TEM and SEM?

A

in Transmission the electrons pass through the specimen, in Scanning the electrons bounce off the specimen’s surface

55
Q

Advantages and disadvantages of TEM

A

− Advantage = highest magnification and highest resolution
− Disadvantage = works in a vacuum so can only observe dead specimens, specimen needs to be thin, black and white image, 2D image, image may contain artefacts, complicated staining process is required and can build up 3D image but is slow and complicated process

56
Q

What are artefacts

A

Things that result from the way the specimen is prepared
Artefacts may appear on the finished photomicrograph but are not part of the natural specimen
It is therefore not always easy to be sure that what we see on the photomicrograph really exists in that form

57
Q

Advantages and disadvantages of SEM

A

− Advantage = produces 3D image, doesn’t need to be thin specimen as electrons don’t penetrate
− Disadvantage = works in a vacuum so can only observe dead specimens, black and white image, artefacts, complex staining process,

58
Q

Cell fractionation

A

Process where cells are broken up and different organelles they contain are separated out

59
Q

Cell fractionation process

A

Tissues placed in cold, buffer solution of the same water potential as the tissue:
Homogenation: cells are broken up by homogeniser (blender). Releases organelles from cell. Resultant fluid (homogenate) is then filtered to remove any complete cells and large pieces of debris
Ultracentrifugation: where fragments are separated in a centrifuge whuc spins filtrate at high speed in the following way; tube of filtrate spun at low speed which forces the heaviest organelles (nuclei) to the bottom where they form a pellet or sentiment; fluid (supernatant) at the top is removed and placed in another tube which is spun at a faster speed which removes the next heaviest organelle (mitochondria). this process is repeated for each of the organelles

60
Q

Cell fractionation required conditions

A

– COLD buffer solution – prevents enzyme activity that might break down organelle
– same water potential – prevent organelles from bursting/shrinking
– buffered – pH doesn’t fluctuate (alters structure

61
Q

Scientific community and artefacts

A

There was a considerable period of time during which the scientific community distinguished between artefacts and cell organelles

62
Q

By what processes can cell division take place

A

Mitosis or meiosis

63
Q

Mitosis versus meiosis

A

Mitosis – produces two daughter cells that have the same number of chromosomes as the parent cell and each other
Meiosis – produces four daughter cells, each with half the number of chromosomes as the parent cell

64
Q

Mitosis

A

Division of a cell results in each of the daughter cells having an exact copy of the DNA of the parent cell
Except in the rare event of the mutation, the genetic make up of two daughter nuclei is also identical to that of the parent nucleus
Mitosis is always preceded by the period during which the cell is not dividing. This period is called interphase. Its is a period of considerable cellular activity that includes a very important event, the replication of DNA
The two copies of DNA after replication remain joined at a place called the centromere.
Although mitosis is a continuous process it can be divided into four stages convenience:
Prophase, metaphase, anaphase and telophase

65
Q

Three stages of interphase

A

G1– first growth phase- longest phase
S – replication phase
G 2 –second growth phase – short

66
Q

G1-first growth phase

A

Protein synthesis – cell grows
Most organelles produced
Volume of cytoplasm increases
Cell differentiation (switching on or off of genes)
Length depends on internal and external factors
If cell is not going to divide again it remains in this phase
DNA content = 20

67
Q

S – replication phase

A

DNA replication – this must occur if mitosis is to take place
The cell enters this phase only if cell division is to follow
DNA content = 40

68
Q

G2 – second growth phase

A

Short gap before mitosis (cell division)
Cytoskeleton of cell breaks down and the protein microtubule components begin to reassemble into spindle fibres – required for cell division
DNA content = 40

69
Q

4 mitotic phases

A
Prophase
Metaphase
Anaphase
Telophase 
(I) punched my annoying teacher
70
Q

Eukaryotic cells that do retain the ability to divide show:

A

A cell cycle

71
Q

When does DNA replication occur in the cell cycle

A

Interphase

72
Q

Prophase

A

The chromosomes first become visible, initially as long thin fibres, which later shorten and thicken. Animal cells contain two cylindrical organelles called centrioles, each of which moved to opposite ends (called Poles) of the cell
From each of the centrioles, spindle fibres develop from pole to pole (collectively called spindle apparatus)
The nucleolus disappears and the nuclear envelope breaks down, leaving the chromosomes free in the cytoplasm of the cell
These chromosomes are drawn towards the equator of the cell by the spindle fibres attached the centromere

73
Q

Metaphase

A

The chromosomes are seen to be made up of two chromatids
Each chromatid is an identical copy of DNA from the parent cell
The chromatids are joined by the centromere
It is to this centromere that some microtubules from the poles are attached, and the chromosomes are pulled along by the spindle apparatus and arrange themselves across the equator of the cell

