Cell Bio Exam 2 Lecture 5 Flashcards
How does confocal microscopy eliminate noise?
A confocal does this by repeatedly focusing a laser trough a pinhole to a defined depth of the sample only.
Both confocal microscopy and deconvolution microscopy seek to _________
eliminate out of plane of focus signals (noise).
How does deconvolution microscopy eliminate noise?
Deconvolution methods take sectioned images without the use of a laser and remove noise using algorithms
You use _____ to determine the ______ of a yeast growth regulating protein
GFP, localization
Total internal reflected fluorescence or TIRF allows
_______
tracking of single proteins in cells (e.g. motors or receptors)
What is FRAP?
FRAP = Fluorescence Recovery After Photobleaching
method for determining movement of proteins by bleaching a pool of molecules in one area and watching the area fill back in (or not)
(protein mobility)
What is FRET?
FRET = Fluorescence Resonance Energy Transfer
method for determining if two proteins are close to one another in cells by exciting a fluoror bound to one and looking for excitation and emission of signal from the other without external excitation
(protein proximity to one another)
In electron microscopy:
Heavy metals are used to “stain” biomolecules
siRNAs are involved in ____ gene regulation
post-transcriptional
RISC complex functions in
regulating different genes by miRNAs encoded by the genome.
CRISPR – Cas9 is also known as
“genome editing”
dominant gain of function versions of a protein may encode
poison subunits or versions
of the protein which are always “on”
Southern Blot = ?
DNA
Northern Blot = ?
RNA
Immunoblot / Western Blot = ?
Protein
