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Cancer - cell cycle > Cell Cycle 3 > Flashcards

Cell Cycle 3 Flashcards

1
Q

What are the Oncoproteins from Adenovirus?

A

E1A, E1B

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2
Q

What is the weight of retinoblastoma?

A

105kDa

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3
Q

What proteins are similar to Rb?

A

p107 and p130

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4
Q

When hypo-phosphorylated, what transcription factor is Rb bound to to prevent its function?

A

E2F

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5
Q

Whats releases E2F?

A

CyclinD/cdk4 or 6

or E7, E1A

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6
Q

What are transcriptional targets of E2F?

A

Cdk1, PolA, DHFR, E2F1, Cyclin A

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7
Q

What is DP?

A

The dimerisation partner of E2F

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8
Q

What sites are there in E2F?

A

Cyclin binding, DNA binding, Dimerisation, Transactivation, Rb binding

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9
Q

What is the E2F target sequence?

A

TTTCGCGC

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10
Q

What phases is E2F active in?

A

G1 and S phases - cyclin A promotes destruction of E2F, hence the cyclin binding site

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11
Q

What is the ‘go’ signal for the restriction point?

A

phosphorylation of Rb and release of E2F

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12
Q

What are the domains in RB?

A

N terminal domain, A domain, Spacer, B domain, C terminal domain

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13
Q

What motif do oncoproteins use to bind to domain B of Rb?

A

LxCxE

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14
Q

Can E2F bind DNA when bound to Rb?

A

Yes

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15
Q

Does Rb interact with:

a) HDAC
b) HAT

A

HDAC

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16
Q

Does E2F interact with:

a) HDAC
b) HAT

A

HAT

17
Q

What is BRG1 and what is the relevance to the restriction point?

A

ATP dependant chromatin remodeller. Can slide nucleosomes along DNA and can loosen or tighten DNA

18
Q

What does cyclinD/cdk4 phosphorylate on Rb?

A

Residues in CTD

19
Q

What happens directly after phosphorylation of CTD of Rb?

A

Interaction between CTD phosphates and lysines in binding pocket (ASB)
This cases dissociation of HDAC from B domain and exposes ser-567

20
Q

What does loss of HDAC from Rb allow?

A

Expression of cyclin E - under promoter sensitive to chromatin structure - HAT is still bound to loosen chromatin

21
Q

How does cyclin E/cdk2 contribute towards the release of E2F?

A

Phosphorylates Ser-567, only available when Rb is in kinked conformation - this frees E2F

22
Q

When does BRG1 dissociate from Rb in the phosphorylation pathway?

A

It does not need to

23
Q

What oncoproteins have structural similarities to E7?

A

E1A, large T antigen

Cancer - cell cycle (9 decks)

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