Cell biology Flashcards
Outline cell theory + atypical examples
3 elements:
- living things are composed of cells
- cells are the smallest unit of life
- all cells come from existing cells
exceptions:
- muscle fibers are very long (multiple nuclei)
- most fungi have hyphae (thread structure with many nuclei)
- giant algae are much larger
List the 7 functions of life (Mr H. Nerg)
- metabolism: chemical reactions
- reproduction: making offspring
- homeostasis: keeping inside conditions controlled
- nutrition: getting food
- excretion: removal of waste products
- response: responding to stimuli
- growth: increase in size
what are the 7 functions of life in paramecium
- meta: yeast turns blue in cytoplasm
- rep: cell division thru mitosis
- homeo: contractile vacuole fills with water
- nutr: food vacuoles (yeast enters thru oral grove)
- excre: plasma membrane excreting yeast/waste
- resp: responds to cotton fiber + increase in food
- growth: will get larger
compare light microscopes vs electron microscopes
Light
- uses light to bounce thru specimen + lens
- cheaper
Electron:
- uses electrons to bounce off specimen (creates more detail)
- more expensive + larger
how to do calculate magnification
mag = size of drawing/size of specimen (actual)
mm x 1000 to get nM (and round to sig figs)
Label and describe functions of the parts of a prokaryote
- pili: used for cell adhesion (look like tentacles)
- nucleoid region: naked loop of DNA
- plasma membrane: control what goes in and out
- ribosomes: location of protein synthesis
- plasmid: to store genes + DNA
- flagela: used for motion
- cell wall: structure and protection
- cytoplasm: fluid, place for enzyme reactions
compare prokaryote vs eukaryote
Similarities
- cytoplasm for reactions
- smallest unit of life
- enzymes
- ribosomes
- plasma membrane
- DNA
Differences
- pro: nucleous region, euk: true nucleous
- pro: no mitochondria, euk: yes mitochondria
- pro: much smaller, euk: larger
- pro: have pili, euk: no pili
- pro: 70s ribosomes, euk: 80s ribosomes
- pro: naked DNA, euk: histone protein DNA
compare plant and animal cells
Animal
- no chloroplasts
- no cell wall
- small to no vacuole
- lysosomes
Plant:
- chloroplasts
- cell wall
- water vacuole
- no lysosomes
Both:
- plasma membrane
- eukaryotic
- nucleolus
- vacuoles
- mitochondria
Compare the Davson-Danielli vs Singer-Nicolson membrane models and the evidence they used
Davson-Danielli
- protein sandwich with phospholipid bilayer in middle
- examined membranes with electron microscopes showing railroad with dark edges (protein) and lighter centers (phospholipid)
Singer-Nicolson
- fluid mosaic model: several kinds of proteins
- proven by the fluidity of the membrane and ability of proteins to mix and flow with others (tagged red and green)
- not a smooth membrane
How do amphipathic (hydrophobic + philic) properties of membranes maintain their structure?
- phospholipids have two ends, hydrophilic (non-polar) tails and hydrophobic (polar) heads
- the heads want to be near water, like outside or cytoplasm, and tails want to be away from water
- creates amphipathic bilayer so they don’t move
what is the role of cholesterol in animal membranes?
- is hydrophobic, so stays near tails
- decreases membrane fluidity in hot temps
- increases fluidity in cold temps
- reduces permeability for hydrophilic ions
define diffusion and osmosis + their lab application
Osmosis
- movement of water across membrane from low solute concentrate to high
- showed by dialysis tubing lab - water passed thru tube to reach corn syrup concentration
Diffusion:
- simple: movement of particles from high to low concentration
- facilitated: simple diffusion through the help of hydrophilic channel or pore in membrane
explain active transport across membranes by simple diffusion and facilitated diffusion
- Active transport cannot use diffusion, particles are going against the concentration gradient
- ATP is used to carry out, particles move thru globular proteins
what is the role of protein pumps + ATP in active transport?
- protein pumps are like channels in the protein, open to ions the cannot pass thru simple diffusion
- ATP allows the pumps to change openings for ions to pass
Outline the 5 steps of the sodium-potassium pump
- three sodium ions enter pump and attach to sides when protein opens to inside of axon
- ATP transfers a phosphate group, changing shape of protein
- pump opens to exterior of axon and ions release
- 2 potassium enter and bind to inside
- binding causes phosphates to release, pump changes shape and potassium ions released to exterior of axon