cell biology and microscopes Flashcards
how does a light microscope work?
uses light to illuminate objects under view, convex lens manipulates how light enters the eye
how does an electron microscope work?
lens replaced by electromagnets that ‘bend’ the electron beam. forms an image as a photo - elctron micrograph
radiation source of electron microscope
Electrons
radiation source of light microscope
visible light
max magnification of electron microscope
approx ×1 000 000
max magnification of light microscope
approx ×2 000
resolution of electron microscope
approx 0.1nm
resolution of light microscope
approx 200nm
images seen through electron microscope- colour
black and white
images seen through light microscope- colour and why
coloured - colour is a property of light
types of electron microscope
TEM and SEM
TEM microscope meaning
transmission electron microscope
how are images seen through a tem and why?
2D - electron beams pass through thin slices of specimen, analyses inner structures
how are images seen through a sem and why?
3D - Specimen coated in gold so electrons bounce off and give outside view
what are prokayotes?
single celled organisms, don’t have nuclei or organelles, are much smaller than eukaryotic, and always have cell walls
egs of prokaryotes
bacteria and archaea
what are eukaryotes?
mostly multi celled organisms like animals and plants, have a nucleus to contain and organelles
what happens in anaphase?
away- move to poles of cell
formula linking actual size,magnification and image size
i = a × m
function of cell membrane
controls the movement of substances in and out
cell membrane is found in ….
animal and plant cells
cell sap function
store of minerals and water found in vacuole
cell sap is found in ….
plant cells
cellulose cell wall function
supports the cell and keeps its shape
cell wall is found in ….
Plant cells
Chloroplast function
respiration occurs here, releasing energy
chloroplast is found in ….
Plant cells
function of mitochondria
respiration occurs here, releasing energy
mitochondria are found in…
Plant and animal cells
cytoplasm function
where chemical reactions of the cell take place
cytoplasm function
-where chemical reactions of the cell take place
nucleus function
contains dna and controls the activity of the cell
nuclei are found in…
animal and plant cells
ribosomes function
proteins are made/synthesised here
ribosomes are found in….
animal and plant cells
vacuole function
full of cell sap, maintains shape of cell
permanent vacuoles are found in …
Plant cells
features of prokaryotic cell
-no membrane bound nucleus
-dna is in a nucleoid floating in cytoplasm
-ribosomes
-cell wall
-much smaller than eukaryote
main organelles in a plant cell (8)
- vacuole
- cell wall
- cell membrane
- nucleus
- ribosomes
- chloroplasts
- mitochondria
- cytoplasm
main organelles in an animal cell (5)
- cell mebrane
- cytoplasm
- mitochondria
- nucleus
- ribosomes