(Cell) Glycolysis Flashcards
What are the two phases of glycolysis?
Energy investment phase
Energy payoff phase
Step 1
An enzyme (hexokinase) catalyses the phosphorylation of glucose with ATP, forming glucose-6-phosphate and producing ADP
Step 2
An isomerase (phosphoglucoisomerase) converts the glucose-6-phosphate into an isomer, fructose-6-phosphate
Step 3
An enzyme (phosphofructokinase) catalyses the phosphorylation of fructose-6-phosphate, producing fructose-1,6-biphosphate and ADP
Step 4
An enzyme (aldolase), cleaves fructose-1,6-biphosphate into dihydroxyacetone phosphate and glyceraldehyde-3-phosphate, both are 3 carbon sugars
Step 5
An isomerase (Triose phosphate isomerase) transforms dihydroxyacetone phosphate into glyceraldhyde-3-phosphate, thus the result of the first half of glycolysis is two glyceraldehyde-3-phosphate
The first _ steps are part of the energy investment phase
5
Step 6
An enzyme (glyceraldehyde-3-phosphate dehydrogenase) oxidizes the sugar, forming 1,3-biphosphoglycerate, extracting electrons carried away by NAD+ (producing NADH), phosphate group added in
Step 7
An enzyme (phosphoglycerokinase) catalyses the substrate level phosphorylation of 1,3-biphosphoglycerate with ADP, forming 3-phosphoglycerate and ATP
Step 8
An enzyme (phosphoglyceromutase) catalyses the reaction, forming 2-phosphoglycerate
Step 9
An enzyme (enolase) catalyses the reaction of taking out water to form phosphoenolpyruvate
Step 10
An enzyme (pyruvate kinase) catalyses the substrate level phosphorylation of phosphoenolpyruvate into pyruvate and producing ATP