cell fractionation

Question 1

what is cell fractionation

Answer 1

process where cells are broken up and the different organelles are separated out

Question 2

in what condition should the organelles be in and why ?

Answer 2

.cold- reduce enzyme activities that might break up organelles
.isotonic-to prevent osmosis so no organelles burst or shrink
.buffered-too maintain pH levels

Question 3

what are the steps of cell fractionation?

Answer 3

1.homogenisation
2.filtration
3.ultracentrifugation

Question 4

describe the process of homogenisation

Answer 4

cells are broken up by a homogeniser( blender) releasing organelles

Question 5

describe process of filtration

Answer 5

. using a sieve or muslin, large pieces of debris or complete cells are removed

Question 6

describe centrifuging

Answer 6

1.tube filtrate is placed in centrifuge and spun at slow speed
2. nuclei is forced at the bottom at it is the most dense
3. supernatant is removed and transferred to another tube and RESPIN AT A HIGHER SPEED
4. process is repeated in order of density nuclei, chloroplasts, mitochondria, lysosomes etc.