Cell Biology Flashcards
What do you use to see the ultrastructure of a cell?
Electron microscope
Chloroplasts are the site of?
Photosynthesis
Mitochondria are the site of?
Respiration
What is the function of the cell membrane?
Controls what goes in and out of the cell
What is the function of the cell wall?
Keeps cell shape and supports the cell
What is the function of the chloroplasts?
To photosynthesize
What is the function of the vacuole?
To store sugar and salts
What is the function of the nucleus?
Containing genetic information and controls cell activity
What is the function of the cytoplasm?
Site of biochemical reactions
What is the function of chromosomes?
Controls transfer of genetic material
What is the function of ribosomes?
Protein synthesis
What is the function of plasmids?
Transfer a gene from one organism to another
What is the function of mitochondria?
Respiration
What are plant cell walls made out of?
Cellulose
What are cell membranes made out of?
Proteins and phosolipids
What is the structure of the cell membrane?
Phosolipid bi-layer
How do you describe the structure of a cell membrane?
Fluid mosaic
What are the two types of transport?
Passive and active
What is the definition of diffusion?
Diffusion is the passive movement of molecules of a substance from an area of high concentration to an area of low concentration down a concentration gradient
What is the definition of osmosis?
Osmosis is the movement of water molecules from a higher water concentration to a lower water concentration through a selectively permeable membrane, down a concentration gradient
What happens if the water concentration is greater outside the cell than in?
Water will pass into the cell and the cell will become turgid or lysed
What happens if the water concentration is lower outside the cell than in?
Water will pass out of the cell and the cell will become flaccid and then plasmolysed
What is active transport?
The movement of molecules against their concentration gradient and requires ATP
What is mitosis?
Mitosis is a type of cell division, which produces daughter cells that are genetically identical to the parent
How many chromosomes does a human have?
46
What is the number of chromosomes in the nucleus called?
Its chromosomes complement
What is the first step in mitosis?
The chromosomes unravel
What is the second step in mitosis?
The chromosomes duplicate and form a chromatid with a centromere joining the two chromosomes
What is the third step in mitosis?
The nucleus membrane breaks down and the chromatids line the equator of the cell. Spindle fibres then attach to the individual chromatids
What is the fourth step in mitosis?
The spindle fibres pull, breaking the chromatid into chromosomes at either pole of the cell
What is the fifth step in mitosis?
A nuclear membrane forms round the chromosomes and the original cell membrane pinches and starts to separate
What precautions should you take when using an aseptic technique?
Sterilise all equipment before and after Cover up any cuts Sterilise work surface before and after
What are chromosomes made out of?
Two strands of DNA (deoxyribonucleic acid) twisted in double helix shape
What are the four bases?
A=Adenine T=Thymine C=Cytosine G=Guanine
Which bases pair with which?
A with T and C with G
What holds the two strands of DNA together?
Hydrogen bonding
What are proteins made out of?
Amino Acids
What carries the genetic code to the ribosome for protein synthesis?
Ribonucleic acid (mRNA)
How does the ribosome produce proteins?
Each three bases code for one amino acid (triplet code). The amino acids then join together by peptide bonds to form proteins
What are the two different structures of proteins?
Globular and fibrous
When would proteins have a globular shape?
When they are hormones, enzymes and antibodies
When would proteins have a fibrous shape?
When they are keratin, collagen or muscle fibres (structural role)
What does an antibody defend against?
Pathogens
What are enzymes?
Biological catalysts
How is an enzyme specific to one substrate?
They are folded in a way so their active site is specific to on substrate. This is called the lock and key theory
Examples of when enzymes are used to degrade?
Proteins to amino acids Carbohydrates to sugars Fats to fatty acids and glycerol
Examples of when enzymes are used to synthesize
Building up proteins, carbohydrates and lipids
What is produced when hydrogen peroxide is broken down by catalase?
Water and oxygen
What is the first step in genetic engineering?
The required gene is cut out by enzymes
What is the second step in genetic engineering?
A bacterial plasmid is removed and cut open using enzymes
What is the third step in genetic engineering?
The required gene is inserted into the plasmid and sealed
What is the fourth step in genetic engineering?
The plasmid is inserted into another suitable bacterium
What is the fifth step in genetic engineering?
The bacteria are left to reproduce
What is the sixth step in genetic engineering?
The modified bacteria produce the product and its purified
What is the first stage in photosynthesis?
Photolysis
What is the second stage in photosynthesis?
Carbon Fixation
How is light used in photosynthesis?
Light energy is trapped in the chloroplasts and is used to create ATP from ADP+Pi. It is also used to split water into oxygen into hydrogen
What happens to the oxygen in photosynthesis?
It diffuses out of the cell through the stoma or used in respiration
What happens in carbon fixation?
Carbon dioxide undergoes a series of enzyme controlled reactions and with the energy released by the ATP, joins together with hydrogen to form glucose
What are the main limiting factors of photosynthesis?
Carbon Dioxide, light intensity and temperature
Where does respiration take place?
In the cytoplasm and in the mitochondria
What is the first stage of Respiration?
Glycolysis
What does the first stage of respiration involve?
Glucose gets broken down into pyruvate in the cytoplasm and 2 ATP are produced
What is the second stage of respiration?
The breakdown of pyruvate
Label organells X,Y and Z
X=Chloroplasts
Y=Ribosome
X=Mitochondria
What is produced in the first stage of respiration?
Two molecules of pyruvate and ATP
What happens in the second stage of aerobic respiration?
Pyruvate reacts with oxygen and breaks down forming 36 molecules of ATP, carbon dioxide and water
How many molcules of ATP are produced in aerobic respiration?
38
What happens in the second stage of respiration if oxygen isn’t present?
The pyruvate will break down into lactic acid
What happens when the second stage of respiration in plants doesn’t recieve oxygen?
The pyruvate breaks down to form ethanol and carbon dioxide