Cell Structure Flashcards
What’s magnification?
The number of times larger an image appears, compared with the size of the object. Produce linear magnifications. X100 image is 100 times wider and 100 times longer than the original object
What’s an organelle?
Small structures within cells, each of which has a specific function
What’s a photomicrograph?
Photograph of an image seen using an optical microscope
What’s resolution?
The clarity of an image. The ability of an optical instrument to see or produce an image that shows fine detail clearly
Why are optical microscopes still used?
Relatively cheap
Easy to use
Portable and able to be used in the field as well as labs
Able to be used to study while living specimens
What magnification do optical microscopes allow?
Up to x1500
What’s an electron micrograph?
Photograph of an image seen using an electron microscope
Why is an optical microscopes resolution low?
Uses visible light which is part of the electromagnetic spectrum. It has a wavelength between 400 and 700 nm therefore structures closer than 200 nm will appear as one object
How to set up a optical microscope?
Place slide with specimen on the stage and clip into place
Rotate the nosepiece so lowest power lens is over slide.
Adjust the coarse focus know while looking into the eyepiece, until you see a clear image
Whilst viewing, adjust the iris diaphragm for optimum light
Rotate the nosepiece and bring x10 objective into place over the specimen. Look down the ocular tube and use knob to focus the image
How to calculate magnification?
Total magnification= magnifying power of objective lens x magnifying power of the eyepiece lens
About laser scanning microscopes?
Use laser light to scan object point by point, assembled by computer into one image
High resolution and high contrast
Depth selectivity and can focus on structures at different depths with specimens
Can observe while living specimens
Used in medical profession and biological research
What’s the wavelength of electron microscopes?
0.004nm
How do electron microscopes work?
Electrons fired from a cathode and focused by magnets rather than glass lenses on to a screen or photographic plate
About the transmission electron microscope?
Specimen has to be chemically fixed by being dehydrated and stained
Beam of electrons pass through specimens. Some pass through and are focused onto screen
Form 2D black image
Magnification of x2000000
About the scanning electron microscope?
Electrons don’t pass through specimen.
Cause secondary electrons to bounce off the specimens surface and be focused on to a screen
3D image with magnification up to x200000
Black and white
Specimen has to be in vacuum
Coated with fine film of metal
Draw backs of an electron microscope?
Large and very expensive
Need a great deal of skill and training
Specimens have to be dead
Metallic salt stains may be potentially hazardous to the user
What’s the wavelength of electron microscopes?
0.004nm
How do electron microscopes work?
Electrons fired from a cathode and focused by magnets rather than glass lenses on to a screen or photographic plate
About the transmission electron microscope?
Specimen has to be chemically fixed by being dehydrated and stained
Beam of electrons pass through specimens. Some pass through and are focused onto screen
Form 2D black image
Magnification of x2000000
About the scanning electron microscope?
Electrons don’t pass through specimen.
Cause secondary electrons to bounce off the specimens surface and be focused on to a screen
3D image with magnification up to x200000
Black and white
Specimen has to be in vacuum
Coated with fine film of metal
Draw backs of an electron microscope?
Large and very expensive
Need a great deal of skill and training
Specimens have to be dead
Metallic salt stains may be potentially hazardous to the user
Examples of specimens that can be viewed by an optical microscope?
Living organisms such as Amoeba
Smear preparations or human blood and cheek cells
Thin sections of animals, plant and fungal tissue
Observing unstained specimens
Some organisms are colourless or transparent. Some microscopes use light interference, rather than light absorption, in order to produce a clear image without staining
Some use a dark background
Why stain specimens?
Coloured chemicals that bind to molecules on or in the specimen. Some stains bind to specific structures so they can be easily identified
This is called differential staining
Iodine stains cellulose yellow and starch granules blue/black