Carbohydrates Flashcards
Conversion Factor of glucose
0.0555
Excess glucose is stored in the _ in the form of _
Liver
Glycogen
Monosaccharides
Glucose
Galactose
Fructose
Disaccharides
Maltose
Lactose
Sucrose
Combination of Maltose
2 Glucose
Combination of Lactose
Glucose
Galactose
Combination of Sucrose
Glucose
Sucrose
The only non-reducing sugar
Fructose
Breakdown of glucose into lactate and pyruvate
Glycolysis
Production of glycogen
Glycogenesis
Breakdown of glycogen into glucose
Glycogenolysis
Production of glucose products from non-carbohydrate sources
Gluconeogenesis
Production of fat from excess glucose
Lipogenesis
Breakdown of triglycerides to produce ketone bodies
Lipolysis
How many ATPs can NADH produce?
3 ATP
How many ATPs can FADH2 produce?
2 ATP
How many ATPs are produced in bacterial ATP production?
38 ATP
A hypoglycemic agent that promotes glycogenesis and lipogenesis; released in the beta cells of pancreas
Insulin
A hyperglycemic agent that promotes glycogenolysis and gluconeogenesis; produced by the alpha cells of pancreas
Glucagon
A hyperglycemic agent that stimulates glycogenolysis and lipolysis; inhibits insulin production; produced in the adrenal medulla; known for adrenaline rush
Epinephrine
A hyperglycemic agent that stimulates glycogenolysis, lipolysis and gluconeogenesis; release is stimulated by ACTH; produced by adrenal cortex (pituitary gland)
Cortisol
A hyperglycemic agent that inhibits secretion of insulin, glucagon, and growth hormone; command center; produced by the delta cells of the pancreas
Somatostatin
A hyperglycemic agent that stimulates glycolysis; produced by the anterior pituitary gland; prevents glucose entry
Growth Hormone
A hyperglycemic agent that stimulates glycogenolysis and gluconeogenesis; produced by the thyroid gland; increased intestinal absorption of glucose
Thyroxine
Fasting period for glucose determination
8-10 hours
Tubes used for glucose determination
Non-additive
Fluoride
Iodoacetate (anti-glycolytic)
Glucose load for adult
75g
Glucose load for pregnant
100g
Glucose load should be consumed within _
15 minutes
How many days of high carbohydrate diet for tolerance testing?
3-day
Normal Glucose Tolerance range
</= 70-140mg/dL (</= 7.8 mmol/L)
Impaired Glucose Tolerance Range
</= 140-199mg/dL (7.8-11.1 mmol/L)
Provision Diabetes Diagnosis Range
> /= 200mg/dL (>/=11.1 mmol/L)
Enzyme that converts glucose to gluconic acid
Glucose oxidase
Reduced chromogen is converted to oxidized chromogen by?
Peroxidase
Oxidized chromogen is - in color
Reddish-purple
Glucose is converted into glucose-6-phosphate via?
Hexokinase
G-6-PO4 is converted to NADPH and 6-phosphogluconate via?
G-6-PD
How many months of value can HbA1c can cover?
2-3 months
Normal range of HbA1c
4-6%
Machine or method of analysis based on charge differences between glycosylated and non-glycosylated hemoglobin
Cation-exchange chromatography
Isoelectric Focusing
Electrophoresis
Machine or method of analysis based on structural characteristics of glycogroups on hemoglobin
Affinity chromatography
Immunoassay
An increase in plasma glucose levels
Hyperglycemia
A group of metabolic diseases
characterized by hyperglycemia resulting from defects in insulin secretion, insulin action or both
Diabetes Mellitus
Hallmarks for Diabetes Mellitus
Polyuria
Polydipsia
Polyphagia
Insulin-dependent; Cannot produce insulin at all; child onset
Type 1 DM
Non-insulin dependent; Can produce insulin but due to high sugar level, sugar become resistant to effects of insulin; adult onset
Type 2 DM
Reaction to body becoming slightly acidic due
to ketoacidosis by kidney (blood pH)
Kussmaul-Kien respiration (hyperventilation)
Neurotransmitter for pancreatic function
Glutamic acid decarboxylase autoantibodies
Enzymes that facilitates production of β-cell (insulin)
Tyrosine phosphatase IA-2 and IA-2B
autoantibodies
In Type II DM, glucose and plasma osmolality can elevate up to
1000 mg/dL
320 mOsm/dL
Markers for Nephropathy or Chronic Kidney
Disease (kidney function)
Elevated BUN and Creatinine
Low blood glucose concentration
Typical hypoglycemia symptoms after fasting
and/or intense exercise
Symptoms alleviated by glucose
administration
Whipple’s Triad
Disorders of Carbohydrate Metabolism: Glucose-6-phosphatase
Ia / Von Gierke
Disorders of Carbohydrate Metabolism: Amylo-1,4-glucosidase / Acid maltase
II / Pompei
Disorders of Carbohydrate Metabolism: Amylo-1,6-glucosidase / Debrancher enzyme
III / Cori-Forbes
Disorders of Carbohydrate Metabolism: Amylopectinase / Glycogen branching enzyme
IV / Andersen
Disorders of Carbohydrate Metabolism: Muscle phosphorylase
V / McArdle
Disorders of Carbohydrate Metabolism: Liver glycogen phosphorylase
VI / Hers
Disorders of Carbohydrate Metabolism: Phosphofructokinase
VII / Tauri
Disorders of Carbohydrate Metabolism: Glycogen transporter 2
XI / Fanconi-Bickel
Disorders of Carbohydrate Metabolism: Glycogen synthetase
0
Disorders of Carbohydrate Metabolism: Galactose-1-phosphate uridyltransferase
Galactosemia (NBS)
Cuprous ions + phosphomolybdate that produces blue complex
Folin-Wu
Cuprous ions + arsenomolybdat that produces blue complex
Nelson-Somogyi
Cuprous ions + neocuproine that produces yellow complex
Neocuproine
Folin-Wu reagent + citrate
Benedict’s
Folin-Wu reagent + tartrate
Fehling’s
Yellow ferricyanide; Known as reverse colorimetry
Hagedorn-Jensen
Aromatic amines + glucose → glycosamine + green Schiff’s base
Ortho-toluidine / Dubowski