Case Unit 3 - Screens

Question 1

What is an assay?

Answer 1

Simply a test to measure for something

Question 2

What is a screen?

Answer 2

Process of testing many things at once using an assay (need for scalability)

Question 3

What are the advantages of using a screen?

Answer 3

Don’t need to know anything
Only identify things that work
Quick and free from bias

Question 4

Describe the steps to complete an artificial genetic screen

Answer 4

1. Decide which process you want to investigate
2. Choose a model organism e.g. yeast, worm, zebrafish, drosophila
3. Mutate genes
4. See if the process still works
5. If not, identify the mutated genes
6. Can reintroduce wt gene to see if rescue event occurs

Question 5

How can we mutate/break individual genes?

Answer 5

RNAi - knocks down mRNA

CRISPR