CBS blood donor products Flashcards
2 canadian systems for blood
hema quebec and Canadian blood services replaced the red cross bc of the krever inquiry
how frequently can men & women donate blood
volume and time
56 days for men ( 7 x yr)
84 days for women ( 4 x yr)
- women have less iron so less donations
480mL collected in 10-15 mins
apheresis platelets and plasma can be donated every
14 days for plts
7 days for plasma
donor eligibility
17 yrs old
at least 110lbs
hgb(g/L) 125(F) 130 (M)
temporary defferal from donating
tattoos, peircings- 3 months
travel risk for malaria or zika
aspirin- affects plt function ( 72 hrs)
permenant deferral from donating
taking money or drugs in exchange for sex since 1977 or using IV street drugs
lived in an area with vCJD outbreak
mandatory donor testing
ABO/ Rh and other antigens
antibody screen
serological/ NAT testing for
- HIV 1& 2
- hep B & C
- HTLV
- WNV ( june- nov)
- syphilis ( treponema pallidum )
additional testing
chagas ( treonema cruzi)- if donors mother or grandmother resided in central/south america
out of season WNV - travel risk
CMV- for fetal intrauterine transfusion
testing for plts
plts are stored in warm temps ( more prone to bacterial growth)
therefore they are tested 36 hrs after collection and are in culture bottles for up to 7 days
plasma testing
pooled plasma for products such as IVIG are tested for parvovirus B19
B19 causes fifth disease
anticoagulants in blood and purpose of each
480mL of blood collected with 70 mL of CPD ( citrate, phosphate, dextrose)
sodium citrate: binds calcium ( prevents coagulation )
citric acid: creates acidic environment to slow down glycolysis
monobasic sodium phosphate: buffer to maintain pH
dextrose: provides source of sugar for cell metabolism
2 methods for separating whole blood
- buffy coat method ( B1)
2.whole blood filtration ( B2)
buffy coat method(B1)
separated into:
blood ( filtered)
plasma unit
buffy coat( 4 buffy coat donors and 1 plasma unit for pooled plt unit)
whole blood method ( B2)
filtered first then separated into :
rbc unit
plasma unit ( frozen )
- frozen plasma
- exported for further manufacturing
- first step in cryo
packed RBC unit after processing
- volume
- storage additive
~290mL
- SAGM ( saline-adenine-glucose-mannitol)
purpose of SAGM
prevents storage lesions
- maintains ATP and 2-3 DPG to help with oxygen levels and reduce potassium leakage from inside cells
storage for RBC units and shelf life
stored 1-6 degrees or 1-10 if in transit for less than 24hrs
shelf life 42 days
timeline to transfuse RBCs
once breeched must be transfused in 24hrs if refrigerated or within 4 hrs if above 6 degrees
if removed from temp control and not transfused must be returned within 1 hr
frozen RBC units
frozen with glycerol for up to 10 years below -65 degrees ( done for rare phenotypes)
before transfusion RBCs thawed and washed to deglycerolize; must be transfused in 24hrs
disoloration acceptable for transfusion
plasma discoloration
-hemolysis up to 0.8%
- icterus
-green ( oral contraceptives
-orange ( vit A/ carrots )
- lipemia
how to tell if bacterial contamination is in RBCs
excessive air bubbles
dark purple or black
- NOT accceptable
acceptable particulate matter
white particulate matter - lipid material that may dissipate and change with temp
NOT acceptable particulate matter
particulates that dont dissipate with gentle manipulation
- clots/ fibrin strands
- cellular aggregates
- cold agglutinin masses
storage and shelf life of plts
stored at 20- 24 degrees under continuous agitation in gas permeable containers ( CO2 from metabolic activity casues pH to drop)
shelf life : 7 days
when would plts be irradiated
for immunocompromised patients
apheresis fresh frozen plasma
collected by plasmaphersis and frozen within 8 hrs
frozen plasma
collected from RBC unit frozen within 24hrs
plasma storage
shelf life of 12 months -18 degrees
once defrosted dont refreeze
cryo preparation
cryo is frozen plasma defrosted separated and refozen as cryosupernatant and cryoprecipitate
solvent detergent plasma prep, shelf life, and when to use
plasma pooled from many donors and tested
shelf life 4 years
certain conditions - TTP & HUS
directed donations
collected from a parent or guardian and given to their minor child
this has increased risk to the patient
Autologous donations
preoperative patient donating blood weeks prior to surgery for self transfusion
- ONLY cases where whole blood is transfused
may be necessary for extremely rare blood types however usually causes pre- operative anemia ( counter productive)
stem cells
CBS has a stem cell registry searching for compatible stem cells ( cord blood) & bone marrow donors
