Cell Structure Flashcards
Biologists use microscopes and the tools of biochemistry to study cells. Eukaryotic cells have internal membranes that compartmentalize their functions. The eukaryotic cell's genetic instructions are housed in the nucleus and carried out by the ribosomes. The endomembrane system regulates protein traffic and performs metabolic functions in the cell. Mitochondria and chloroplasts change energy from one form to another.
Light Microscope (LM)
An optical instrument with lenses that refract (bend) visible light to magnify images of specimens
Organelle
Any of several membrane-enclosed structures with specialized functions, suspended in the cytosol of eukaryotic cells
Electron Microscope (EM)
A microscope that uses magnets to focus an electron beam on or through a specimen, resulting in a practical resolution that is 100-fold greater than that of a light microscope using standard techniques. A transmission electron microscope is used to study the internal structure of thin sections of cells. A scanning electron microscope is used to study the fine detail of cell surfaces
Scanning Electron Microscope (SEM)
A microscope that uses an electron beam to scan the surface of a sample, coated with metal atoms, to study the details of its topography
Transmission Electron Microscope (TEM)
A microscope that passes an electron beam through very thin sections stained with metal atoms and is primarily used to study the internal ultrastructure of cells
Cell Fractionation
The disruption of a cell and separation of its parts by centrifugation at successively higher speeds
Cytosol
The semifluid portion of the cytoplasm
Eukaryotic Cell
A type of cell with a membrane-enclosed nucleus and membrane-enclosed organelles. Organisms with these cells (protists, plants, fungi, and animals) are called eukaryotes
Prokaryotic Cell
A type of cell lacking a membrane-enclosed nucleus and membrane-enclosed organelles. Organisms with these cells (bacteria and archaea) are called prokaryotes
Nucleoid
A non-membrane-enclosed region in a prokaryotic cell where its chromosome is located
Cytoplasm
The contents of the cell bounded by the plasma membrane; in eukaryotes, the portion exclusive of the nucleus
Plasma Membrane
The membrane at the boundary of every cell that acts as a selective barrier, regulating the cell’s chemical composition
Nucleus
The organelle of a eukaryotic cell that contains the genetic material in the form of chromosomes, made up of chromatin
Nuclear Envelope
In eukaryotic cell, the double membrane that surrounds the nucleus, perforated with pores that regulate traffic with the cytoplasm. The outer membrane is continuous with the endoplasmic reticulum
Nuclear Lamina
A netlike array of protein filaments that lines the inner surface of the nuclear envelope and helps maintain the shape of the nucleus
Chromosome
A cellular structure consisting of one DNA molecule and associated protein molecules. (In some contexts, such as genome sequencing, the term may refer to the DNA alone). A eukaryotic cell typically has multiple, linear chromosomes, which are located int he nucleus. A prokaryotic cell often has a single, circular chromosome, which is found in the nucleoid, a region that is not enclosed by a membrane
Chromatin
The complex of DNA and proteins that make up eukaryotic chromosomes. When the cell is not dividing, this exists in its dispersed form, as a mass of very long, thin fibers that are not visible with a light microscope
Nucleolus
A specialized structure in the nucleus, consisting of chromosomal regions containing ribosomal RNA (rRNA) genes along with ribosomal proteins imported from the cytoplasm; site of rRNA synthesis and ribosomal subunit assembly
Ribosome
A complex of rRNA and protein molecules that functions as a site of protein synthesis in the cytoplasm; consists of a large and a small subunit. In eukaryotic cells, each subunit is assembled in the nucleolus
Endomembrane System
The collection of membranes inside and surrounding a eukaryotic cell, related either through direct physical contact or by the transfer of membranous vesicles; including the plasma membrane, the nuclear envelope, the smooth and rough endoplasmic reticulum, the Golgi apparatus, lysosomes, vesicles, and vacuoles
Vesicle
A membranous sac in the cytoplasm of a eukaryotic cell
Endoplasmic Reticulum (ER)
An extensive membranous network in eukaryotic cells, continuous with the outer nuclear membrane and composed of ribosome-studded (rough) and ribosome-free (smooth) regions
Smooth ER
The portion of the endoplasmic reticulum that is free of ribosomes
Rough ER
That portion of the endoplasmic reticulum with ribosomes attached
Glycoproteins
A protein with one or more covalently attached carbohydrates
Transport Vesicles
A small membranous sac in a eukaryotic cell’s cytoplasm carrying molecules produced by the cell
Golgi Apparatus
An organelle in eukaryotic cells consisting of stacks of flat membranous sacs that modify, store, and route products of the endoplasmic reticulum and synthesize some products, notably noncellulose carbohydrates
Lysosome
A membrane-enclosed sac of hydrolytic enzymes found in the cytoplasm of animal cells and some protists