74
Q

Anaphase

A

The centromeres divide into two and the spindle fibres pull the individual chromatids making up the chromosomes apart
The chromatids move rapidly to the respective, opposite poles of the cell and we now refer to them as chromosomes
The energy for the process is provided by mitochondria which gather around the spindle fibres
If cells are treated with chemicals that destroy the spindle, the chromosomes remain at the equator, unable to reach the polls

75
Q

Telophase

A

The chromosomes reach their respective poles and become longer and thinner, finally disappearing altogether, leaving only widely spread chromatin
Spindle fibres disintegrate and the nuclear envelope and nucleolus reform

76
Q

What happens after mitosis in the cell cycle

A

Cytokinesis

77
Q

Cytokinesis

A

Process where the cytoplasm divides
Cell divides into two
DNA content = 20

78
Q

Mitosis phases and simple description of each phase

A

Prophase: DNA coils to form chromosomes, nucleus breaksdown, spindle fibres form
​Metaphase: chromosomes line up in middle of cell and attach to spindle fibre via centromere
​Anaphase: spindle fibres pull, centromere splits, sister chromatids move to opposite sides
​Telophase: chromatids uncoil, nucleus reforms (left with 2 genetically identical nuclei)

79
Q

Difference between chromatin, chromosomes and chromatids

A
  • Chromatin is composed of DNA and histones that are packaged into thin, stringy fibers. These chromatin fibers are not condensed. During cell division, chromatin condenses to form chromosomes.
  • Chromosomes are single-stranded groupings of condensed chromatin. During the cell division processes of mitosis and meiosis, chromosomes replicate to ensure that each new daughter cell receives the correct number of chromosomes. A duplicated chromosome is double-stranded and has the X shape. The two strands are identical and connected in a central region called the centromere.
  • A chromatid is either of the two strands of a replicated chromosome. Chromatids connected by a centromere are called sister chromatids. At the end of cell division, sister chromatids separate, becoming daughter chromosomes in the newly formed daughter cells.
80
Q

Explain the appearance of cells in interphase stage of cell cycle

A

One cell

Genetic material not in chromosomes, in chromatin inside nucleus, all bundled in circular form

81
Q

Explain the appearance of cells in prophase stage of mitosis

A

One cell

Chromosomes are bundled together in the centre circular form (in nucleus)

82
Q

Explain the appearance of cells in metaphase stage of mitosis

A

One large cell

Chromosomes all lined up in centre of cell

83
Q

Explain the appearance of cells in anaphase stage of mitosis

A

One cell

DNA in chromosomes at separate poles of cell

84
Q

Explain the appearance of cells in telophase stage of mitosis

A

One cell
Chromatin in two separate bundles at opposite sides of cell
Doesn’t look like chromosomes – widely spread in circular shapes

85
Q

Uncontrolled cell division

A

Mitosis is a controlled process
Uncontrolled cell division can lead to the formation of tumours and of cancers
Many cancer treatments are directed at controlling the rate of cell division

86
Q

Cell division in prokaryotic cells

A

Binary fission

87
Q

Binary fission process

A

– The circular DNA molecule replicates and both copies attach to the cell membrane
– the plasmids also replicate
– the cell membrane begins to grow between the two DNA molecules and begins to pinch inwards, dividing the cytoplasm in two
– a new cell wall formed between the two molecules of DNA, dividing the original cell into two identical daughter cells, each with a single copy of the circular DNA and variable number of copies of the plasmids

88
Q

Why don’t viruses undergo cell division?

A

They are nonliving

89
Q

Replication of viruses

A

They replicate by attaching to the host cell with the attachment proteins on the surface
They then inject their nucleic acid into the host cell
The genetic information on the injected viral nucleic acid then provides the instructions for the host cell’s metabolic processes to start producing the viral components, nucleic acid, enzymes and structural proteins, which are then assembled into new viruses

90
Q

What is mitosis needed for

A

Growth
Repair
Reproduction (asexual)

91
Q

Cells that don’t divide continually

A

Still undergo a regular cycle of division separated by periods of cell growth
This is known as the cell cycle and has three stages:
Interphase – which occupies most of the cell cycle, and is sometimes known as the resting phase because no division takes place
Nuclear division – when the nucleus divides either in two (mitosis) or four (meiosis)
Division of the cytoplasm – the process by which the cytoplasm divides to produce two new cells or four new cells

92
Q

Cancer

A

cancer cells are rapidly dividing cells (like hair cells, skin cells, red blood cells), they spend less time in interphase and more time in the other stages (mitosis)

93
Q

Treatments for cancer

A

Surgery = aim is to remove tumour

​Chemotherapy = - using drugs that inhibit mitosis in rapidly dividing cancer cells
​ - problem, also affect normal healthy cells (hair cell, skin cells, rbcs) ​ ​ causing side effects (hair loss, dry skin, tiredness)
​ - treatment given as regular doses to allow time for normal healthy ​ ​ cells to recover in number

​Radiotherapy = radiation used to destroy cancer cells