CC PART 4 - LABORATORY BIOSAFETY Flashcards
“The application of safety and containment measures (practices, equipment, facilities) to minimize or prevent exposure from infectious agents to the person handling it in the laboratory, the laboratory, the building occupants, the community, and the environment.”
LABORATORY BIOSAFETY
Which of the following statements regarding Biosecurity is TRUE?
a. protection and prevention of unauthorized possession, loss, theft, misuse, diversion, and accidental or intentional release if biological agents.
b. framework that describes the use of specific practices, training and safety equipment.
c. refers to specially designed buildings to protect the worker, community, and environment from an accidental exposure or unintentional release of infectious agents and toxins.
d. intentional release or dissemination of biological
agents.
a. protection and prevention of unauthorized possession, loss, theft, misuse, diversion, and accidental or intentional release if biological agents.
Anything that alters the structural component of the laboratory
EXPOSURE CONTROL PLANS
Putting cabinets, divisions, or anything that alters the structural component of the lab
Engineering Controls
(4) EXPOSURE CONTROL PLANS
- Exposure Evaluation
- Engineering Controls
- Work Practice Controls
- Administrative oversight of the program
Basis for creation or revision of policies
RECORD KEEPING LOGBOOK
Must include the following:
1. Type and Brand of device involved in the incident
2. Location of the incident
3. Description of the incident
RECORD KEEPING LOGBOOK
Blood Borne Pathogen that can be transmitted by contact with environmental surfaces?
Hepatitis B
○ HBV blood stain can in surfaces for two weeks, requires household bleach to remove it
Disinfectant used in cleaning spills of blood or body fluids?
Answer: freshly prepared 10% solution of sodium hypochlorite (household bleach).
○ Let the bleach soak up first for 30 mins to 1hr before removing or blotting with paper
ROUTE OF ENTRY FOR OCCUPATIONALLY ACQUIRED BLOODBORNE PATHOGENS?
Percutaneous exposure or
Contamination of non-intact skin or mucous membrane (eyes, nose, mouth)
○ Rule of 3 during needlestick injury
○ 0.3% chance of acquiring HIV
○ 3% chance of acquiring Hepatitis C
○ 30% chance of acquiring Hepatitis B
An MLT student sticks himself with a dirty needle while performing phlebotomy on a combative patient. What should he do?
Follow the Laboratory’s exposure control plan. Purge the puncture site with slight pressure then immediately thoroughly wash
soap and water then apply 70% alcohol, inform the supervisor and complete an incident report.
An approach to infection control to which all human blood, tissue and most fluids are handled as if known to be infectious for the HIV, Hepatitis B, and other Blood Borne Viruses.
UNIVERSAL PRECAUTION
The concept of treating all blood and body fluids as capable of transmitting infectious diseases is?
a. safe practice standards
b. infection control
c. universal precautions
d. safety protocol
c. universal precautions
Regulation that ensures that hazard information is properly
communicated to employees.
HAZARD COMMUNICATION
Ability to cause harm
Hazard
Likelihood that a hazard will occur
Risk
Reduce or eliminate occupational exposure to hazardous chemicals.
CHEMICAL HYGIENE PLAN
● Establish laboratory work methods and safety policies.
● Provide supervision and guidance to employees.
● Provide safety information, training, PPE, and medical surveillance to employees
EMPLOYER’S RESPONSIBILITY
● Provide and maintain equipment and laboratory facilities that are free of recognized hazards and adequate for the tasks required.
● Maintain clean PPE used by more than one person
● Provision of Exposure Control Plan
EMPLOYER’S RESPONSIBILITY
● Know and comply with the established laboratory safe work practices.
● Have a positive attitude toward supervisors, coworkers, facilities, and safety training.
EMPLOYEE’S RESPONSIBILITY
● Be alert and give prompt notification of unsafe conditions
or practices to the immediate supervisor and ensure that unsafe conditions and practices are corrected.
● Engage in the conduct of safe work practices and use of PPE.
EMPLOYEE’S RESPONSIBILITY
● Statement hazard
● Hazard Class
● Safety Precautions
● NFPA code
● Fire Extinguisher Type
● Safety instructions
● Formula weight
● Lot Number
APPROPRIATE SIGNS AND LABELING OF REAGENT
Practice of safety in the laboratory involves?
a. The heads and supervisors only
b. The laboratory aids in handling the laboratory wastes
c. Everyone working in the laboratory
d. The medical technologists doing the tests
c. Everyone working in the laboratory
● Safety Showers
● Eyewash stations
● Fire extinguishers
● Fire Blankets
● Spill kits First aid supplies
● Mechanical Pipetting devices
SAFETY EQUIPMENT
