Cell signalling Flashcards
- Oncogenes and tumour suppressors talk to each other - Complexity and mechanisms of cell signalling - The main components of cell signalling - Types of ligands and receptors - Major post-translational modifications and the contribution of Dundee scientists - How RTKs play a role in cancer - Targeting kinases in cancer: Malignant melanoma - Ubiquitin proteasome system
1
Q
Types of signal molecules (8)
A
- growth factors
- hormones
- interleukins
- interferons
- chemokines
- extracellular matrix proteins
- toxins
- neurotransmitters
2
Q
What is angiogenesis
A
the formation of new blood cells
3
Q
Different modes of signal transmission (4)
A
- conformational-coupling (performed complex)
- conformational-coupling (diffusion-dependent complex formation)
- posttranslational modification
- protein degradation
4
Q
Discovery of Src
A
- Rous discovered Sarcoma in chickens was transmissible and a virus
- V-Src can transfer Src to avian species and cause cancer (chickens only)
- V-Src is identical to protooncogene C-Src (no infection needed)
5
Q
Targeting RTKs for therapy
A
- EGFR is one of the most highly mutated oncogenes
- targeting RTKs involves inhibiting or blocking their activity using drugs or degradation / downregulation
6
Q
EGFR mutation
A
- one of the most highly mutated oncogenes (pro-growth)
- gene encodes for RTK
- constantly sends cells through the cell cycle
7
Q
How can EGFR gene be mutated
A
- amplification (overexpression of receptors)
- point-mutation (regulated by ligand binding)
- deletion (unregulated)
8
Q
HER2+ cancers
A
- e.g. breast cancers
- mutation (prob amplification) drives cell through cell cycle
- cells dimerise, forming heterodimers, leading to phosphorylation of tyrosine on cytoplasmic tails of EGFR, and go through cell cycle
9
Q
HER2- cancers
A
- gene is not mutated or diving proliferation of cells
10
Q
How can cancer develop with no mutation in EGFR gene
A
- changes in ligands of receptors
- overexpression
11
Q
Targets for cancer treatments
A
- TKI (tyrosine kinase inhibitors)
- monoclonal antibodies
12
Q
Action of TKIs
A
- bind ATP binding pocket, mimicking ATP
- either competitive or non competitive
- kinase cannot phosphorylate substrate and signal will not be sent for cell to go through cell cycle
- cell arrest
13
Q
The action of monoclonal antibodies
A
- antibodies isolated in lab
- bind the extracellular domain (ectodomain) of some/all of EGFR family members
- block ligand binding
- block dimerisation
- recruit immune cells
- deliver toxins to tumour
14
Q
Why is it essential to treat all 7 types of EGFR family member mutations
A
- ## EGFR acts differently depending on which region of the body it is in
15
Q
Malignant melanoma
A
- can be benign or malignant
- more than 70,000 cases a year in USA with 8800 deaths
- White people are 10x more vulnerable than African Americans
- one of the most common cancers in people under 30
