CC2 Enzymes - Feb 26 quiz Flashcards
6 Enzyme Classes
Class 1 Oxidoreductases
Class 2 Transferases
Class 3 Hydrolases
Class 4 Lyases
Class 5 Isomerases
Class 6 Ligases
ENZYME NOMENCLATURE consisting of 4 numbers separated by periods
Enzyme class
subclass
Sub-subclass
Serial number for substrate
Enzymes follow the ___ kinetics
Michaelis-Menten
Enzymes undergo function as governed by two theories
Fischer’s Lock and Key theory
Koshland’s Induced Fit theory
theory….enzyme (lock) and substrate (key) perfectly fits
Fischer’s Lock and Key theory
theory ….enzyme undergoes conformational changes to accommodate shape of the substrate
Koshland’s Induced Fit theory
factors affecting enzymes (7)
substrate concentration,
enzyme concentration,
pH,
temperature,
cofactors,
inhibitors
Types of Enzyme Assay (3)
● Endpoint Analysis
● Multipoint Assay
● Kinetic Assay
✅● Endpoint Analysis
= reaction is initiated by the addition of substrate
and allowed to proceed for a period of time
= measurement of the product or substrate is done at the end of the reaction
✅ ● Multipoint Assay
= measures the change in concentration of the substance at several intervals during the course of the assay
✅ ● Kinetic Assay
= continuous measurement of change in concentration as a function of time
ENZYME INHIBITION (4)
✅No inhibition – Absence of inhibitor
✅Competitive – inhibitor binds to ACTIVE SITE
✅Noncompetitive – inhibitor binds to ALLOSTERIC SITE
✅Uncompetitive– inhibitor binds to E-S COMPLEX
interpret… CK-MB present and LD
both MI and non MI cases
interpret… CK-MB present and flipped LD
100% predictive value that there is MI
(4) Pronounced elevation of CK (5 or more times normal)
● Duchenne’s muscular dystrophy
● polymyositis
● dermatomyositis
● AMI
Mild or moderate elevation (2-4 times Normal)
● severe exercise, trauma, surgical procedure, IM injections
● delirium tremens, alcoholic myopathy
● MI, severe iochemic injury
● pulmonary infarction
● pulmonary edema
● hypothyroidism
● acute agitated psychoses
Reversible conversion of lactate to pyruvate using NAD+ as cofactor
LACTATE DEHYDROGENASE
LD class heart, brain, RBC
LD1 and LD2
LD class brain kidney lung
LD3
LD class liver, skeletal muscle, kidney
LD4 and LD5
LD Pronounced Elevation (5 or more times Normal)
● Megaloblastic anemia
● Widespread carcinomatosis, esp. hepatic metastases
● Septic shock and hypoxia
● Hepatitis
● Renal infarction
● Thrombotic thrombocytompenic purpura
LD Moderate Elevation (3-5 times Normal)
● MI
● Pulmonary infarction
● Hemolytic conditions
● Leukemia
● IM
● Delirium tremens
——- (a psychotic condition typical of withdrawal in chronic alcoholics, involving tremors, hallucinations, anxiety, and disorientation)
● Muscular dystrophy
LD Slight Elevation (Up to 3 times Normal)
● Most liver diseases
● Nephrotic syndrome ● Hypothyroidism
● Cholongitis
NOTE : Association of LD1 & LD2 is useful in confirming diagnosis
of MI after CK has returned to normal (LD flipped) Note: hemolysis should be absent
NOTE : Association of LD1 & LD2 is useful in confirming diagnosis of MI after CK has returned to normal (LD flipped) Note: hemolysis should be absent
● first protein marker to diffuse out of ischemic muscle cells
● presumptive for AMI (not specific, only sensitive)
MYOGLOBIN
troponin polypeptides … released into circulation following myocardial injury (highly specific)
I & T released into circulation following myocardial injury (highly specific)
✅ skeletal muscles have different troponins
✅ peaks at 4-8 hours; fresh appearance in serum suggests AMI
● Act on compounds with single PO4 groups
● has orthophosphate ester monohydrolase activity
● optimally active at pH 5 (acid phosphatase) most active at pH 9 (alkaline phosphatase)
PHOSPHATASES