Cell Division Flashcards

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1
Q

What are the two phases of cell division?

A

Nuclear Division and Cytokinesis

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2
Q

What are the types of nuclear division?

A

Mitosis & Meiosis

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3
Q

What is the first step in both mitosis and meiosis?

A

Condensation of genetic material, chromatin, into chromosomes

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4
Q

What are sister chromatids?

A

identical halves that make up a chromosome - the sister chromatids are joined at the centromere

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5
Q

Describe interphase of the cell cycle

A

Cell division does not occur here - includes G0, G1, S, G2.

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6
Q

When does replication and growth take place in the cell cycle?

A

S phase of interphase

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7
Q

Describe the M Phase of the cell cycle

A

This is where cell division occurs - Mitosis (Prophase, Metaphase, Anaphase, Telophase) and Cytokinesis

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8
Q

What happens in prophase?

A

Condensation - Nucleoli disappear and chromatin condenses into chromosomes. The nuclear envelope breaks down and mitotic spindle is formed. Microtubules connect to kinetochore and MTOCs move towards opposite poles.

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9
Q

What happens in metaphase?

A

Alignment - chromosomes align across the metaphase plate and microtubules

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10
Q

What happens in anaphase?

A

Separation - chromosomes separate into chromatids (which is referred to as a chromosomes now) - microtubules condense towards opposite poles until each pole has a complete set of chromosomes.

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11
Q

What happens in telophase?

A

Restoration - Nuclear envelope is restored around each pole, forming two nuclei. Chromosomes within nuclei disperse into chromatin and nucleoli reappear —-Opposite of what happens in prophase.

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12
Q

What is cytokinesis?

A

The division of the cytoplasm to form two cells.

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13
Q

What structure is formed in plants following cytokinesis?

A

Cell plate. Vesicles from Golgi migrate to plane between two newly forming nuclei, fusing for form a cell plate which will become the plasma membranes for the two cells. Cell walls develop between membranes.

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14
Q

What structure is formed in animals following cytokinesis?

A

Cleavage furrow. Microfilaments form a ring inside the plasma membrane between the two newly forming nuclei and pull plasma membrane to center, dividing the cell into two daughter cells.

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15
Q

What is the difference between actin filaments and microfilaments?

A

Nada. Same shit. Microfilaments are made up of polymers of actin.

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16
Q

Where and when does crossing over take place?

A

During synapsis at the chiasmata (close associations between non-sister chromatids of homologues) of Prophase I. This DOES NOT happen in Prophase II

17
Q

How does metaphase I differ from metaphase II?

A

(I). Homologous pairs of chromosomes are spread across plate - INDEPENDENT ASSORTMENT HAPPENS HERE

(II) Singular chromosomes are spread across the plate

18
Q

How does Anaphase I differ from Anaphase II?

A

(I). Homologues uncouple (II). Chromosomes separate into two chromatids

19
Q

How does Telophase I differ from Telophase II?

A

(I). Each pole contains half the number of chromosomes (uncoupled chromatids) - 2 haploid cells are formed. (II). Each pole contains half the number of chromosomes (chromatids) - 4 haploid cells are formed.

20
Q

What types of cells undergo mitosis?

What types of cells are produced?

A

Somatic cells, which are all body cells except those that produce eggs and sperm (or pollen)

21
Q

What types of cells undergo meiosis?

What types of cells are produced?

A

Reproductive cells (ovaries, testes, anthers) - gametes: eggs, sperm, pollen are produced.

22
Q

What is syngamy?

A

Fertilization - fusion of egg and sperm to give rise to a zygote

23
Q

What happens after zygote is formed?

A

The zygote undergoes mitosis to produce a multicellular organism.

24
Q

What are sources of genetic variation?

A

Crossing over (prophase I), Independent assortment (metaphase I), and joining of gametes

25
Q

What happens when (S/V) is high?

A

This means that the cell can function adequately because it has enough surface area for proper function and interaction with outside environment

26
Q

What happens when (S/V) is low?

A

This means the cell’s volume has exceeded it’s surface either cell growth stops of cell division begins.

27
Q

What happens to (G/V) as cell grows?

A

G/V decreases and because the cell has a finite amount of genetic material, the cell cannot be adequately supplied with enzymes and other biosynthetic substances.

28
Q

What are examples of cells that are genetically programmed not to divide?

A

Muscle and nerve cells - they remain in G0 rarely dividing after they have matured.

29
Q

What are the three checkpoints of the cell cycle and where do they occur?

A

G1 checks for DNA quality (end of G1). G2 checks for accuracy of DNA replication (at the end of G2). M (during metaphase) ensures microtubules are properly attached to all kinetochores at metaphase plate before ANAPHASE

30
Q

What are Cdks?

A

Cyclin-dependent kinases - enzymes responsible for advancing the cell past checkpoints and through the cell cycle. They activate other proteins by phosphorylating them.

31
Q

How are Cdks activated?

A

By the attachment of cyclins - and then complete activation by phosphorylation

32
Q

Describe the function of Mitosis-Promoting Factor (or maturation-promoting factor) (MPF)

A

MPF is a cyclin-Cdks complex that advances the cell cycle through G2 checkpoint.

33
Q

What are external factors that influence the cell cycle?

A

Growth factors (e.g. PDGF), density-dependent inhibition, anchorage dependence

34
Q

Why is cancer referred to as a disease of the cell cycle?

A

Transformed, cancerous cells, proliferate without regard to the cell cycle checkpoints, and other regulatory mechanisms.