Cell Biology Flashcards
Eukaryotic cells
- complex
- include all animal and plant cells
Prokaryotic cells
- small and simpler
- bacteria
What subcellular structures does an animal have
Nucleus
Cytoplasm
Cell membrane
Mitochondria
Ribosomes
Extra things that a plant cell has that animal doesn’t
Cell wall
Permanent vacuole
Chloroplasts
What is in a bacterial cell
Cytoplasm
Cell membrane
Cell wall
Singular strand of DNA
Might have small rings of DNA called plasmids
Formula for magnification
Magnification= image size/real size
Light microscopes
-use light and lenses
-let us see individual cells and large subcellular structures
Electron microscopes
-use electrons to form an image
-have higher magnification and higher resolution
-we can see smaller things in more detail
How to prepare a slide to view onion cells (experiment)
- Add a drop of water to the middle of a clean slide
- Cut up an onion and separate it out into layers. Use tweezers to peel off some epidermal tissue from the bottom of one of the layers
- Using the tweezers, place the epidermal tissue into the water on the slide
- Add a drop of iodine solution. It is a stain.
- Place a coverslip on top. Try not to get air bubbles under as they obtrude the view of the specimen
What is a stain
-used to highlight objects in a cell by adding colour to them
How to use light microscope (experiment)
- Clip the slide onto stage
- Select lowest powered objective lens
- Use the coarse adjustment knob to move the stage up to just below the objective lens
- Look down eyepiece. Use coarse adjustment knob to move the stage downwards until the image is roughly in focus
- Adjust focus with fine adjustment knob until you get a clear image of what’s on the slide
- If you need to see the slide with greater magnification, swap to a higher powered objective lens and refocus
Name all parts if light microscope
- Eyepiece
- High and low power objective lenses (the long and short bits)
- Coarse adjustment knob (big wheel on side)
- Fine adjustment knob (smaller wheel on side below stage)
- Stage (where slide goes)
- Light (below stage)
What is differentiation
The process by which a cell changes to become specialised for its job
Sperm cell differentiation
-differentiate for reproduction
-long tail and streamlined head to help it swim to the egg
-lots of mitochondria in the cell to provide the energy needed
-carries enzymes in its head to digest through the egg cell membrane
Nerve cells differentiation
-specialised for rapid signalling
-carry electrical signals from one part if the body to another
-they’re long (to cover more distance)
-have branched connection at their ends to connect to other nerve cells and from a network throughout the body
Nucleus
Contains genetic material that controls the activities of the cell
Cytoplasm
Gel like substance where most of the chemical reactions happen. Contains enzymes that control these chemical reactions
Cell membrane
Holds the cell together and controls what goes in and out
Mitochondria
These are where most of the reactions for aerobic respiration take place
Ribosomes
Where proteins are made in the cell
Cell wall
Made of cellulose, supports cell and strengthens it
Permanent vacuole
Contains cell sap, weak solution of sugar and salts
Chloroplasts
Where photosynthesis occurs. Contains chlorophyll
Muscle cells differentiation
-Specialised for contractions
-it’s long so they have space to contract
-contain lots of mitochondria to generate energy needed for contraction