Cardiac perfusion Flashcards
histology
the study of microscopic anatomy
How is tissue prepared using light microscopy
tissue is fied with 4% formaldehyde in a phosphate buffer.
What does fixing a tissue slice do?
cross links proteins by the formation of methylene bridges so that tissue doesn’t degrade
How was this tissue we stained with cresyl violet fixed hint: by ___
by cardiac perfusion with 4% formaldehyde
how did we visualize our cresyl violet stained tissue
light microscopy
What is the first step in cardiac perfusion
deeply anesthetizing the animal
What is the first step in opening the thoracic cavity in cardiac perfusion
making a lateral incision through the integument and abdominal wall
what does integument mean
outter covering of animal
what organ is exposed in cardiac perfusion
the heart
how is the heart exposed
parrallel cuts are made on either side of the ribs up to the collar bone and the tip of the sternum is clamped over the head with the hemostat
During cardiac perfusion the needle is inserted where
the left ventricle
during cardiac perfusion the perfusion needle is inserted through the left ventricle and into what
the ascending aorta
During cardiac perfusion a large incision is made where and what is it’s purpose
a large incision is made in he right atrium. it’s purpose is to all ow the blood and perfusate to drain
during cardiac perfusion fixation what system of the body is used to fix the animal
vascular system
What substances are used to clear the blood during cardiac perfusion
ice cold ~100ml .9% saline and .1-1% sodium nitrite
what does the clearing of the blood during cardiac perfusion cause in the liver and extremities
it causes them to be pale after about 5 min
what does sodium nitrite do that helps with clearing the blood?
dilates the blood vessels
after clearing of the blood during cardiac perfusion what is perfused into the vascular system (general term)
fixative
during cardiac perfusion what is the fixative composed of
400-500ml ice cold 4% formaldehyde in a sodium phosphate buffer
how can you tell when the fixative begins working during cardiac perfusion
the body begins to have tremors and then it will become rigid (20-30min)
what is used to peel back the skull during the rat brain dissection after cardiac perfusion
a Rongeur
