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Module 1 CB > Cell Org II > Flashcards

Cell Org II Flashcards

1
Q

5 components of cell nucleus

A
  1. Nuclear envelope (NE)
  2. Nuclear lamina (NL)
  3. Nuclear Pore Complexes (NPCs)
  4. Nucleolus
  5. Nucleoplasm
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2
Q

Describe the NE

A
  1. Inner nuclear membrane
    1. Faces the nucleoplasm
    2. In contact with the nuclear lamina
      1. functions as a scaffold stabilizing the nuclear envelope
  2. Outher nuclear membrane
    1. Faces the cytoplasm
    2. Continuous with ER
    3. Cytoplasmic surface contains ribosomes (rER)
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3
Q

Describe the nuclear lamina

A
  1. Nuclear lamina
    1. Thin, sheet like meshwork beneath the inner nuclear membrane
    2. Scaffold for nuclear envelope, chromatin and nuclear pores
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4
Q

Describe the nuclear pore complex (NPC)

A
  1. Spans the inner and outer nuclear membranes (80-100nm in diameter)
  2. 2000-3000 NPCs in a typical nuclear envelope
  3. Allows transport of molecules between the nucleus and cytoplasm
    1. 9nm is the threshold between free/active transport
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5
Q

describe the Nucleolus (the 3 zones)

A
  1. Site of ribosome production
    1. Fibrillary center
      1. DNA loops of 5 chromosomes
      2. Contains rRNA genes
    2. Fibrillar material
      1. Transcription of rRNA genes
    3. Granular material
      1. Initial ribosomal assembly
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6
Q

describe chromatin (2 types)

A
  1. Chromosomes in various degrees of uncoiling
  2. Packaged into nucleosomes
    1. Complex of DNA and proteins called histones
  3. 2 Types
    1. Euchromatin (EC)
      1. Lightly staining
      2. Less condensed chromatin, which makes it more transcriptionally active
    2. Heterochromatin (HC)
      1. Densely staining
      2. Highly condensed (HC) chromatin, which makes it less transcriptionally active
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7
Q

describe the nucleosome

A
  1. It is the fundamental structural unit of chromatin
  2. Macromolecular complex, like beads on a string
  3. Found in both EC and HC
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8
Q

describe where ribosomes are made and what they are

A
  1. They are made in the nucleolus and leave the nucleus and enter the cytoplasm
  2. Consists of 2 ribosomal RNA subunits and associated proteins
  3. Sites of protein synthesis (translation)
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9
Q

describe the 2 types of ribosomes

A
  1. Membrane-bound
    1. Attached to cytoplasmic surface of the ER
    2. Synthesize proteins that are being translocated into the ER lumen
    3. Produce
      1. Lysosomal proteins
      2. Secereted proteins
      3. PM proteins
  2. Free ribosomes
    1. Unattached to any membrane
    2. synthesize all other proteins
      1. nuclear proteins
      2. mitochondrial proteins
      3. cytosolic proteins
      4. peroxisomal proteins
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10
Q

describe the structure of the ER

A
  1. Interconnected network of branching tubules and flattened sacs distributed throughout the cytoplasm
  2. At certain sites, the ER membrane is continuous with the other NE membrane
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11
Q

describe the smooth ER

A
  1. Smooth ER: functions in the synthesis of lipids and detox
    1. Region of the ER that lacks bound ribosomes
    2. Abundant in cells specialized in lipid metabolism
      1. This makes it well developed in cells that synthesize and secrete steroids
    3. Plays a major role in detox
    4. Also functions to sequester calcium in muscle cells/sarcoplasmic reticulum
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12
Q

describe the rough ER

A
  1. Rough ER functions in the synthesis of proteins destined for PM, lysosomes or secretion
  2. Only mRNA molecules with an ER signal sequence bind to rER membranes
  3. Abundant in cells specialized in protein synthesis
    1. Secretory cells producing proteins for extracellular export
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13
Q

describe structure and location of GA

A
  1. Complex of flattened, membrane-enclosed cisternae
  2. Usually located next to the nucleus and centrosome
    1. Location is microtubule dependent
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14
Q

describe the 2 faces of the GA

A
  1. Cis face
    1. Entry
    2. Cis Golgi Network (CGN)
  2. Trans face
    1. Exit
    2. Trans Golgi Network (TGN)
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15
Q

which cells would the GA be most developed?

A
  • Secretory cells
    • Plasma cells, which secrete antibodies
    • Pancreatic acinar cells, which secrete digestive enzymes
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16
Q

describe the differences between COP I and COP II

A
  • COP-I coated vesicles
    • Retrograde transport
    • CGN back to rER
  • COP-II coated vesicles
    • Anterograde transport
    • Carry newly synthesized proteins from rER to CGN
17
Q

describe the 3 functions of the GA

A
  1. Post-translational modification
    1. Glycolysation
    2. Sulfation
    3. Phosphorylation
    4. Proteolysis
  2. Sorting
  3. Packaging
18
Q

describe the different types of vesicular trafficking in the GA

A
  1. Constitutive
    1. Constantly prouducing vesicles for secretion
      1. Fibroblasts secreting ECM components
  2. Regulated secretory pathway
    1. Vesicles are stored and secreted with stimulus
      1. Neurons, beta cells (endocrine) and acinar cells (exocrine) of pancreas
  3. Lysosomal pathway
    1. Precursor lysosomal hydrolases are covalently modified by the addition of M6P in the GA
    2. the TGN recognizes this for transport to lysosomes
19
Q

describe the structure and function of lysosomes

A
  1. Digestive organelles
    1. Function in the controlled intracellular digestion of macromolecules
  2. Membrane-enclosed compartments filled with ~40 types of hydrolytic enzymes
    1. Require an acidic environment, around pH 4.7
20
Q

How many lysosomal storage diseases are there and what are the results?

