Cell Bio Exam 2 Lecture 5 Flashcards
(19 cards)
How does confocal microscopy eliminate noise?
A confocal does this by repeatedly focusing a laser trough a pinhole to a defined depth of the sample only.
Both confocal microscopy and deconvolution microscopy seek to _________
eliminate out of plane of focus signals (noise).
How does deconvolution microscopy eliminate noise?
Deconvolution methods take sectioned images without the use of a laser and remove noise using algorithms
You use _____ to determine the ______ of a yeast growth regulating protein
GFP, localization
Total internal reflected fluorescence or TIRF allows
_______
tracking of single proteins in cells (e.g. motors or receptors)
What is FRAP?
FRAP = Fluorescence Recovery After Photobleaching
method for determining movement of proteins by bleaching a pool of molecules in one area and watching the area fill back in (or not)
(protein mobility)
What is FRET?
FRET = Fluorescence Resonance Energy Transfer
method for determining if two proteins are close to one another in cells by exciting a fluoror bound to one and looking for excitation and emission of signal from the other without external excitation
(protein proximity to one another)
In electron microscopy:
Heavy metals are used to “stain” biomolecules
siRNAs are involved in ____ gene regulation
post-transcriptional
RISC complex functions in
regulating different genes by miRNAs encoded by the genome.
CRISPR – Cas9 is also known as
“genome editing”
dominant gain of function versions of a protein may encode
poison subunits or versions
of the protein which are always “on”
Southern Blot = ?
DNA
Northern Blot = ?
RNA
Immunoblot / Western Blot = ?
Protein
What is transcriptomics?
Microarray or chip based analysis of transcription across thousands of genes in the genome
Power in the transcriptomics approach comes from
“parallel analysis”
Agonists are
activator drugs
Antagonists are
inhibitor drugs
