Cell integrity

Question 1

Overall equations for ETC

Answer 1

NADH + H+ + 0.5 O2 —> NAD+ + H2O

Question 2

Name complex 1 and it’s details

Answer 2

NADH dehydrogenase, higher affinity for e- than NADH so reduces it to NAD+, 2e-, H+

Question 3

name complex 2 and it’s importance

Answer 3

Succinate dehydrogenase, doesn’t pump protons, FADH2 goes directly to this and as complex 1 is bypassed less protons are transported by FADH2

Question 4

name complex 3

Answer 4

Q-cytochrome oxireductase

Question 5

name complex 4

Answer 5

cytochrome C oxidase

Question 6

name the 2 transport proteins

Answer 6

Co-enzyme Q or Ubiquinone and cytochrome C

Question 7

Discuss ATP synthase

Answer 7

has membrane bound part F0 and F1 which projects into matrix, chemiosmosis makes the rotor rotate and so conformational energy flows from subunit to ADP and Pi to promote ATP formation

Question 8

Metabolic poisons- cyanide and azide

Answer 8

bind with high affinity to Fe3+ in cytochrome C oxidase complex blocking ETC

Question 9

Metabolic poisons- Malonate

Answer 9

competitive inhibitor of succinate dehydrogenase, slows down flow of electrons from succinate to ubiquinone

Question 10

Metabolic poisons- Rotenone

Answer 10

inhibits e- from complex 1 to coenzyme Q

Question 11

Metabolic poisons- Oligomycin

Answer 11

bonds to stalk of ATP synthase and blocks flow of protons

Question 12

Metabolic poisons- dinitrophenol

Answer 12

shuttles protons across inner mitochondrial membranes, uncoupling OP from ATP production