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MIDTERMS > CC LEC MOD 2 > Flashcards

CC LEC MOD 2 Flashcards

1
Q

Measures potential voltage between two electrodes

potential or voltage basis to determine concentration of solution

A

POTENTIOMETRY

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2
Q

Used to predict the electrochemical cell potential

A

Nernst equation

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3
Q

TYpes of electrode under potentiometry

A
  1. refereence electrode
  2. indicator electrode
  3. ISE (Ion Selective Electrode)
  4. pH electrode
  5. pCO2 Electrode
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4
Q

Half cell potential that is known constant and sensitive to the composition of the solution

purpose is to deliver constant voltage to the meter device

A

Reference electrode

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5
Q

Types of reference

A

calomel electrode (saturated calomel electrode)
silver chloride electrode

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6
Q

Mercury in contact with solution that is saturated with mercury (I) chloride (calomel)

A

calomel electrode

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7
Q

silver electrode immersed in a solution of potasium chloride that has been saturated with silver chloride

A

Silver cloride electrode

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8
Q

Selwctive to repsponse to changes in the act of the unknown being messured
Unknown analyte of interest

A

Indicator electrode

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9
Q

Capable of responding to one specific ion and is very sensitive

A

ISE

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10
Q

measures hydrogen ion activity

A

pH electrode

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11
Q

has Cl buffer solution that has a known buffer hydrogen ion concentration

A

Glass electrode

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12
Q

component of pH electrode with Internal and external electrode with opening

A

Reference electrode

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13
Q

Contained in a plastic jacket filled with sodium bicarbonate buffer

has a gas permeable membrane (teflon or silicone across its opening)

also known as gas sensing electrode

A

pCO2 electrode

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14
Q

Various ISEs may be covered by immobilized enzymes that can catalyze a specific chemical reaction

A

Enzyme electrode

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15
Q

Measures the quantity in coulumbs needed to convert an analyte to different oxidation state

Electrochemical titration where the titrant is electrochemically generated and the endpoint detected by AMPEROMETRY

A

coulometry

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16
Q

Faradays constant

A

96, 485 C/mol of electrons

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17
Q

Measures the current flow produced by an oxidation-reduction reaction

A

amperometry

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18
Q

coulometric-amperometric chemical titration process for chloride determination

A

cotlove titration

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19
Q

potential applied to an electrochemical cell and the resulting current is measured

analytes can be measured in parts-per-billion

A

Voltammetry

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20
Q

Established in the movement of transport ions

Transport of ions or electrically charged particles according to the electric field established between anode and cathode

A

electrochemical methods

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21
Q

Migration of charged particles/ molecules through a solution under the influence electrical field

A

electrophoresis

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22
Q

what is the principle of electrophoresis

A

When charged molecules are placed in an electric field, they migrate toward either the positive or negative pole according to their charge

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23
Q

has either positive or negative charge

A

ampjoteric

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24
Q

Movement of buffer ions and solvent realtive to the fixed supply

A

electroendosmosis

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25
Q

migration of small ions

A

iontophoresis

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26
Q

migration of macromolecules

A

Zone or zonal electrophoresis

27
Q

In migration of molecules, rate of migration id directly proportional to__ and inversely prop to__

A

net charge of particle , size and viscosity

28
Q

Source of the current in electrophoresis

A

Power supply

29
Q

Carrier of current, protects the samples, maintains pH, it affcets ampholytes charges

A

buffer

30
Q

support medium is read in a ___

A

densitometer

31
Q

support medium that is easy to use and low cost, dry and brittle

A

cellulose acetate

32
Q

support medium that does not bind to proteins and requires small amount

A

agarose gel

33
Q

separates proteins based on their surface charge and molecular size

A

starch gel

34
Q

different pore sizes can be layered
detect 20 serum protein fraction

A

polyacrylamide gel

35
Q

Simplest way to detect and visualize test result under UV light

A

detecting system

36
Q

graph illustration of electrophoresis

A

electrophoretogram

37
Q

Has the farthest migration and highest peak

A

albumin

38
Q

Slowest migration in electrophoresis

A

igG

39
Q

Nephrotic syndrome

A

dec albumin
dec a-1, beta, gamma
inc a-2

40
Q

acute phase reactants

A

alpha and a-2 globulin increased

41
Q

M-spike

A

multiple myeloma, albumin and gamma inc the rest dec

42
Q

beta gamma bridge

A

active cirrhosis

43
Q

uses homogenous solutions without a support medium
convective forces prevented resolution into distinct zones

A

use of anti-convection support medium

44
Q

bulk of liquid toward the cathode upon application of electric field

A

electro-osmotic flow

45
Q

Separation of soluble components in a solution by specific differences in physio-chemical properties of the different constituents

A

chromatography

46
Q

through which the mobile phase flows

A

stationary phase

47
Q

mobile phase leaving the column/ “eluate”

A

separate molecules

48
Q

compunds within the solution thatv travel the furthest

A

non polar

49
Q

glass or plastic plates with a thin layer of adsorbent material

A

TLC

50
Q

Running of a test one test at a time in a batch

A

Centrifugal flow analysis

51
Q

use of air bubble

A

continuous flow analysis

52
Q

can run mult test one sample at a time or mult samples one test at a time

A

discrete analysis

53
Q

lab test performed outside the central core of lab

A

POCT

54
Q

separates parent molecular ions and their fragmentations

A

mass analyzer

55
Q

fragmentation and ionization of molecules using a suitable source of energy

A

mass spectrometry

56
Q

separates enantiomers

A

chiral chromatography

57
Q

separation of ions and molecules that can be easily ionized

A

ion-exchange chromatography

58
Q

GIves better separation of thermolabile compunds

A

Liquid chromatography

59
Q

detects non-volatile substances in bodily fluids

A

LC-MS

60
Q

heart of chromatograph

A

column

61
Q

gas transports the sample components through column

A

carrier gas and regulator

62
Q

maintains good temp of column

A

oven

63
Q

detects separate analytes as they eluate from column

A

detector

MIDTERMS (6 decks)

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