Cell Structure (Chapter 4) Flashcards
Robert Hooke
first scientist to see cell walls with microscope in 1665
light microscope
visible light is passed through the specimen and then glass lens
lenses refact the light to magnify the image
maximum magnification is ~1000x
maximum resolution is about 0.2 micrometer
electron microscope
developed in the 1950s
focuses beam of electrons through specimen or on its surface
100x better than light microscope
transmission electron microscope (TEM)
aims electron beam through thin section of a specimen
specimen is stained with atoms of a heavy metals
electrons passing through are scattered more in dense areas
scanning electron microscope (SEM)
electron beam scans the surface of the sample
beam excites the electrons on the surface
secondary electrons are detected by a device
cell fractionalization
process of placing broken up cells in centrifuge
largest cell components settle to the bottom
enables researchers to use biochemical tests on specific cell parts and organelles
features common to all cells
plasma membrane: selective barrier
cytosol: semifluid, jellylike substance
chromosomes: carry genes in the form of DNA
ribosomes: tiny complexes that make proteins
eukaryotic cell characteristics
DNA in a nucleus with a double membrane
cytoplasm is the only region between nucleus and membrane
variety of organelles
tend to be larger
prokaryotic cell characteristics
DNA concentrated in nucleoid not bounded by membrane
factors affecting cell size
requirement for cellular metabolism sets lower bound on cell size
ratio of surface area to volume of cell sets upper bound on cell
volume grows proportionally bigger than surface area
composition of membranes
double layer of phospholipids and other lipids
proteins are embedded in lipid bilayer
lipids and proteins are suited to membrane functions
plasma membrane
membrane enclosing the cell
cytoskeleton
reinforces cell’s shape
functions in cell movement
components are made of protein
types are microfilaments, intermediate filaments, microtubules
flagellum
motility structure present in some animal cells
composed of a cluster of microtubules within an extension of the plasma membrane
nuclear envelope
double membrane enclosing the nucleus
each membrane is a lipid bilayer with associated proteins
seperated by a space of 20-40 nm
perforated by pores that are 100 nm in diameter
continuous with the ER
nucleolus
non-membranous structure involved in production of ribosomes
ribosomal RNA (rRNA) is synthesized
proteins from cytoplasm assembed with rRNA into ribosomes
nucleus has one or more nucleoli
chromatin
material consisting of DNA and proteins
appears as diffuse mass
visible in a dividing cell as individual condensed chromosomes
ribosomes
complexes that synthesize proteins
free in cytosol or bound to rough ER or nuclear envelope
bound ribosomes make proteins for:
insertion in membranes
packaging within certain organelles such as lysosomes
export from the cell (secretion)
golgi apparatus
organelle active in synthesis, modification, sorting, and secretion of cell products
consists of flattened membranous sacs called cisternae
golgi stack has distinct structural directionality: cis face receives, trans face ships
manufactures some macromolecules
lysosome
digestive organelle where macromolecules are hydrolyzed
acidic environment that is not ctive in cytosol
only in animal cells
mitochondrion
organelle where cellular respiration occurs and most ATP is generated
peroxisome
organelle with various specialized metabolic functions
produces hydrogen peroxide as a by-product then converts it to water
microvilli
projections that increase the cell’s surface area
centrosome
region where the cell’s microtubules are initiated
contains a pair of centrioles
only in animal cells