Carbohydrates Flashcards
What is the test for reducing sugars?
Benedict’s test
how is the benedict’s test carried out?
Add liquid food sample or ground food sample
Add an equal volume of benedict’s reagent
Heat the mixture until gently in a water bath boiling (5mins)
what colour will should the solution turn when doing a reducing sugar test
orange brown or red
glucose + glucose forms what
maltose
Glucose +fructose forms what
sucrose
Glucose + galactose forms what
lactose
what type of reaction occurs when monosaccharides join
condensation
What bonds from between monosaccharides
glycosidic
what is the process for testing for non-reducing sugars
complete reducing sugars test
if solution stays blue do the following steps
add equal amounts of food sample and dilute hydrochloric acid
add sodium hydrogencarbonate solution to neutralise
test with PH paper for alkaline PH
add 2cm of benedict’s solution
heat in water bath for 5mins should turn orange or red
how is starch tested for
add two drops of iodine to 2cm sample
starch is present if a black or blue colouration appears
Amylase breaks down what into what
starch into maltose
maltase breaks down what into what
maltose into alpha glucose
