Cardiac perfusion

Question 1

histology

Answer 1

the study of microscopic anatomy

Question 2

How is tissue prepared using light microscopy

Answer 2

tissue is fied with 4% formaldehyde in a phosphate buffer.

Question 3

What does fixing a tissue slice do?

Answer 3

cross links proteins by the formation of methylene bridges so that tissue doesn’t degrade

Question 4

How was this tissue we stained with cresyl violet fixed hint: by ___

Answer 4

by cardiac perfusion with 4% formaldehyde

Question 5

how did we visualize our cresyl violet stained tissue

Answer 5

light microscopy

Question 6

What is the first step in cardiac perfusion

Answer 6

deeply anesthetizing the animal

Question 7

What is the first step in opening the thoracic cavity in cardiac perfusion

Answer 7

making a lateral incision through the integument and abdominal wall

Question 8

what does integument mean

Answer 8

outter covering of animal

Question 9

what organ is exposed in cardiac perfusion

Answer 9

the heart

Question 10

how is the heart exposed

Answer 10

parrallel cuts are made on either side of the ribs up to the collar bone and the tip of the sternum is clamped over the head with the hemostat

Question 11

During cardiac perfusion the needle is inserted where

Answer 11

the left ventricle

Question 12

during cardiac perfusion the perfusion needle is inserted through the left ventricle and into what

Answer 12

the ascending aorta

Question 13

During cardiac perfusion a large incision is made where and what is it’s purpose

Answer 13

a large incision is made in he right atrium. it’s purpose is to all ow the blood and perfusate to drain

Question 14

during cardiac perfusion fixation what system of the body is used to fix the animal

Answer 14

vascular system

Question 15

What substances are used to clear the blood during cardiac perfusion

Answer 15

ice cold ~100ml .9% saline and .1-1% sodium nitrite

Question 16

what does the clearing of the blood during cardiac perfusion cause in the liver and extremities

Answer 16

it causes them to be pale after about 5 min

Question 17

what does sodium nitrite do that helps with clearing the blood?

Answer 17

dilates the blood vessels

Question 18

after clearing of the blood during cardiac perfusion what is perfused into the vascular system (general term)

Answer 18

fixative

Question 19

during cardiac perfusion what is the fixative composed of

Answer 19

400-500ml ice cold 4% formaldehyde in a sodium phosphate buffer

Question 20

how can you tell when the fixative begins working during cardiac perfusion

Answer 20

the body begins to have tremors and then it will become rigid (20-30min)

Question 21

what is used to peel back the skull during the rat brain dissection after cardiac perfusion

Answer 21

a Rongeur