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CC LEC MOD 2 Flashcards

1
Q

Measures potential voltage between two electrodes

potential or voltage basis to determine concentration of solution

A

POTENTIOMETRY

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2
Q

Used to predict the electrochemical cell potential

A

Nernst equation

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3
Q

TYpes of electrode under potentiometry

A
  1. refereence electrode
  2. indicator electrode
  3. ISE (Ion Selective Electrode)
  4. pH electrode
  5. pCO2 Electrode
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4
Q

Half cell potential that is known constant and sensitive to the composition of the solution

purpose is to deliver constant voltage to the meter device

A

Reference electrode

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5
Q

Types of reference

A

calomel electrode (saturated calomel electrode)
silver chloride electrode

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6
Q

Mercury in contact with solution that is saturated with mercury (I) chloride (calomel)

A

calomel electrode

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7
Q

silver electrode immersed in a solution of potasium chloride that has been saturated with silver chloride

A

Silver cloride electrode

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8
Q

Selwctive to repsponse to changes in the act of the unknown being messured
Unknown analyte of interest

A

Indicator electrode

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9
Q

Capable of responding to one specific ion and is very sensitive

A

ISE

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10
Q

measures hydrogen ion activity

A

pH electrode

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11
Q

has Cl buffer solution that has a known buffer hydrogen ion concentration

A

Glass electrode

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12
Q

component of pH electrode with Internal and external electrode with opening

A

Reference electrode

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13
Q

Contained in a plastic jacket filled with sodium bicarbonate buffer

has a gas permeable membrane (teflon or silicone across its opening)

also known as gas sensing electrode

A

pCO2 electrode

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14
Q

Various ISEs may be covered by immobilized enzymes that can catalyze a specific chemical reaction

A

Enzyme electrode

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15
Q

Measures the quantity in coulumbs needed to convert an analyte to different oxidation state

Electrochemical titration where the titrant is electrochemically generated and the endpoint detected by AMPEROMETRY

A

coulometry

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16
Q

Faradays constant

A

96, 485 C/mol of electrons

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17
Q

Measures the current flow produced by an oxidation-reduction reaction

A

amperometry

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18
Q

coulometric-amperometric chemical titration process for chloride determination

A

cotlove titration

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19
Q

potential applied to an electrochemical cell and the resulting current is measured

analytes can be measured in parts-per-billion

A

Voltammetry

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20
Q

Established in the movement of transport ions

Transport of ions or electrically charged particles according to the electric field established between anode and cathode

A

electrochemical methods

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21
Q

Migration of charged particles/ molecules through a solution under the influence electrical field

A

electrophoresis

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22
Q

what is the principle of electrophoresis

A

When charged molecules are placed in an electric field, they migrate toward either the positive or negative pole according to their charge

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23
Q

has either positive or negative charge

A

ampjoteric

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24
Q

Movement of buffer ions and solvent realtive to the fixed supply

A

electroendosmosis

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25
migration of small ions
iontophoresis
26
migration of macromolecules
Zone or zonal electrophoresis
27
In migration of molecules, rate of migration id directly proportional to__ and inversely prop to__
net charge of particle , size and viscosity
28
Source of the current in electrophoresis
Power supply
29
Carrier of current, protects the samples, maintains pH, it affcets ampholytes charges
buffer
30
support medium is read in a ___
densitometer
31
support medium that is easy to use and low cost, dry and brittle
cellulose acetate
32
support medium that does not bind to proteins and requires small amount
agarose gel
33
separates proteins based on their surface charge and molecular size
starch gel
34
different pore sizes can be layered detect 20 serum protein fraction
polyacrylamide gel
35
Simplest way to detect and visualize test result under UV light
detecting system
36
graph illustration of electrophoresis
electrophoretogram
37
Has the farthest migration and highest peak
albumin
38
Slowest migration in electrophoresis
igG
39
Nephrotic syndrome
dec albumin dec a-1, beta, gamma inc a-2
40
acute phase reactants
alpha and a-2 globulin increased
41
M-spike
multiple myeloma, albumin and gamma inc the rest dec
42
beta gamma bridge
active cirrhosis
43
uses homogenous solutions without a support medium convective forces prevented resolution into distinct zones
use of anti-convection support medium
44
bulk of liquid toward the cathode upon application of electric field
electro-osmotic flow
45
Separation of soluble components in a solution by specific differences in physio-chemical properties of the different constituents
chromatography
46
through which the mobile phase flows
stationary phase
47
mobile phase leaving the column/ "eluate"
separate molecules
48
compunds within the solution thatv travel the furthest
non polar
49
glass or plastic plates with a thin layer of adsorbent material
TLC
50
Running of a test one test at a time in a batch
Centrifugal flow analysis
51
use of air bubble
continuous flow analysis
52
can run mult test one sample at a time or mult samples one test at a time
discrete analysis
53
lab test performed outside the central core of lab
POCT
54
separates parent molecular ions and their fragmentations
mass analyzer
55
fragmentation and ionization of molecules using a suitable source of energy
mass spectrometry
56
separates enantiomers
chiral chromatography
57
separation of ions and molecules that can be easily ionized
ion-exchange chromatography
58
GIves better separation of thermolabile compunds
Liquid chromatography
59
detects non-volatile substances in bodily fluids
LC-MS
60
heart of chromatograph
column
61
gas transports the sample components through column
carrier gas and regulator
62
maintains good temp of column
oven
63
detects separate analytes as they eluate from column
detector

MIDTERMS (6 decks)

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