A
  • 1:7000 live births
  • Results
    • Accumulation of undigested products
    • Disruption of normal cell function
    • Cell death
21
Q

describe Tay-Sachs (gene location, results)

A
  1. First LSD
  2. Deficiency of HEXA
  3. Gene location: chromosome 15q24.1
  4. Results in the accumulation of GM2 ganglioside
  5. Death of neurson in the brain and spinal cord
22
Q

what are the pathways that lead to digestion by lysosomes?

A
  1. Phagocytosis
  2. Endocytosis
  3. Autohphagy (self-eating): major cellular pathway to degrade proteins and organelles
    1. intracellular membrane surrounds the organelle and cytoplasm
    2. autophagosome formed (double membraned vacuole)
    3. fusion of autophagosome with lysosome
    4. contents are degraded, recycled and reused
23
Q

describe proteasomes and how they function

A
  • Proteasomes are large protein complexes involved in protein degradation
  • ATP-dependent
  • Destroys proteins without involving lysosomes
24
Q

how do proteasomes destroy proteins and which do they target?

A
  • Polyubiquitination
    • Proteins targeted for destruction are covalently tagged with the ubiquitin protein
  • Destruction of abnormal proteins and normal short-lived regulatory proteins
    • Ex: cyclins, transcription factors, tumor suppresors
  • Proteasome inhibitors used as anti-cancer agents
    • proteasome inhibition may prevent degradation of pro-apoptotic factors such as the p53 protein
25
Q

what are inclusions and name the 3 pigments

A
  • Inclusions are cytoplasmic or nuclear structures formed from metabolic products of the cell
  • Pigments: membrane bound
    • Lipofuscin
        1. Brownish-gold pigment
      • Generally seen in non-dividing cells
      • Conglomerate of lipids, metals, organic molecules
    • Hemosiderrin
        1. Brown pigment
        1. Iron-storage complex found in the cytoplasm
      • Likely formed by indigestible residues of hemoglobin
        • Easily seen in spleen
    • Melanin
      • Brown pigment
26
Q

name 2 storage forms of inclusions

A
  1. Glycogen
    1. Non-membrane bound, TEM dense bodies
    2. Storage form of glucose
      1. catabolism releases glucose for energy
  2. Lipid
    1. Non-membrane bound, TEM dense
    2. Fat droplets
    3. Energy store and source of short carbon chains for membrane synthesis
    4. Lipid stroage disease (lipidoses)
      1. Lipid droplets accumulate in abnormal amounts or locations
27
Q

describe the 2 membranes of the mitochondria

A
  1. Outer
    1. Contains porins
      1. Large channel-forming proteins, which allow free passage of small molecules <5000 daltons
  2. Inner
    1. Thinner and thrown into folds
      1. Cristae
    2. 3 major functions
      1. Oxidation reactions
        1. Respiratory ETC
      2. Synthesize ATP
        1. ATP synthase
      3. Regulate metabolite transport into and out of the matrix
28
Q

describe the 2 compartments of the mitochondria

A
  1. Intermembrane space
    1. Space between the inner and outer membranes
    2. Oxidative phosphorylation takes place here
    3. Contains specific enzymes that use ATP generated by the inner membrane
      1. Creatine kinase
      2. Adenylate kinase
      3. Cytochrome C
        1. Important factor in intrinsic apoptosis pathway
  2. Matrix
    1. Mixture of hundreds of enzymes
      1. Oxidation of pyruvate and fatty acids
      2. Krebs Cycle
    2. Matrix granules
      1. Storage site for Ca2+ and other divalent cations
    3. Mitochondrial DNA, ribosomes and tRNAs
29
Q

what is the primary function of the mitochondria

A
  1. generate ATP
    1. Krebs Cycle
    2. OP
    3. Beta oxidation of fatty acids
  2. Abundant in cells that generate and expend large amount of energy
30
Q

describe mitochondrial diseases and the 2 types described

A
  • Results in defects in MT enzymes required for ATP production
    • Dysfunctional mitochondrial respiratory chain
  • Affects tissues that require large amounts of ATP
    • Neurons and muscle cells
  • Myoclonic elilepsy with ragged red fibers (MERRF)
    • Mutation in the tRNA gene
    • MT-TK
  • Leber hereditary optic neuropathy
    • Mutations in the MT-ND1, MT-ND4, MT-ND4L or MT-ND6
31
Q

what role do peroxisomes have and what synthesizes them

A
  • Important role in fat metabolism
    • Very long chain fatty acid (VLCFA) beta-oxidation
    • Catalyze initial reactions in formation of plasmalogens
      • Abundant class of phospholipids in myelin
      • Deficiencies cause profound abnormalities in myelination of nerve cells
  • Peroxisomal proteins are synthesized by free (cytoplasmic) ribosomes
    • Proteins must have a peroxisomal targeting signal
32
Q

how do peroxidases function and which cell are they particularly important in

A
  • Specialized to compartmentalize and degrade toxic reactive oxygen moleculesContains catalase and other peroxidases
    • Converts H2O2 to O2 and H2O
  • Particularly in hepatocytes
    • Detox of ingested alcohol
33
Q

describe peroxisomal diseases

A
  • Dysfunctional peroxisomes
  • Zellweger Syndrome
    • Mutations in genes required for peroxisome function
    • Defective import of peroxisomal proteins

Module 1 CB (18 decks)

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