Bilirubin Auto Total FS Flashcards
Intended use:
(side note)
Diagnostic reagent for quantitative in vitro determination of total bilirubin in human serum or heparin plasma on automated
photometric systems.
Diagnostic reagent for quantitative in vitro determination of total
bilirubin in human serum or heparin plasma on automated
photometric systems.
In the liver, bilirubin is
conjugated with __
glucoronic acid
High levels of total bilirubin are
observed in 60 – 70% of neonates due to an increased postpartum
breakdown of erythrocytes and because of ___
delayed function of
enzymes for bilirubin degradation.
Method used
Photometric test using 2,4-dichloroaniline (DCA)
Direct bilirubin in presence of diazotized 2,4-dichloroaniline forms a red colored azocompound in acidic solution. A specific mixture of detergents enables a safe determination of the total bilirubin [3].
Reagents
(Components and Concentrations)
✅R1:
(1)…..Phosphate buffer
(50 mmol/L)
(2)…..NaCl — 150 mmol/L
✅R2:
(1)….. 2,4-Dichloroaniline
(5 mmol/L)
(2)…..HCl – 130 mmol/L
Reagents are stable up to the date of expiry indicated on the kit, if
stored at____ and contamination is avoided.
2 – 8°C
May be corrosive to metals.
H290
Causes skin irritation
H315
Causes serious eye
irritation
H319
Keep only in original packaging
P234
very toxic to aquatic life with long lasting
effects
H410
Wash
hands and face thoroughly after handling
P264
Wear protective gloves/protective
clothing/eye protection.
P280
If eye irritation persists:
Get medical advice/attention
P337+P313
Collect spillage.
P391
If in eyes: Rinse cautiously with
water for several minutes. Remove contact lenses, if present
and easy to do.
P305+P351+P338
If eye irritation
persists: Get medical advice/attention.
P337+P313
Stability:
1 day at 20 – 25°C
7 days at 4 – 8°C
6 months at –20°C
Absorb spillage
to prevent material damage.
P390
Specimen
Human serum or heparin plasma
Wavelength
545/658 nm
Temperature
37°C
Sample/Calibrator (??)
Reagent 1 (??)
Reagent 2 (??)
Sample/Calibrator ➡️ 2 µL
Reagent 1 ➡️ 80 µL
Reagent 2 ➡️ 20 µL
Addition reagent 2
Cycle 19 (286 s)
Calculation
With calibrator
Bilirubin [mg/dL] = ΔA Sample/ΔA Cal (multiply) Conc. Cal [mg/dL]
DiaSys TruCal U is recommended for calibration. TruCal U
calibrator values have been made traceable to the______
reference material. Use DiaSys _____for internal quality control. Each laboratory should establish corrective action in case
of deviations in control recovery.
✅NIST SRM 916
✅TruLab N and P
Side note:
Measuring range up to ✅30 mg/dL.
When values exceed this range, samples should be diluted
✅1 + 1 with NaCl solution ✅(9 g/L) and the result multiplied by ✅2
🩵
(Performance Characteristics)
Measuring range up to ___
30 mg/dL.
Interfering substance Interferences
Interferences
≤ 10% up to: ???
Ascorbic acid ➡️ 30 mg/dL
Hemoglobin ➡️500 mg/dL
Lipemia (triglycerides) ➡️1000 mg/dL
Bilirubin Conversion Factor (mg/dL to µmol/L)
Bilirubin [mg/dL] x 17.1
Reference Range in NEONATES
24 h ➡️ < 8.8 [mg/dL]
➡️ < 150[µmol/L]
2nd day ➡️ 1.3 – 11.3 [mg/dL]
➡️ 22 – 193 [µmol/L]
3rd day ➡️ 0.7 – 12.7 [mg/dL]
➡️ 12 – 217 [µmol/L]
4th – 6th day ➡️ 0.1 – 12.6 [mg/dL]
➡️ 1.7 – 216 [µmol/L]
Reference Range in CHILDREN
> 1 month ➡️ 0.2 – 1.0 [mg/dL]
➡️ 3.4 – 17
Reference Range in ADULTS
➡️ 0.1 – 1.2 [mg/dL]
➡️ 1.7 – 21 [µmol/L]
